Production of a novel opine dehydrogenase

Opine dehydrogenases are a family of NAD(P)H dependent oxidoreductases, which catalyze the reductive condensation of an α amino group from an amino acid with an αketo acid during anaerobic glycolysis by regenerating NAD. They are widespread in cephalopods and mollusks. Opines are associated with crown gall tumor pathogenesis caused by A. tumefaciens providing nutrients to the pathogen, and novel opine compounds acting as metallophores have been identified. Besides, opine-type secondary amine dicarboxylic acids are chiral intermediates of angiotensin-converting enzyme inhibitors. A novel enzyme originating from an extremophile bacterium, with assumed opine dehydrogenase function was successfully expressed in Escherichia coli STAR cells and purified by affinity chromatography. Molecular mass determined by SDS-PAGE was approximately 40 kDa. The activity was measured by using pyruvate and alanine as substrates, by which proved that it has opine dehydrogenase activity

Electrophoresis, as a Method of Rational Application of Medicament of Contemporary Management in Health Care

Preclinical studies of the intake of drugs in the body by electrophoresis and systemic route (intravenous), we examined the concentrations of drugs and the efficiency of the drug application in both ways. We found high-significance concentrations of drugs in the desired tissue, with multiple smaller amounts of drugs administered by electrophoresis, and high values of Students T-test and significance for all investigated drugs p <0.001. Investigating economic costeffectiveness, this methodology of drug intake, we observed by evaluating the cost of medicines of significant material savings with drug application with ionophoresis, which is recommended both from the health and material-financial aspect as a rational modern method of drug intake in clinical practice, wherever clinical possibilities allow it