ROLE OF ANTIOXIDANTS AND NUTRITION IN OXIDATIVE STRESS: A REVIEW

Human body is known to experience positive stress that keeps it vigilant and protects it from biological and physical threats. This stress turns negative when a person is confronted with continuous challenges. Also, stress and nutrition have always run in synergism with each other. Nutritional value of a person's diet depends on the overall mixture or balance of food that is eaten over a period of time, as well as on the needs of the individual. The body relies on obtaining its anti-oxidants from food and other supplements. These nutritional requirements of antioxidants are enormously altered in diseased conditions. In view of the immense medicinal importance of antioxidants, an effort has been made in this article to bring together information about the role of nutrition and antioxidants such as carotenoids, vitamins, some important minerals in combating free radical generation and in general, boosting normal immune mechanisms. The present review is an attempt to generate interest in the scientific fraternity of their immense potential in preventing and treating several common diseases. Keywords: Antioxidants, Free radicals, Balanced diet, Nutrition, Oxidative stress, Vitamins

The Design, Synthesis, and Evaluation of Diaminopimelic Acid Derivatives as Potential <i>dap</i>F Inhibitors Preventing Lysine Biosynthesis for Antibacterial Activity

We created thiazole and oxazole analogues of diaminopimelic acid (DAP) by replacing its carboxyl groups and substituting sulphur for the central carbon atom. Toxicity, ADME, molecular docking, and in vitro antimicrobial studies of the synthesized compounds were carried out. These compounds displayed significant antibacterial efficacy, with MICs of 70–80 µg/mL against all tested bacteria. Comparative values of the MIC, MBC, and ZOI of the synthesized compound were noticed when compared with ciprofloxacin. At 200 µg/mL, thio-DAP (1) had a ZOI of 22.67 ± 0.58, while ciprofloxacin had a ZOI of 23.67 ± 0.58. To synthesize thio-DAP (1) and oxa-DAP (2), l-cysteine was used as a precursor for the L-stereocenter (l-cysteine), which is recognized by the dapF enzyme’s active site and selectively binds to the ligand’s L-stereocenter. Docking studies of these compounds were carried out using the programme version 11.5 Schrodinger to reveal the hydrophobic and hydrophilic properties of these complexes. The docking scores of compounds one and two were −9.823 and −10.098 kcal/mol, respectively, as compared with LL-DAP (−9.426 kcal/mol.). This suggests that compounds one and two interact more precisely with dapF than LL-DAP. Chemicals one and two were synthesized via the SBDD (structure-based drug design) approach and these act as inhibitors of the dapF in the lysine pathway of bacterial cell wall synthesis