MOESM8 of A transient transformation system for gene characterization in upland cotton (Gossypium hirsutum)

Additional file 8. Transformation efficiency

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Additional file 10. GUS staining of plants transiently expressing GhGPX1 promoter constructs using Agrobacterium EHA105-mediated transient transformation at different times after inoculation

MOESM2 of A transient transformation system for gene characterization in upland cotton (Gossypium hirsutum)

Additional file 2. Pictorial representation of the Agrobacterium-mediated transient transformation of G. hirsutum

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Additional file 4. Relative GhWRKY40 expression levels in EHA105 Agrobacterium-inoculated G. hirsutum plants under different transformation conditions

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Additional file 6. GUS staining of transiently transformed cotton seedlings

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Additional file 5. Transient transformation of cotton seedlings with GhGPX1pro-GUS under vacuum infiltration with or without Agrobacterium in the transformation solution

MOESM9 of A transient transformation system for gene characterization in upland cotton (Gossypium hirsutum)

Additional file 9. Quantification of relative expression levels for GhGPX1 and GhWRKY40 in Agrobacterium-inoculated G. hirsutum plants using two different endogenous reference genes

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Additional file 3. Transient GUS expression in cotton plants by GhGPX1pro-GUS-contained EHA105

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Additional file 7. GUS staining of G. hirsutum cultivars CCRI36, Coker201 and TM-1 after transient transformation with A. tumefaciens EHA105 containing the GhGPX1pro-GUS vector

MOESM11 of A transient transformation system for gene characterization in upland cotton (Gossypium hirsutum)

Additional file 11. Subcellular localization of GFP-tagged GhGPX1 in G. hirsutum plant leaves using Agrobacterium GV3101-mediated transient transformation