The Penn Science Teacher Institute: A Proven Model

The University of Pennsylvania’s Master of Chemistry Education (MCE) program graduated five cohorts of approximately twenty teachers between 2002 and 2006. One year after the teachers in the last cohort earned their degrees, the Penn Science Teacher Institute (Penn STI) initiated a follow-up study to ascertain if the goals of the MCE program had been sustained. For example, were the teachers incorporating updated content knowledge into their lessons and were their students learning more chemistry? A total of seventy-four of the eighty-two graduates participated in some aspect of this study. Because baseline data were not available for the MCE teachers and their students, baseline data from a comparable group of chemistry teachers enrolled in the first cohort of the Penn STI program and their students were used in some analyses. Among other findings, the data indicate that MCE met its goals: 1) to improve the chemistry content knowledge of its teacher participants; 2) to increase the use of research-based instruction in their classrooms; and, 3) to improve student achievement in chemistry (students of MCE graduates scored significantly higher than the comparison group)

Geologic application of thermal inertia imaging using HCMM data

Three test sites in the western US were selected to discriminate among surface geologic materials on the basis of their thermal properties as determined from HCMM data. Attempts to determine quantitatively accurate thermal inertia values from HCMM digital data met with only partial success due to the effects of sensor miscalibrations, radiative transfer in the atmosphere, and varying meteorology and elevation across a scene. In most instances, apparent thermal inertia was found to be an excellent qualitative representation of true thermal inertia. Computer processing of digital day and night HCMM data allowed construction of geologically useful images. At some test sites, more information was provided by data than LANDSAT data. Soil moisture effects and differences in spectrally dark materials were more effectively displayed using the thermal data

Random geometric complexes

We study the expected topological properties of Cech and Vietoris-Rips complexes built on i.i.d. random points in R^d. We find higher dimensional analogues of known results for connectivity and component counts for random geometric graphs. However, higher homology H_k is not monotone when k > 0. In particular for every k > 0 we exhibit two thresholds, one where homology passes from vanishing to nonvanishing, and another where it passes back to vanishing. We give asymptotic formulas for the expectation of the Betti numbers in the sparser regimes, and bounds in the denser regimes. The main technical contribution of the article is in the application of discrete Morse theory in geometric probability.Comment: 26 pages, 3 figures, final revisions, to appear in Discrete & Computational Geometr

Auto- und Heterotrophic Respiration in the Hohenheim Climate Change Experiment - The Importance of Temperature Change and Vegetation Period

Current Climate change (CC) research in soil science mainly focusses on natural ecosystems, without considering the potential of agro-ecosystems for feedback mechanisms to CC and CC mitigation through Carbon(C)-sequestration. We expect that CC induces increasing water limitation under elevated temperature, lowers the intensity of soil respiration and changes the ratio between the amount of root-dependent and basal soil respiration. Such changes might be due to differences in the intrinsic temperature and moisture sensitivity of microbial and root respiration and due to altered root exudation. In this project, we focus on CC-induced effects on plant-dependent and basal soil respiration to improve the estimation of long-term soil organic matter stabilization. Within the Hohenheim Climate Change (HoCC) experiment (established in 2008), barley plants were pulse-labelled with 20-atom% 13CO2 for 4 h using ventilated transparent chambers on warmed and control plots in an agricultural field. The labeling was done during three different stages (advanced tillering, booting and grain-filling) of the vegetation period, at which C-sink strength of shoot and root differs according to plant development. CO2-fluxes and isotopic composition were measured in real time in the field for the first 50h (post labeling) using a 13CO2 isotope analyzer. Results from tracing 13C-fluxes will clarify how soil moisture and long-term elevated temperature affect the overall C-balance in agricultural soils in dependence of the vegetation period. This will allow estimations of direction and strength of feedback mechanisms of terrestrial C-cycling under CC. Overall, insights obtained in this project will provide better understanding of the CC impact on and of temperate agricultural production systems

Evaluation of thermal data for geologic applications

Sensitivity studies using thermal models indicated sources of errors in the determination of thermal inertia from HCMM data. Apparent thermal inertia, with only simple atmospheric radiance corrections to the measured surface temperature, would be sufficient for most operational requirements for surface thermal inertia. Thermal data does have additional information about the nature of surface material that is not available in visible and near infrared reflectance data. Color composites of daytime temperature, nighttime temperature, and albedo were often more useful than thermal inertia images alone for discrimination of lithologic boundaries. A modeling study, using the annual heating cycle, indicated the feasibility of looking for geologic features buried under as much as a meter of alluvial material. The spatial resolution of HCMM data is a major limiting factor in the usefulness of the data for geologic applications. Future thermal infrared satellite sensors should provide spatial resolution comparable to that of the LANDSAT data

TDP-43 knockdown impairs neurite outgrowth dependent on its target histone deacetylase 6

<p>Abstract</p> <p>Background</p> <p>Trans-activation response element (TAR) DNA binding protein of 43kDa (TDP-43) is causally related to the neurodegenerative diseases frontotemporal dementia and amyotrophic lateral sclerosis being the hallmark protein in the disease-characteristic neuropathological lesions and via genetic linkage. Histone deacetylase 6 (HDAC6) is an established target of the RNA-binding protein TDP-43. HDAC6 is an unusual cytosolic deacetylase enzyme, central for a variety of pivotal cellular functions including aggregating protein turnover, microtubular dynamics and filopodia formation. All these functions are important in the context of neurodegenerative proteinopathies involving TDP-43. We have previously shown in a human embryonic kidney cell line that TDP-43 knockdown significantly impairs the removal of a toxic, aggregating polyQ ataxin-3 fusion protein in an HDAC6-dependent manner. Here we investigated the influence of TDP-43 and its target HDAC6 on neurite outgrowth.</p> <p>Results</p> <p>Human neuroblastoma SH-SY5Y cells with stably silenced TDP-43 showed a significant reduction of neurite outgrowth induced by retinoic acid and brain-derived neurotrophic factor. Re-transfection with TDP-43 as well as HDAC6 rescued retinoic acid-induced neurite outgrowth. In addition, we show that silencing of HDAC6 alone is sufficient to reduce neurite outgrowth of <it>in vitro </it>differentiated SH-SY5Y cells.</p> <p>Conclusions</p> <p>TDP-43 deficiency leads to impairment of neurite growth in an HDAC6-dependent manner, thereby contributing to neurodegenerative events in TDP-43 diseases.</p

FAS-dependent cell death in α-synuclein transgenic oligodendrocyte models of multiple system atrophy

Multiple system atrophy is a parkinsonian neurodegenerative disorder. It is cytopathologically characterized by accumulation of the protein p25α in cell bodies of oligodendrocytes followed by accumulation of aggregated α-synuclein in so-called glial cytoplasmic inclusions. p25α is a stimulator of α-synuclein aggregation, and coexpression of α-synuclein and p25α in the oligodendroglial OLN-t40-AS cell line causes α-synuclein aggregate-dependent toxicity. In this study, we investigated whether the FAS system is involved in α-synuclein aggregate dependent degeneration in oligodendrocytes and may play a role in multiple system atrophy. Using rat oligodendroglial OLN-t40-AS cells we demonstrate that the cytotoxicity caused by coexpressing α-synuclein and p25α relies on stimulation of the death domain receptor FAS and caspase-8 activation. Using primary oligodendrocytes derived from PLP-α-synuclein transgenic mice we demonstrate that they exist in a sensitized state expressing pro-apoptotic FAS receptor, which makes them sensitive to FAS ligand-mediated apoptosis. Immunoblot analysis shows an increase in FAS in brain extracts from multiple system atrophy cases. Immunohistochemical analysis demonstrated enhanced FAS expression in multiple system atrophy brains notably in oligodendrocytes harboring the earliest stages of glial cytoplasmic inclusion formation. Oligodendroglial FAS expression is an early hallmark of oligodendroglial pathology in multiple system atrophy that mechanistically may be coupled to α-synuclein dependent degeneration and thus represent a potential target for protective intervention

Mirror formation control in the vicinity of an asteroid

Two strategies are presented for the positioning and control of a spacecraft formation designed to focus sunlight onto a point on the surface of asteroid, thereby sublimating the material and ejecting debris creating thrust. In the first approach, the formation is located at artficial equilibrium points around the asteroid and controlled using the force from the solar radiation pressure. The second approach determines the optimal periodic formation orbits, subject to the gravitational perturbations from the asteroid, the solar radiation pressure and the control acceleration derived from a control law

TDP-43 regulates global translational yield by splicing of exon junction complex component SKAR

TDP-43 is linked to neurodegenerative diseases including frontotemporal dementia and amyotrophic lateral sclerosis. Mostly localized in the nucleus, TDP-43 acts in conjunction with other ribonucleoproteins as a splicing co-factor. Several RNA targets of TDP-43 have been identified so far, but its role(s) in pathogenesis remains unclear. Using Affymetrix exon arrays, we have screened for the first time for splicing events upon TDP-43 knockdown. We found alternative splicing of the ribosomal S6 kinase 1 (S6K1) Aly/REF-like target (SKAR) upon TDP-43 knockdown in non-neuronal and neuronal cell lines. Alternative SKAR splicing depended on the first RNA recognition motif (RRM1) of TDP-43 and on 5′-GA-3’ and 5′-UG-3′ repeats within the SKAR pre-mRNA. SKAR is a component of the exon junction complex, which recruits S6K1, thereby facilitating the pioneer round of translation and promoting cell growth. Indeed, we found that expression of the alternatively spliced SKAR enhanced S6K1-dependent signaling pathways and the translational yield of a splice-dependent reporter. Consistent with this, TDP-43 knockdown also increased translational yield and significantly increased cell size. This indicates a novel mechanism of deregulated translational control upon TDP-43 deficiency, which might contribute to pathogenesis of the protein aggregation diseases frontotemporal dementia and amyotrophic lateral sclerosis

T-duality and Differential K-Theory

We give a precise formulation of T-duality for Ramond-Ramond fields. This gives a canonical isomorphism between the "geometrically invariant" subgroups of the twisted differential K-theory of certain principal torus bundles. Our result combines topological T-duality with the Buscher rules found in physics.Comment: 23 pages, typos corrected, submitted to Comm.Math.Phy