Large-scale stem cell production system by newly designed bioreactor

Large scale cell production system is required in drug discovery and cell based therapy. However there are technically challenges in maintaining high quality and low cost for practical biomanufacturing industry. 3D suspension culture is one of attractive process, especially static culture is ultimate free from shear force. Utilizing static 3D suspension culture, automated closed system is newly designed and demonstrated expansion culture over several subcultures. Cell growth curves of this system and conventional manual method have good accordance. The system is also compatible with good scalability for scale up and scale out use. Please click Additional Files below to see the full abstract

Prevention of the initial testosterone surge induced by a luteinizing hormone-releasing hormone analogue in prostate cancer patients:the endocrinological effects of pretreatment with chlormadinone acetate

We investigated the endocrinological effects of pretreatment with chlormadinone acetate (CMA) in preventing the initial testosterone surge induced by a luteinizing hormone-releasing hormone (LH-RH) analogue. A total of 25 patients with previously untreated prostate cancer were included in this study. The patients were randomly assigned to 2 treatment groups:Group1;CMA therapy was begun 4 weeks before the initial LH-RH analogue injection. Group 2;CMA therapy was begun 2 weeks before the initial LH-RH analogue injection. After the initial LH-RH analogue injection, CMA was administered during this study. After LH-RH analogue application, the mean values of serum luteinizing hormone (LH) and testosterone increased in both groups on day 3. However, LH and testosterone levels remained beneath pretreatment values in both groups. The mean relative PSA levels did not significantly increased on day 3 and day 7in both groups. Our results indicate that pretreatment with CMA for 2 weeks was sufficient to prevent the initial testosterone surge in the maximal androgen blockade which was associated with CMA

トクシマシ ゼンリツセンガン ケンシン ノ ゲンキョウ ト カダイ : ダイ2ホウ カコ 3ネンカン ノ ヒカク ケントウ

The aim of this study is to clarify the usefulness of PSA screening to detect prostate cancer subclinically in Tokushima City. Mass screening of serum PSA measurement only was started from ２００２ in Tokushima City. Three years’ results are reported and analyzed in this paper. In ２００２ to ２００４，（９０９９，９３４５，１０６８０） men over ５５ years old were measured serum PSA level with annual check of health condition at various outpatient clinics in Tokushima City. The men with high PSA levels were recommended to visit the urologist for further examinations. By the urologists the men with high PSA levels were diagnosed by careful urologic procedures including DRE, TRUS, accurate prostatic biopsy and more. In （８０１，６０６，７２７） men, PSA levels were over normal range, and （４５５，２５７，３５８） men visited urologic clinics for further examinations. Careful examinations were performed and prostatic biopsies were done in （２３３，１２１，１４１） men. Finally, the prostate cancers were found out in （１２１，６０，５９） men and these men were entered suitable medical treatments immediately. Accordingly， （１．３４％，０．６４％，０．５５％） of （９０９９，９３４５，１０６８０） men with PSA measurements were diagnosed as prostate cancers. In the group of prostate cancer, patient number of early cancer group （Stage B） were （８０：６６．１％，４２：７０．０％，５０：８４．７％），which means that prostate cancers found out by PSA screening might be early curable cancers in many cases. These ３ years’ results indicate that PSA screening is a very useful modality to find out early prostate cancers and contribute the decrease of prostate cancer death in Tokushima City in the future

Development and Performance of Kyoto's X-ray Astronomical SOI pixel (SOIPIX) sensor

We have been developing monolithic active pixel sensors, known as Kyoto's X-ray SOIPIXs, based on the CMOS SOI (silicon-on-insulator) technology for next-generation X-ray astronomy satellites. The event trigger output function implemented in each pixel offers microsecond time resolution and enables reduction of the non-X-ray background that dominates the high X-ray energy band above 5--10 keV. A fully depleted SOI with a thick depletion layer and back illumination offers wide band coverage of 0.3--40 keV. Here, we report recent progress in the X-ray SOIPIX development. In this study, we achieved an energy resolution of 300~eV (FWHM) at 6~keV and a read-out noise of 33~e- (rms) in the frame readout mode, which allows us to clearly resolve Mn-K$\alpha$ and K$\beta$. Moreover, we produced a fully depleted layer with a thickness of $500~{\rm \mu m}$. The event-driven readout mode has already been successfully demonstrated.Comment: 7pages, 12figures, SPIE Astronomical Telescopes and Instrumentation 2014, Montreal, Quebec, Canada. appears as Proc. SPIE 9147, Space Telescopes and Instrumentation 2014: Ultraviolet to Gamma Ra