Going To Pieces

Person playing the piano, with the whole illustration being in many separate pieceshttps://scholarsjunction.msstate.edu/cht-sheet-music/13741/thumbnail.jp

Going to pieces rag : Popular edition ; one step

Gift of Dr. Mary Jane Esplen.Piano. [instrumentation]A flat major [key]Allegretto [tempo]Ragtime. [form/genre]Cartoon piano player, piano and person falling apart. [illustration]Starmer. [engraver]Jerome H. Remick & Co., New York. [dealer stamp]Publisher's advertisement on back cover. [note

Going to pieces : rag one step

For pianoIllustated by StarmerPublisher\u27s advertising includes musical incipi

A transcriptional insulator at the imprinted H19/Igf2 locus

Igf2 and H19 exhibit parent-of-origin-specific monoallelic expression. H19 is expressed from the maternal chromosome and Igf2 from the paternal. The two genes share enhancer elements and monoallelic expression of both genes is dependent on cis-acting sequences upstream of the H19 promoter. In this work we examine the mechanisms by which this region silences the maternal Igf2 allele and we demonstrate that deletion of this region can result in high levels of activation of both H19 and Igf2 from a single chromosome. Moreover, by inserting this cis element between a promoter and its enhancer at a heterologous position, we demonstrate that the sequences carry both insulator activity and the ability to be stably imprinted. We also characterize the insulator in vitro and show that it is neither enhancer nor promoter specific