Assessment and Physicochemical Characterization of Groundwater Quality for Irrigation and Drinking Purposes in Bazer Sakhra (Eastern Area of Algeria)

Twenty groundwater samples were collected and then examined for physical (pH, EC, TDS) and chemical (Na+, K+, Ca2+, Mg2+, HCO3−, Cl−, SO42−, NO3) parameters, followed by multivariate statistics to determine the current state of groundwater quality and to assess the suitability of these resources for drinking and irrigation purposes in the Baser Sakhra area localized in Eastern of Algeria. The analysis carried out showed that the cations trend in most of the groundwater samples is on the order of Ca2+˃Na+˃Mg2+˃K+ and the anions trend is on the order of HCO3-˃SO42-˃Cl-. Stabler diagram demonstrated the predominance of Ca2+- HCO3- hydro-chemical facies (80%). Moreover, the parameters, such as sodium adsorption ratio, percentage sodium, residual sodium carbonate, permeability index, Kelly’s ratio, potential salinity, and magnesium hazard were evaluated for the suitability of groundwater for irrigation. The values obtained in this investigation concluded that most of the groundwater samples are suitable for irrigation. From the other approach, the Water Quality Index (WQI) for drinking was also used in the current study. WQI ranged from 72.46 to 506.426, indicating that 40% of the samples were suitable for drinking however, 60% of them belong to the poor to unsuitable category of drinking water in terms of physicochemical properties, according to the World Health Organization norms

Rosiglitazone Reverses Inflammation in Epididymal White Adipose Tissue in Hormone-Sensitive Lipase-Knockout Mice

Hormone-sensitive lipase (HSL) plays a crucial role in intracellular lipolysis, and loss of HSL leads to diacylglycerol (DAG) accumulation, reduced FA mobilization, and impaired PPARγ signaling. Hsl knockout mice exhibit adipose tissue inflammation, but the underlying mechanisms are still not clear. Here, we investigated if and to what extent HSL loss contributes to endoplasmic reticulum (ER) stress and adipose tissue inflammation in Hsl knockout mice. Furthermore, we were interested in how impaired PPARγ signaling affects the development of inflammation in epididymal white adipose tissue (eWAT) and inguinal white adipose tissue (iWAT) of Hsl knockout mice and if DAG and ceramide accumulation contribute to adipose tissue inflammation and ER stress. Ultrastructural analysis showed a markedly dilated ER in both eWAT and iWAT upon loss of HSL. In addition, Hsl knockout mice exhibited macrophage infiltration and increased F4/80 mRNA expression, a marker of macrophage activation, in eWAT, but not in iWAT. We show that treatment with rosiglitazone, a PPARγ agonist, attenuated macrophage infiltration and ameliorated inflammation of eWAT, but expression of ER stress markers remained unchanged, as did DAG and ceramide levels in eWAT. Taken together, we show that HSL loss promoted ER stress in both eWAT and iWAT of Hsl knockout mice, but inflammation and macrophage infiltration occurred mainly in eWAT. Also, PPARγ activation reversed inflammation but not ER stress and DAG accumulation. These data indicate that neither reduction of DAG levels nor ER stress contribute to the reversal of eWAT inflammation in Hsl knockout mice