Immuno-based detection of extracellular vesicles in urine as diagnostic marker for prostate cancer

Extracellular vesicles (including the subclass exosomes) secreted by cells contain specific proteins and RNA that could be of interest in determining new markers. Isolation/characterization of PCa-derived exosomes from bodily fluids enables us to discover new markers for this disease. Unfortunately, isolation with current techniques (ultracentrifugation) is labor intensive and other techniques are still under development. The goal of our study was to develop a highly sensitive time-resolved fluorescence immunoassay (TR-FIA) for capture/detection of PCa-derived exosomes. In our assay, biotinylated capture antibodies against human CD9 or CD63 were incubated on streptavidin-coated wells. After application of exosomes, Europium-labeled detection antibodies (CD9 or CD63) were added. Cell medium from 37 cell lines was taken to validate this TR-FIA. Urine was collected (after digital rectal exam) from patients with PCa (n=67), men without PCa (n=76). As a control, urine was collected from men after radical prostatectomy (n=13), women (n=16) and patients with prostate cancer without digital rectal exam (n=16). Signal intensities were corrected for urinary PSA and creatinine. This TR-FIA can measure purified exosomes with high sensitivity and minimal background signals. Exosomes can be measured in medium from 37 cell lines and in urine. DRE resulted in a pronounced increase in CD63 signals. After DRE and correction for urinary PSA, CD9 and CD63 were significantly higher in men with PCa. This TR-FIA enabled us to measure exosomes with high sensitivity directly from urine and cell medium. This TR-FIA forms the basis for testing different antibodies directed against exosome membrane markers to generate disease-specific detection assays. What's new? The vesicles cast off by cancer cells could serve as billboards advertising the cancer's presence - if we knew how to read them. Detecting markers in prostate cancer vesicles is currently labor intensive. These authors set out to change that, by developing an immunoaffinity technique to expose these cellular markers more easily. Using the assay, they detected two cell surface proteins, CD9 and CD63, in the urine of men who had prostate cancer. They found far less of the markers in men without cancer, men without prostates, and women, suggesting CD9 and CD63 could be useful prostate cancer markers

Glycoprotein hormone isomorphism and assay discrepancy: The paradigm of luteinizing hormone (LH)

Journal ArticleResearch Support, Non-U.S. Gov'tReviewinfo:eu-repo/semantics/publishe