6 research outputs found
Highly Precise Protein Semisynthesis through Ligation–Desulfurization Chemistry in Combination with Phenacyl Protection of Native Cysteines
No full textEngineered diubiquitin synthesis reveals Lys29-isopeptide specificity of an OTU deubiquitinase
No full textChemical synthesis of histone H2A with methylation at Gln104
No full textHistone H2A methylation at Gln104 (H2AQ104Me) is a new type of histone post-translational modification (PTM) discovered recently. This modification has been found to have significant influence on gene transcription. However, the structural and functional consequence of glutamine methylation on nucleosome remains to be further elucidated. Obtaining of histones with site-specific methylation at glutamine residues might facilitate the studies towards a better understanding of this new PTM. In the present work, total chemical synthesis of H2AQ104Me was carried out through use of the hydrazide-based native chemical ligation. Synthetic histone H2AQ104Me could be successfully incorporated into nucleosomes in vitro and showed a negative influence on the nucleosome stability.National Basic Research Program of China [2013CB932800]; National Natural Science Foundation of China [21532004, 21225207, 81621002]SCI(E)ARTICLE5621-6276
