OCCURRENCE OF ENTEROCOCCI IN RAW PORK AND BEEF AND THEIR ANTIBIOTICS MULTIRESISTANCE

Abstract KROČKO, M., ČANIGOVÁ, M., DUCKOVÁ, V.: Occurrence of enterococci in raw pork and beef and their antibiotics multiresistance. Acta univ. agric. et silvic. Mendel. Brun., 2008, LVI, No. 2, pp. 101-106 The aim of this study was to determine the microbial contamination of raw pork and beef, to estimate the prevalence of enterococci and investigate the antibiotic multiple resistance of enterococci. Total bacterial counts (TBC) were cultured on Plate count agar and enterococci count were cultured on Slanetz -Bartley agar. The TBC a er 24 h post mortem reached the value 3.61 ± 0.78 log cfu . cm −2 for pork and 2.58 ± 0.63 log cfu . cm −2 for beef. The count of enterococci a er 24 h post mortem reached the value 1.98 ± 1.29 log cfu . cm −2 for pork and 1.16 ± 0.47 log cfu . cm −2 for beef. The average value of TBC in pork and beef were signifi cantly (P < 0.05) higher a er 7 days of ripening at 4 °C storage than 24 h post mortem and in pork and beef reached the value 4.69 ± 1.46 log cfu . cm −2 and 4.32 ± 1.44 log cfu . cm −2 resp. The ave rage values of enterococci count a er 7 days of ripening in pork and beef were 2.00 ± 1.27 log cfu . cm −2 and 0.84 ± 0.80 log cfu . cm −2 resp. Susceptibilities of isolated enterococci from pork to antimicrobial agents were tested using the disc diff usion method. Enterococcus faecium was the predominat species out of 50 isolates recovered from pork (72%), followed by E. faecalis (10%). Other enterococcal isolates were identifi ed sporadically (E. mundtti-8%, E. spp.-10%). Out of 50 isolates of enterococci 14% were resistant to vancomycin and 10% were resistant to erythromycin, 18% to ampicillin, 24% to gentamicin and 34% to tetracycline. The calculated antibiotic code profi les indicated that large proportion of enterococci were resistant to all tested antibiotics except vancomycin. Our study suggests that raw pork and beef play a potential role as reservoirs of enterococci multiresistant to antibiotics

Badanie odporności warstwy aluminium na parabolicznej części reflektorów pojazdu

This paper documents a basic quality measurement in an automotive light industry. The experiment is based on a verification of reflective aluminium layer resistance against 100% humidity and higher temperature. This simulates the lifetime of a headlight on vehicle. The goal of this test is to prove that the reflective aluminium layer is able to resist to this environment without any changes in its structure. If any change occurs, the modification of production process will be needed.W pracy udokumentowano podstawowe pomiary jakości w przemyśle motoryzacyjnym. Na podstawie doświadczeń, zweryfikowano odporność odbijającej warstwy aluminium, w 100% wilgotności i w podwyższonej temperaturze. Symulowano żywotność reflektorów w pojeździe. Celem tego testu było wykazanie, że warstwa odbijająca aluminium jest w stanie oprzeć się tym warunkom bez zmiany struktury. W przypadku wystąpienia jakichkolwiek zmian, będzie potrzebna zmiana procesu produkcyjnego