Aging Management in the Design of Nuclear Power Plants

The basic design includes: physical calculations; thermohydraulic calculations; designbasis justification of equipment strength. System-oriented and construction-oriented approaches are used during design and engineering to ensure the reliability of systems. System-oriented approaches include: analysis of prototypes; creation of systems with the minimum necessary number of components, use of redundancy,development of systems that do not allow the dangerous impacts of failure on their components (fail-safe design), optimization of the sequence of systems’ element operation, preliminary calculations of the system under development reliability. This takes into account: R&D results; reference data from other nuclear facilities; aging materials and mechanisms. Aging management program is created during design and operation of a nuclear facility

Monogenoidean parasites of fishes associated with coral reefs in the Ras Mohammed National Park, Egypt: preliminary results

AbstractA parasitological survey of the monogenoids of 14 species of common fishes associated with the local coral reefs of the Ras Mohammed National Park, National Parks of Egypt South Sinai Sector, Egypt, was carried out from May 2003 to May 2005. The monogenoids collected during the survey included 17 species: 8 previously described species, 7 new species in established genera, and 2 new species belonging to new genera

Vibrio cholerae secretion system of the type VI

The review summarizes literature data on the Vibrio cholerae secretion system of the 6th type. This system is a contact-dependent macromolecular mechanism through which bacteria translocate toxic effector proteins into target cells. It is found in many Gram-negative bacteria, including Vibrio cholerae. V. cholerae infects phagocytic amoebae, nematodes, ciliates, bacteria belonging to different species, as well as unrelated strains of V. cholerae using this system. DNA released after lysis of competing bacteria can be taken up by Vibrio cholerae cells, which leads to the acquisition of new genetic material. The type VI secretion system is involved in the infectious process. The destruction of macrophages and microbiota contributes to the active reproduction of the pathogen and colonization of host epitheliocytes, and the production of effector proteins causes the development of diarrhea and intestinal inflammation. Cholera vibrio secretion system of the 6th type has a structure similar to other gram-negative bacteria. The genes encoding the proteins of this system are located in one large region of the second chromosome and in several additional clusters. It has been shown that toxigenic strains of V. cholerae contain an identical set of secretion system genes, while their composition is variable in non-toxigenic isolates. The regulation of secretion system protein expression differs in V. cholerae strains of different toxigenicity, depends on a number of environmental signals, and is associated with other cell regulatory networks. The paper provides experimental data on the analysis of the structure of the global regulatory gene, vasH, of the type VI secretion system in toxigenic and non-toxigenic V. cholerae O1, biovar El Tor strains isolated in the Russian Federation. Thus, the type VI secretion system is an important mechanism that facilitates the survival of V. cholerae in complex communities in vitro, protects against damaging factors of the macroorganism and increases virulence in vivo, and also provides evolutionary transformations of cholera vibrio. Further study of this system will allow a better understanding of the pathogen-host interaction processes, as well as the adaptation mechanisms of V. cholerae in the external environment

Расчетные исследования аэродинамических характеристик винтов мультикоптеров

The article presents the results of computational studies of aerodynamic characteristics for unmanned lift-generating multi-rotor drones of various configurations. The distinctive features of rotors flow were characterized. The rotor interaction was evaluated. The computations were based on the nonlinear rotor blade vortex theory in a non-stationary arrangement. The combinations of four, eight (four coaxial) and fourteen two-bladed rotors at velocity V = 100, 150, 200 km/h were considered. Semi-empirical methods were employed to select the rotor angles of attack, rotation speed, blade installation angles and geometric parameters at the given take-off weight for each combination of rotors and flight airspeed. The computations showed that for a four-rotor lift-generating design (quad-rotor), two rotors installed downstream, depending on the velocity due to the mutual effect, have values of the thrust coefficients ≈10...20% less than those of the rotors located upstream. For a coaxial quad-copter, the effect of the upper front rotor on the upper rear rotor is similar to the effect of the front rotors on the rear ones in a four-rotor lift-generating design. The effect of the upper front rotor on the lower rear rotor does not vary in terms of the average thrust value, and variations are only local in nature. The interaction of other rotors is identical to that of the four-rotor version. A fourteen-rotor lift-generating multi-rotor drone has a complex flow pattern, which generates deviance in the thrust coefficients variation with respect to time. Depending on the mode and rotors location, the average rotor thrust coefficient can vary approximately twice. The computations showed that with the similar geometric parameters and kinematics characteristics, rotors thrust is substantially subject to variation, which causes destabilizing moments to a significant degree without additional control input. Thrust pulsations and, respectively, vibrations grow in intensity as the flight airspeed increases. Probably, the right choice of the rotor configuration and the automatic control system can counterbalance thrust surge by so-called "phasing", i.e. selecting an initial azimuth angle for each rotor.В статье представлены результаты расчетных исследований аэродинамических характеристик несущих систем беспилотных мультикоптеров различных компоновок, выявлены особенности обтекания винтов, проведена оценка взаимовлияния винтов друг на друга. Расчеты основывались на нелинейной лопастной вихревой теории винта в нестационарной постановке. Рассмотрены комбинации из четырех, восьми (четыре соосных) и четырнадцати двухлопастных винтов при скоростях полета V = 100, 150, 200 км/ч. Для каждой комбинации винтов и скорости полета полуэмпирическими методами выбираются: углы атаки винтов, частота вращения, углы установки лопастей и геометрические параметры под заданную взлетную массу аппарата. Расчеты показали, что для несущей системы из четырех винтов (квадрокоптер) два винта, расположенные ниже по потоку, в зависимости от скорости из-за взаимовлияния имеют значения коэффициентов тяги меньше ≈10–20 %, чем винты, находящиеся выше по потоку. Для соосной четырехвинтовой системы влияние верхнего переднего винта на верхний задний винт аналогично влиянию передних винтов на задние в четырехвинтовой несущей системе. Влияние верхнего переднего винта на нижний задний винт по средней величине тяги не меняется, а изменения имеют только локальный характер. Взаимовлияние других винтов друг на друга имеет аналогичный с четырехвинтовым вариантом характер. У мультикоптера с четырнадцативинтовой несущей системой формируется сложная картина течения, которая порождает нерегулярность в изменении коэффициентов тяги по времени. В зависимости от режима и расположения винта значение среднего коэффициента тяги винта может изменяться приблизительно в два раза. Расчеты показали, что при одинаковых геометрических и кинематических параметрах тяга винтов может существенно отличаться, что без дополнительных управляющих воздействий вызывает значительные дестабилизирующие моменты. Пульсации тяги и, соответственно, вибрации возрастают с увеличением скорости полета. Вероятно, что при правильно выбранной конфигурации винтов и системе автоматического управления можно парировать всплески тяги так называемой подборкой «фазировки» – выбором начального угла по азимуту для каждого винта

Gyrodactylus salinae n. sp. (Platyhelminthes: Monogenea) infecting the south European toothcarp Aphanius fasciatus (Valenciennes) (Teleostei, Cyprinodontidae) from a hypersaline environment in Italy

Background: Historically, non-native species of Gambusia (Poeciliidae) have been used to control larval stages of the Asian tiger mosquito, Stegomyia albopicta Reinert, Harbach et Kitching, 2004 throughout Italy. The potential utility of indigenous populations of Aphanius fasciatus (Valenciennes) (Teleostei: Cyprinodontidae) as an appropriate alternative biological control is currently being explored. A sub-sample of ten fish collected from Cervia Saline, Italy (salinity 65 ppt; 30°C) to assess their reproductive capability in captivity, harboured a moderate infection of Gyrodactylus von Nordmann, 1832 (Platyhelminthes, Monogenea). A subsequent morphological and molecular study identified this as being a new species. Results: Gyrodactylus salinae n. sp. is described from the skin, fins and gills of A. fasciatus. Light and scanning electron microscopical (SEM) examination of the opisthaptoral armature and their comparison with all other recorded species suggested morphological similarities to Gyrodactylus rugiensoides Huyse et Volckaert, 2002 from Pomatoschistus minutus (Pallas). Features of the ventral bar, however, permit its discrimination from G. rugiensoides. Sequencing of the nuclear ribosomal DNA internal transcribed spacers 1 and 2 and the 5.8S rRNA gene and a comparison with all species listed in GenBank confirmed they are unique and represent a new species (most similar to Gyrodactylus anguillae Ergens, 1960, 8.3% pair-wise distance based on 5.8S+ITS2). This represents the first species of Gyrodactylus to be described from Aphanius and, to date, has the longest ITS1 (774 bp) sequenced from any Gyrodactylus. Additional sampling of Cervia Saline throughout the year, found G. salinae n. sp. to persist in conditions ranging from 35 ppt and 5°C in December to 65 ppt and 30°C in July, while in captivity a low level of infection was present, even in freshwater conditions (0 ppt). Conclusions: The ability of G. salinae n. sp. to tolerate a wide range of salinities and temperatures shows its potential to readily adapt to several environmental conditions. These findings, together with the fact that A. fasciatus is a protected species and is considered as a biological control organism, necessitate further studies on the ecology and virulence of G. salinae n. sp

Life Cycle Replacement by Gene Introduction under an Allee Effect in Periodical Cicadas

Periodical cicadas (Magicicada spp.) in the USA are divided into three species groups (-decim, -cassini, -decula) of similar but distinct morphology and behavior. Each group contains at least one species with a 17-year life cycle and one with a 13-year cycle; each species is most closely related to one with the other cycle. One explanation for the apparent polyphyly of 13- and 17-year life cycles is that populations switch between the two cycles. Using a numerical model, we test the general feasibility of life cycle switching by the introduction of alleles for one cycle into populations of the other cycle. Our results suggest that fitness reductions at low population densities of mating individuals (the Allee effect) could play a role in life cycle switching. In our model, if the 13-year cycle is genetically dominant, a 17-year cycle population will switch to a 13-year cycle given the introduction of a few 13-year cycle alleles under a moderate Allee effect. We also show that under a weak Allee effect, different year-classes (“broods”) with 17-year life cycles can be generated. Remarkably, the outcomes of our models depend only on the dominance relationships of the cycle alleles, irrespective of any fitness advantages

Development and Testing of the Method for the Detection of Lassa virus RNA, Based on real-Time Polymerase Chain reaction with reverse Transcription

Abstract. Objective of the study was the development of a method for the detection and quantitative analysis (realtime RT-PCR) to identify genetic markers of Lassa virus - LASV-Fl. Materials and methods. We utilized all the available in the GenBank database (https://www.ncbi.nlm.nih.gov/genbank/) Lassa virus sequences that have been aligned to identify conservative sites applying the BioEdit 7.2.5 software package (IbisBiosciences, USA). To test the developed PCR kit, the control panel of Lassa virus RNA and pseudo-viral particles, 27 viral strains belonging to different fami­lies, as well as 37 serum samples from patients with feverish diseases selected in medical institutions of the Republic of Guinea in 2016-2018 and 55 samples of organ suspensions from multi-spiked mice were used. Results and discussion. The analytical sensitivity of the method varied from 103 copies/ml to 105 copies/ml and had 96.4 % diagnostic sensitivity, while the analytical and diagnostic specificity was 100 %. It is shown that the developed technique can be successfully introduced into practice for the detection of Lassa virus in the Republic of Guinea, using various types of material from small mammals, including whole blood and organ suspensions of M. natalensis, as well as samples of human blood sera collected 3-7 days after the onset of the disease. It is also suggested that this method can be used for strains of Lassa virus, common not only in Guinea but also in other endemic areas, but this fact must be confirmed in further studies

Post-COVID immunopatology syndrome: characteristics of phenotypical changes in the immune system in post-COVID patients

This study examines the long-term effects of SARS-CoV-2 infection on immune status. Given the prolonged and profound immune dysregulation observed during acute SARS-CoV-2 infection, it remains to be determined whether these changes translate into subsequent immune system dysfunction in recovering individuals. In this sense, the aim of the study was to study the parameters of the immune system in patients who had undergone SARS-CoV-2 infection. 150 patients who underwent SARS-CoV-2 infection were examined according to 96 parameters using flow cytometry. A complete blood count was performed using a Medonic device (Sweden); ELISA method determined the levels of general and specific IgM, IgG, IgA, compliment fragments (JSC Vector-Best, Russia). The activity of the phagocytes was studied according to the generally accepted method. The study found that at least four phenotypes of immune system disorders are detected in patients. The first two phenotypes are related to the impairment of innate immune system factors and are associated with a decrease in the number of CD46+ and NK cells. It has been observed that a decrease in CD46+ persists for a long time in a significant number of recovered patients, highlighted by the impaired expression of this marker in various subpopulations of lymphocytes. The decrease in the level of natural killers was accompanied by a compensatory increase in the number of T lymphocytes, mainly due to T helpers and TNK lymphocytes, and the growth of total memory B cells. Two other identified phenotypes are characterized by damage to acquired immune response factors and are associated with damage to B cells and T cytotoxic cells. The relationship of such disorders with damage to hematopoiesis erythrocyte and platelet sprouts, which contribute to the appearance of hypoxia and possible violation of the blood coagulation system, has been shown. Therefore, the results obtained indicate a long-term pronounced damage to the immune system in postCOVID patients that requires immunocorrection of these disorders