Exercise and Polycystic Ovary Syndrome.

Polycystic ovary syndrome (PCOS) is a complex endocrinopathy affecting both the metabolism and reproductive system of women of reproductive age. Prevalence ranges from 6.1-19.9% depending on the criteria used to give a diagnosis. PCOS accounts for approximately 80% of women with anovulatory infer-tility, and causes disruption at various stages of the reproductive axis. Evidence suggests lifestyle modification should be the first line of therapy for women with PCOS. Several studies have examined the impact of exercise interventions on reproductive function, with results indicating improvements in menstrual and/or ovulation frequency following exercise. Enhanced insulin sensitivity underpins the mechanisms of how exercise restores reproductive function. Women with PCOS typically have a cluster of metabolic abnormalities that are risk factors for CVD. There is irrefutable evidence that exercise mitigates CVD risk factors in women with PCOS. The mechanism by which exercise improves many CVD risk factors is again associated with improved insulin sensitivity and decreased hyperinsulinemia. In addition to cardiometabolic and reproductive complications, PCOS has been associated with an increased prevalence of mental health disorders. Exercise improves psychological well-being in women with PCOS, dependent on certain physiological factors. An optimal dose-response relationship to exercise in PCOS may not be feasible because of the highly individualised characteristics of the disorder. Guidelines for PCOS suggest at least 150 min of physical activity per week. Evidence confirms that this should form the basis of any clinician or healthcare professional prescription

Assessing the Quality of the Parent–Child Relationship: Validity and Reliability of the Child–Parent Relationship Test (ChiP-C)

The ChiP-C is a clinically oriented questionnaire for assessing the quality of the child-parent relationship according to the child’s subjective appraisal. The ChiP-C is based on family systems theory and a cumulative vulnerability model. The questionnaire consists of 36 items representing three resource scales, five risk scales, and one additional scale. This article presents the theoretical framework and main psychometric properties of the ChiP-C. A school-based sample of 1,377 youth (ages 10–20; M = 14.4) and a clinic-referred consecutive sample of 197 patients (ages 10–18, M = 14.0) were surveyed. Construct validity was determined by confirmatory factor analyses. The mean of the internal consistencies was 0.79. Systematic correlations between the ChiP-C scales and the German EMBU confirmed the convergent and discriminant validity of the ChiP-C. Moreover, all ChiP-C scales were shown to be significantly correlated with psychopathological symptoms as measured by parent and youth questionnaires. The ChiP-C can be considered an economical screening instrument for a reliable and valid assessment of strengths and disturbances of the child-parent relationship according to the child’s subjective appraisal

Peptide Nucleic Acids for MicroRNA Targeting

The involvement of microRNAs in human pathologies is firmly established. Accordingly, the pharmacological modulation of microRNA activity appears to be a very interesting approach in the development of new types of drugs (miRNA therapeutics). One important research area is the possible development of miRNA therapeutics in the field of rare diseases. In this respect, appealing molecules are based on peptide nucleic acids (PNAs), displaying, in their first description, a pseudo-peptide backbone composed of N-(2-aminoethyl)glycine units, and found to be excellent candidates for antisense and antigene therapies. The aim of the present article is to describe methods for determining the activity of PNAs designed to target microRNAs involved in cystic fibrosis, using as model system miR-145-5p and its target cystic fibrosis transmembrane conductance regulator (CFTR) mRNA. The methods employed to study the effects of PNAs targeting miR-145-5p are presented here by discussing data obtained using as cellular model system the human lung epithelial Calu-3 cell line