Mapping of mosquito breeding sites in malaria endemic areas in Pos Lenjang, Kuala Lipis, Pahang, Malaysia

<p>Abstract</p> <p>Background</p> <p>The application of the Geographic Information Systems (GIS) to the study of vector transmitted diseases considerably improves the management of the information obtained from the field survey and facilitates the study of the distribution patterns of the vector species.</p> <p>Methods</p> <p>As part of a study to assess remote sensing data as a tool for vector mapping, geographical features like rivers, small streams, forest, roads and residential area were digitized from the satellite images and overlaid with entomological data. Map of larval breeding habitats distribution and map of malaria transmission risk area were developed using a combination of field data, satellite image analysis and GIS technique. All digital data in the GIS were displayed in the WGS 1984 coordinate system. Six occasions of larval surveillance were also conducted to determine the species of mosquitoes, their characteristics and the abundance of habitats.</p> <p>Results</p> <p>Larval survey studies showed that anopheline and culicine larvae were collected and mapped from 79 and 67 breeding sites respectively. Breeding habitats were located at 100-400 m from human settlement. Map of villages with 400 m buffer zone visualizes that more than 80% of <it>Anopheles maculatus s.s</it>. immature habitats were found within the buffer zone.</p> <p>Conclusions</p> <p>This study amplifies the need for a broadening of the GIS approach which is emphasized with the aim of rejuvenating the dynamic aspect of entomological studies in Malaysia. In fact, the use of such basic GIS platforms promote a more rational basis for strategic planning and management in the control of endemic diseases at the national level.</p

Diversity of anopheline species and their <it>Plasmodium</it> infection status in rural Bandarban, Bangladesh

<p>Abstract</p> <p>Background</p> <p>Historically, the Chittagong Hill Tracts (CHT) of Bangladesh was considered hyperendemic for malaria. To better understand the contemporary malaria epidemiology and to develop new and innovative control strategies, comprehensive epidemiologic studies are ongoing in two endemic unions of Bandarban district of CHT. Within these studies entomological surveillance has been undertaken to study the role of the existing anopheline species involved in the malaria transmission cycle throughout the year.</p> <p>Methods</p> <p>CDC miniature light traps were deployed to collect anopheline mosquitoes from the sleeping room of the selected houses each month in a single union (Kuhalong). Molecular identification was carried out for available <it>Anopheles</it> species complexes. Circumsporozoite proteins (CSP) for <it>Plasmodium falciparum</it>, <it>Plasmodium vivax</it>-210 (Pv-210) and <it>Plasmodium vivax</it>-247(Pv-247) were detected by Enzyme-linked immunosorbent assay (ELISA) from the female anopheline mosquitoes. To confirm CSP-ELISA results, polymerase chain reaction (PCR) was also performed.</p> <p>Results</p> <p>A total of 2,837 anopheline mosquitoes, of which 2,576 were female, belonging to 20 species were collected from July 2009 -June 2010. <it>Anopheles jeyporiensis</it> was the most abundant species (18.9%), followed by <it>An. vagus</it> (16.8%) and <it>An. kochi</it> (14.4%). ELISA was performed on 2,467 female mosquitoes of 19 species. 15 (0.6%) female anophelines belonging to eight species were found to be positive for <it>Plasmodium</it> infection by CSP-ELISA. Of those, 11 (0.4%) mosquitoes were positive for <it>P. falciparum</it> and four (0.2%) for Pv-210. No mosquito was found positive for Pv-247. <it>An. maculatus</it> (2.1%, 2/97) had the highest infection rate followed by <it>An. umbrosus</it> (1.7%, 2/115) and <it>An. barbirostris</it> (1.1%, 2/186). Other infected species were <it>An. nigerrimus</it>, <it>An. nivipes, An. jeyporiensis</it>, <it>An. kochi</it>, and <it>An. vagus</it>. Out of 11 <it>P. falciparum</it> CSP positive samples, seven turned out to be positive by PCR. None of the samples positive for Pv-210 was positive by PCR. In terms of abundance and incrimination, the results suggest that <it>An. maculatus</it>, <it>An. jeyporiensis</it> and <it>An. nivipes</it> play important roles in malaria transmission in Kuhalong.</p> <p>Conclusion</p> <p>The findings of this study suggest that even in the presence of an insecticide impregnated bed-net intervention, a number of <it>Anopheles</it> species still play a role in the transmission of malaria. Further investigations are required to reveal the detailed biology and insecticide resistance patterns of the vector mosquito species in endemic areas in Bangladesh in order to assist with the planning and implementation of improved malaria control strategies.</p