Thymosin β10 Expression Driven by the Human TERT Promoter Induces Ovarian Cancer-Specific Apoptosis through ROS Production

Thymosin β10 (Tβ10) regulates actin dynamics as a cytoplasm G-actin sequestering protein. Previously, we have shown that Tβ10 diminishes tumor growth, angiogenesis, and proliferation by disrupting actin and by inhibiting Ras. However, little is known about its mechanism of action and biological function. In the present study, we establish a new gene therapy model using a genetically modified adenovirus, referred to as Ad.TERT.Tβ10, that can overexpress the Tβ10 gene in cancer cells. This was accomplished by replacing the native Tβ10 gene promoter with the human TERT promoter in Ad.TERT.Tβ10. We investigated the cancer suppression activity of Tβ10 and found that Ad.TERT.Tβ10 strikingly induced cancer-specific expression of Tβ10 as well as apoptosis in a co-culture model of human primary ovarian cancer cells and normal fibroblasts. Additionally, Ad.TERT.Tβ10 decreased mitochondrial membrane potential and increased reactive oxygen species (ROS) production. These effects were amplified by co-treatment with anticancer drugs, such as paclitaxel and cisplatin. These findings indicate that the rise in ROS production due to actin disruption by Tβ10 overexpression increases apoptosis of human ovarian cancer cells. Indeed, the cancer-specific overexpression of Tβ10 by Ad.TERT.Tβ10 could be a valuable anti-cancer therapeutic for the treatment of ovarian cancer without toxicity to normal cells

State : its historic role / Peter Kropotkin.

Electronic reproduction. Canberra, A.C.T. : National Library of Australia, 2010

Wi-Fi based Positioning system

Real Time Location-estimation systems (RTLS) developed for outdoor environments have shown significant errors due to changes in atmospheric conditions, multipath propagation and inherent signal strength variations. In this paper, we present an improved Wi-Fi based RTLS solution for the outdoors that reduces the above impacts. In addition this paper presents the development of a portable device for implementing the above RTLS solution. The research makes use of Euclidean, standard deviation based and triartgulation algorithms. Data smoothening is done to reduce the multipath propagation issues. The different algorithm combinations are compared via their error cumulative distribution functions

Arkhiv kni︠a︡zi︠a︡ Vi︠a︡zemskago. Kni︠a︡zʹ Andreĭ Ivanovich Vi︠a︡zemskīĭ.

Mode of access: Internet

The effect of apol i popr otei n(a)-, apol i popr otei n E-, and apol i popr otei n A 4-pol ymor phi sms on quanti tati ve l i popr otei n(a) concentr ati ons

The effects of apol i popr otei n (a), apol i popr otei n-E, and apol i popr otei n-A 4 i sofor ms on quanti tati ve l i popr otei n(a) [Lp(a)] l evel s w er e assessed i n a sampl e of 142 Dutch fami l i es consi sti ng of tw o parents and their adolescent twin offspring. A total heritability of 95% w as esti mated for pl asma Lp(a) concentr ati ons. The l ar gest par t of thi s her i tability was due to the apo(a) l ocus w hi ch expl ai ned 61% of the total var i ance i n Lp(a) l evel s. The patter n of familial correlations for the r esi dual par t of the Lp(a) var i ance that coul d not be attr i buted to the apo(a) i sofor ms, suggested geneti c i nfl uences on the r esi dual var i ance. We addr essed the questi on w hether thi s r esi dual geneti c var i ance coul d be ascr i bed to the apoE or the apoA 4 l ocus. A si mul taneous anal ysi s of al l thr ee l oci show ed that both the apoE and the apoA 4 pol ymor phi sm di d not contr i bute si gni fi cantl y to Lp(a) var i ati on. Twin Research (2000) 3, 152-158