12 research outputs found
In-column “click” preparation of hydrophobic organic monolithic stationary phases for protein separation
Affinity monolith chromatography: a review of principles and recent analytical applications
A neutral polyacrylate copolymer coating for surface modification of thiol-ene microchannels for improved performance of protein separation by microchip electrophoresis
Modelling of interfacial mass transfer in microfluidic solvent extraction: part I. Heterogenous transport
Cryogels with Affinity Ligands as Tools in Protein Purification
Affinity chromatography is one of the well-known separation techniques especially if high purity is desired. Introducing ligands on monolithic structure gives the possibility for purifying complex media such as plasma and crude extract. This chapter is focusing on the preparation of cryogels as monolithic column and immobilization of concanavalin A on its surface as ligand for capturing the glycoprotein horseradish peroxidase