The effects of Vitreous Humor on Mesenchymal Stem Cells Derived from Bone Marrow to Lens Fiber Like Cells

Introduction & Objective: The function and transparency of the lens actually diminishes with aging as a result, external intervention is necessary to amend it. The aim of the present study was to investigate the effects of vitreous humor on the mesenchymal stem cells (MSCs) to lens fiber like cells. Materials & Methods: The present experimental study was conducted at Tarbiat Moallem University in 2010. Bone marrow was collected from tibias in NMRI mice and then cultured. Immuno-cytochemistery was done to confirm the mesenchymal stem cells using Oct4 antibody. Subsequently, MSCs were treated with bovine vitreous body for 7 and 21 days. The morphology of the MSCs to the lens fibers were studied using Phase Contrast Microscope. Results: During the primary culture, the cell population was heterogeneous and in the subsequent passages, the number of the spindle-shaped cells increased. Immuno-cytochemistery study confirmed the MSCs. Morphological studies showed that most cells in the experimental group were locally longer and more aligned in parallel compared to control group cells. Moreover, lens fiber like with large nuclei and multiple nucleoli was observed. Furthermore, the concentration of 25% of vitreous body had more induction effect on MSCs in comparison with the 50% concentration. Conclusion: The MSCs derived from mouse bone marrow could differentiate into lens fiber like cells by treating them with vitreous humor

Surface Markers Expression in the Mesenchymal Stem Cells Derived from Bone Marrow

Background and aim: Mesenchymal stem cells were separated from bone marrow by Friedenstein et al. for the first time. In addition to their cohesion, mesenchymal properties of the cells were proven by surface markers. The aim of this study was to isolate mesenchymal stem cells from mouse bone marrow cultures and some surface markers in the obtained cells were expressed. Methods: In this study, bone marrow cells in the femur and tibia bones were isolated from NMRI mice. Bone marrow mesenchymal stem cells were isolated to the bottom of culture dish based on cohesion properties. In addition to their cohesion, mesenchymal properties of the cells were proven by CD73, CD44, CD90, CD31, CD45 and CD29 markers, after the fourth passage using flow cytometry. Results: In the primary culture, a heterogenous population of flattened, spindle and multi-faceted cells were observed. Spindle-shaped cells increased in later passages, so that almost all of third passage cells were spindle-shaped. Flow cytometry analysis of surface markers revealed high level mesenchymal stem cells from the fourth passage markers CD44, CD73 and CD29, and, low level of CD31, CD45 and CD90 markers. Conclusion: It can be concluded that MSCs derived from bone marrow mouse have some positive and negative surface markers with high and low expression, respectively. The expression rate of these markers proves the mesenchymal properties of cells. Keyword: Bone Marrow, Mesenchymal Stem Cells, Surface Marker