Quantitative Histomorphometry of the Healthy Peritoneum

The peritoneum plays an essential role in preventing abdominal frictions and adhesions and can be utilized as a dialysis membrane. Its physiological ultrastructure, however, has not yet been studied systematically. 106 standardized peritoneal and 69 omental specimens were obtained from 107 patients (0.1-60 years) undergoing surgery for disease not affecting the peritoneum for automated quantitative histomorphometry and immunohistochemistry. The mesothelial cell layer morphology and protein expression pattern is similar across all age groups. Infants below one year have a thinner submesothelium; inflammation, profibrotic activity and mesothelial cell translocation is largely absent in all age groups. Peritoneal blood capillaries, lymphatics and nerve fibers locate in three distinct submesothelial layers. Blood vessel density and endothelial surface area follow a U-shaped curve with highest values in infants below one year and lowest values in children aged 7-12 years. Lymphatic vessel density is much lower, and again highest in infants. Omental blood capillary density correlates with parietal peritoneal findings, whereas only few lymphatic vessels are present. The healthy peritoneum exhibits major thus far unknown particularities, pertaining to functionally relevant structures, and subject to substantial changes with age. The reference ranges established here provide a framework for future histomorphometric analyses and peritoneal transport modeling approaches. © 2016, EDP Science. All rights reserved

Non-intrusive temperature measurement using microscale visualization techniques

mu PIV is a widely accepted tool for making accurate measurements in microscale flows. The particles that are used to seed the flow, due to their small size, undergo Brownian motion which adds a random noise component to the measurements. Brownian motion introduces an undesirable error in the velocity measurements, but also contains valuable temperature information. A PIV algorithm which detects both the location and broadening of the correlation peak can measure velocity as well as temperature simultaneously using the same set of images. The approach presented in this work eliminates the use of the calibration constant used in the literature (Hohreiter et al. in Meas Sci Technol 13(7):1072-1078, 2002), making the method system-independent, and reducing the uncertainty involved in the technique. The temperature in a stationary fluid was experimentally measured using this technique and compared to that obtained using the particle tracking thermometry method and a novel method, low image density PIV. The method of cross-correlation PIV was modified to measure the temperature of a moving fluid. A standard epi-fluorescence mu PIV system was used for all the measurements. The experiments were conducted using spherical fluorescent polystyrene-latex particles suspended in water. Temperatures ranging from 20 to 80A degrees C were measured. This method allows simultaneous non-intrusive temperature and velocity measurements in integrated cooling systems and lab-on-a-chip devices

Laser diagnostics of pulverized coal combustion in O2/N2 and O2/CO2 conditions: velocity and scalar field measurements

Optical diagnostic techniques are applied to a 21 kW laboratory-scale pulverized coal–methane burner to map the reaction zone during combustion, in mixtures with varying fractions of O2, N2 and CO2. Simultaneous Mie scatter and OH planar laser-induced fluorescence (PLIF) measurements have been carried out to study the effect of the oxidizer/diluent concentrations as well as the coal-loading rate. The spatial distribution of soot is captured using laser-induced incandescence (LII). Additionally, velocity profiles at selected axial locations are measured using the pairwise two-dimensional laser Doppler velocimetry technique. The OH PLIF images capture the reaction zones of pilot methane–air flames and the variation of the coal flame structure under various O2/CO2 compositions. Coal particles devolatilize immediately upon crossing the flame interface, so that the Mie scatter signal almost vanishes. Increasing coal-loading rates leads to higher reaction rates and shorter flames. LII measurements show that soot is formed primarily in the wake of remaining coal particles in the product regions. Finally, differences in the mean and RMS velocity field are explained by the combined action of thermal expansion and the changes in particle diameter between reacting and non-reacting flows.Engineering and Physical Sciences Research Counci