13 research outputs found
Studies on the production of nigerloxin using agro-industrial residues by solid-state fermentation
Straightforward ladder sequencing of peptides using a Lys-N metalloendopeptidase.
We introduce a method for sequencing peptides by mass spectrometry using a metalloendopeptidase that cleaves proteins at the amino side of lysine (Lys-N). When analyzed by electron transfer dissociation (ETD)-based mass spectrometric sequencing, Lys-N-digested peptides that contain a single lysine residue produce spectra dominated by c-type fragment ions, providing simple ladders for sequence determination. This method should be a valuable strategy for de novo sequencing and the analysis of post-translational modifications
Influence functions of two families of robust estimators under proportional scatter matrices
Robust estimation, Robust scatter matrices, Projection-pursuit, Proportional scatter models, Partial influence curves,