Establishing Campylobacter culture methods in a clinical diagnostic laboratory and the first report of Campylobacter species isolation in northern Sri Lanka

Introduction: The Enteric Reference Laboratory of the Medical Research Institute (ERL/MRI), Colombo is the only public sector laboratory in Sri Lanka that performs Campylobacter cultures. Due to logistic limitations involving specimen transport from distant sites, efforts were taken to establish Campylobacter culture facilities in our local clinical microbiology laboratory.Methods: A blood-free charcoal-based selective agar medium (Karmali medium) was chosen based on performance characteristics and quality control (QC)/verification performed at the ERL/MRI. A suitable incubating method was assessed and chosen, and QC was performed in our laboratory. A technical staff member of our local laboratory received capacity building training at the ERL/MRI.Results: The quality control/verification process of the Karmali medium was satisfactory. The variable atmospheric incubator was chosen as the incubating method as it was shown to be more economical in the long-term given the anticipated work load and the QC was satisfactory. Following a satisfactory verification process, Campylobacter culture method was introduced in our laboratory. Five C. jejuni and one hippurate-negative C. jejuni/C. coli was detected in faecal specimens of six paediatric patients between May-December 2018. The isolation rate was 2.25% (6/267). Ciprofloxacin resistance was detected in four out of five C. jejuni isolates.Conclusion: Establishing Campylobacter culture methods in a routine clinical diagnostic laboratory will be beneficial in regions with high prevalence of diarrhoeal disease and with logistic limitations for specimen transport to the central reference laboratory. This is the first report of isolation and antimicrobial susceptibility of Campylobacter species from patients in northern Sri Lanka.</p

Fuzzy based stabilizer control system for quad-rotor

In this paper the design, development and testing of a stabilizer control system for a Quad-rotor is presented which is focused on the maneuverability. The mechanical design is performed along with the design of the controlling algorithm which is devised using fuzzy logic controller. The inputs for the system are the angular positions and angular rates of the Quad-rotor relative to three axes. Then the output data is filtered from an accelerometer and a gyroscope through a Kalman filter. In the development of the stability controlling system Mandani fuzzy model is incorporated. The results prove that the fuzzy based stabilizer control system is superior in high dynamic disturbances compared to the traditional systems which use PID integrated stabilizer control systems

Harvesting of kinetic energy of the raindrops

This paper presents a methodology to harvest the kinetic energy of the raindrops using piezoelectric devices. In the study 1m×1m PVDF (Polyvinylidene fluoride) piezoelectric membrane, which is fixed by the four edges, is considered for the numerical simulation on deformation of the membrane due to the impact of the raindrops. Then according to the drop size of the rain, the simulation is performed classifying the rainfall types into three categories as light stratiform rain, moderate stratiform rain and heavy thundershower. The impact force of the raindrop is dependent on the terminal velocity of the raindrop, which is a function of diameter. The results were then analyzed to calculate the harvestable energy from the deformation of the piezoelectric membrane