Geochronological study of post-metamorphic granite from Kasumi Rock, Lutzow-Holm Complex, East Antarctica

Kasumi Rock is situated in the amphibolite-facies metamorphic zone in the Lutzow-Holm Complex, East Antarctica. In this area, granitic rocks occur as steeply dipping linear dykes with sharp intrusive contacts, and cross-cut the foliation of layered metamorphic rocks. A geochronological study of this post-metamorphic granite (PMG) has been performed. An Rb-Sr whole rock isochron for five granitic rocks defines an age of 492.1+-23.4 Ma with an initial ^87Sr/^86Sr ratio of 0.70535+-0.00027 (MSWD=0.08). In the Sm-Nd isochron diagram, four rock samples yield an age of 498.4+-90.6 Ma with an initial ^143Nd/^144Nd ratio of 0.511782+-0.000101 (MSWD=0.05). The whole rock isochron ages and mode of occurrence suggest that the Kasumi PMGs were intruded after regional cooling of the area, and probably affected local reequilibrium for isotopic systems of surrounding metamorphic rocks. The PMGs might have been slowly cooled and crystallized after the intrusion. The Kasumi PMGs have no genetic relation to the Oku-iwa PMGs as regards Rb-Sr and Sm-Nd isotopic systems, because the initial ratios of both PMGs are quite different from each other. This is interpreted to mean that the PMGs in the Lutzow-Holm Complex were originated from different source materials or had different chemical reaction processes with crustal materials

A case of milk allergy that presented anaphylaxis after cutaneous contact with allergen

ABSTRACTMilk allergy in a 1-year and 8-month-old boy is reported. At 1 year and 1 month of age, the patient presented with anaphylaxis, including erythema, which was initially localized to the contact site of the anterior chest, and wheezing accompanied by dyspnea, 5 min after contact with milk allergen through his atopic skin. These symptoms continued for 50 min. Seventy minutes after the disappearance of the initial erythema, the patient developed subsequent erythematous lesions distributed throughout the neck and head area that persisted for as long as 24 h. On another occasion, he also exhibited a pale face and generalized erythema immediately after an accidental oral ingestion of milk at the age of 1 year and 8 months. He had been unsettled for several hours when an intravenous steroid was administered. His serum IgE was 590 IU/mL and the radioallergosorbent test (RAST) scores against milk, α-lactoalbumin, β-lactoglobulin, casein and cheese were 5, 2, 3, 5 and 5, respectively. This is a rare case of a patient with milk allergy who fell into anaphylaxis following both cutaneous contact with and oral ingestion of the offending milk protein. Care should be taken with patients with food allergies because cutaneous contact with the offending food may cause adverse reactions, including anaphylaxis

Effect of Lubrication and Forging Load on Surface Roughness, Residual Stress, and Deformation of Cold Forging Tools

Cold forging is a metal forming that which uses localized compressive force at room temperature. During the cold forging process, the tool is subjected to extremely high loads and abrasive wear. Lubrication plays an important role in cold forging to improve product quality and tool life by preventing direct metallic contact. Surface roughness and residual stress also greatly affects the service life of a tool. In this study, variations in surface roughness, residual stress, and specimen deformation with the number of cold forging cycles were investigated under different forging conditions. Specimens that were made of heat-treated SKH51 (59-61 HRC), a high-speed tool steel with a polished working surface, were used. The specimens were subjected to an upsetting process. Compressive residual stress, surface roughness, and specimen deformation showed a positive relationship with the number of forging cycles up to a certain limit and became almost constant in most of the forging conditions. A larger change in residual stress and surface roughness was observed at the center of the specimens in all the forging conditions. The effect of the magnitude of the forging load on the above discussed parameters is large when compared to the effect of the lubrication conditions

Guideline-based Treatment of Glucocorticoid-induced Osteoporosis in Patients with Rheumatoid Arthritis: A Retrospective Study with the AORA Registry

Glucocorticoid-induced osteoporosis (GIOP) is one of the side effects associated with glucocorticoid (GC) therapy. In 2014, the Japanese Society for Bone and Mineral Research (JSBMR) provided new guidelines for the management and treatment of GIOP. The aim of the present study was to clarify the prevalence of patients with rheumatoid arthritis (RA) requiring treatment according to the new guidelines and to identify risk factors associated with lack of treatment in these patients. Patients in the 2018 Akita Orthopedic group on Rheumatoid Arthritis (AORA) database were enrolled. Of 2,234 patients with RA in the database, 683 (30.6%) met the 2014 JSBMR guideline treatment criteria, and 480 (70.3%) had been treated. The untreated group included a larger number of males, younger patients, and patients treated in clinics rather than hospital (p<0.001, p=0.015, and p<0.001, respectively). Multivariate analyses found that male sex, younger age, and clinic-based RA care were significant risk factors associated with lack of treatment (p<0.001, p=0.013, and p<0.001, respectively). Thus, male sex, younger age, and clinic-based care were identified as risk factor

An Integrated Approach to Developing Japanese Students' English Presentation and Discussion Skills

We examined the types of difficulties that Japanese high school students have in English presentation and discussion activities. We conducted factor analysis (principal factor method with promax rotation) on the survey data, which was divided into four stages: (1) the presentation preparation stage; (2) the presentation stage; (3) the listening stage; and (4) the discussion stage. The results showed that students had difficulty with the following skills during each of the four stages: (1) "effective ways of providing information," "revision," and "collaborative work" during the presentation preparation stage; (2) "basic presentation skills," "handling questions," and "audience management" during the presentation stage ; (3) "active interaction" and "active understanding" during the listening stage; and (4) "overcoming communication anxiety," "adaptation to classroom activities," "problem solving approach," and "self-relativization" during the discussion stage

An Integrated Approach to Developing Japanese Students' English Presentation and Discussion Skills

We examined the types of difficulties that Japanese high school students have in English presentation and discussion activities. We conducted factor analysis (principal factor method with promax rotation) on the survey data, which was divided into four stages: (1) the presentation preparation stage; (2) the presentation stage; (3) the listening stage; and (4) the discussion stage. The results showed that students had difficulty with the following skills during each of the four stages: (1) "effective ways of providing information," "revision," and "collaborative work" during the presentation preparation stage; (2) "basic presentation skills," "handling questions," and "audience management" during the presentation stage ; (3) "active interaction" and "active understanding' during the listening stage; and (4) "overcoming communication anxiety," "adaptation to classroom activities," "problem solving approach," and "self-relativization" during the discussion stage

An Anti-apoptotic Role of NF-κB in TNFα-induced Apoptosis in an Ameloblastoma Cell Line

AbstractNuclear factor-κB (NF-κB) is involved in the promotion of cell survival in a variety of cell types. The present study focused on the role of NF-κB in TNFα-induced apoptosis in an ameloblastoma. Immunohistochemical staining revealed p65 NF-κB protein to be expressed in ameloblastoma tissues. Furthermore, immunoblotting and immunocytochemistry analyses showed that the stimulation of TNFα in an ameloblastoma cell line (AM-1) induced p65 NF-κB translocation from the cytoplasm to the nucleus, indicating NF-κB activation. These findings were confirmed by an NF-κB luciferase reporter assay, which detected enhanced NF-κB transcription activity of AM-1 cells by TNFα stimulation. Moreover, pretreatment with SN50, a nuclear translocation inhibitor, prior to TNFα stimulation, effectively inhibited TNFα-induced NF-κB activation in AM-1 cells. In order to reveal the role of NF-κB activation during TNFα-induced apoptosis in AM-1 cells, an apoptosis assay was performed, and showed that the potential of TNFα in inducing apoptosis in AM-1 cells was significantly elevated by inhibiting the NF-κB activation. These results suggest that NF-κB plays an anti-apoptotic role in TNFα-induced apoptosis in AM-1 cells

Apoptotic function of tumor-associated antigen RCAS1 in oral squamous cell carcinoma

BACKGROUND: Receptor-binding cancer antigen expressed on SiSo cell (RCAS1) is derived from uterine adenocarcinoma and can induce apoptosis in lymphocytes, allowing tumor cells to escape from immune surveillance. RCAS1 is reportedly expressed in a membranous pattern on tumor cell or soluble one in serum of patients. The aim of this study was to investigate expression patterns of RCAS1 and the effect on apoptosis in oral squamous cell carcinoma (OSCC) cell lines. METHODS: In four kinds of OSCC cell lines (HSC-2, HSC-3, SQUU-A, and SQUU-B), RCAS1 mRNAs and proteins were determined by RT-PCR and immunocytochemistry. Membranous RCAS1 was determined by flow cytometry. Culture supernatants were analyzed for detection of soluble RCAS1 by dot blotting and enzyme-linked immunosorbent assay. Apoptotic ability of RCAS1 on the erythroid leukemia cell line K562 with the putative receptor was evaluated by flow cytometry in co-culture with highly metastatic SQUU-B, with knocked-down RCAS1 cells or in a no-cell contact condition. RESULTS: RCAS1 mRNA and proteins were expressed in all of OSCC cell lines. Membranous pattern were expressed in all cell lines, while soluble pattern was detected in all supernatants. RCAS1 mRNA, membranous and soluble RCAS1 were significantly seen in SQUU-B more than the other 3 cell lines (P < 0.05). K562 apoptosis was induced in co-culture with each of all cell lines, particularly with SQUU-B. Apoptosis was markedly reduced in co-culture with RCAS1 knockdown cells, but was induced in co-culture without cell contract of SQUU-B. CONLUSIONS: Our study suggests that RCAS1 has an apoptotic function via membranous/soluble expression pattern in OSCC cells. RCAS1 may thus affect tumor escape from immune surveillance in OSCC by inducing apoptosis