17 research outputs found
Rearrangement of Variable Region T Cell Receptor y Genes in Acute Lymphoblastic Leukemia Vy Gene Usage Differs in Mature and Immature T Cells
Get PDFUsing probes recognizing variable regions (V gamma) and joining regions (J gamma) of the T cell receptor (TCR) gamma gene, we have analyzed the usage of V gamma genes in 24 patients with T cell acute lymphoblastic leukemia (ALL) and 36 patients with B-precursor ALL. In CD3- T-ALL derived from immature T cells, V gamma genes more proximal to J gamma were frequently rearranged; V gamma 8, V gamma 9, V gamma 10, and V gamma 11 were used in 19 of 24 rearrangements. In contrast, CD3+ T-ALL derived from a more mature stage of T cell ontogeny, showed a high frequency of rearrangements involving V gamma genes distal to J gamma; V gamma 2, V gamma 3, V gamma 4, and V gamma 5 were used in 17 of 25 rearrangements. In B-precursor ALL, no notable bias of V gamma gene usage was observed. This probably reflects the possibility that TCR genes may not rearrange according to a T cell hierarchy when under control of a B cell gene program. Furthermore, deletions of those V gamma genes located 3' to rearranged V gamma genes were observed in all patients analyzed. This supports the theory that loop deletion is a major mechanism for TCR-gamma gene rearrangement
エイセイ ギジュツカ ダイ2ガクネン ニ オケル ビョウイン ジッシュウ ノ コウカ ニ カンスル ケントウ
Get PDF衛生技術科では,2000年(平成12年度)より2年生の夏期休暇中および春期休暇中に,関連病院であるときわ病院で5日間の病院実習を行っている。3年目を迎えることを機に,平成14年春期実習,同年夏期実習において学生にアンケート調査を実施して,この実習の効果を検討した。また,学内実習について,病院実習開始以前と病院実習開始後で実習状況を比較検討した。実習内容は手術から組織診断まで一連の過程の見学,学生相互の超音波検査・採血実習,さらに医師による診察の見学などである。これらを通して,学生は医療職として患者に対する連切な態度やチーム医療の重要性を学びとっていること,成功体験が関連教科への興味に繋がっていること等が明らかになった。また,採血を含む学内での実習が円滑に行われるようになったという効果もあった。しかし,一方で明らかになった基礎知識の不足や医療に関する社会的問題への関心の薄さは,病院実習をより充実させていくためにこれから取り組まなくてはならない課題である。今回の調査から,早期の病院実習は学生の学習意欲を喚起し,高度化する医療を担う臨床検査技師を育成するために重要な役割を果たすことが確認された。今後も総合的な学習の場として,充実させていくべきであると考える。Since 2000, the Medical Technology department has used the spring and summer vacations of second-year students for five-day practical training sessions at Tokiwa Hospital, a hospital with ties to Tokiwa College. With the 2002 term marking the third year of the program, we distributed a questionnaire for both the spring and summer sessions, investigating the effectiveness of this program. Students learned the appropriate attitude to patients as a medical staff member, as well as the importance of team health care, through a series of observations ranging from an operation to pathologic diagnosis of tissues, inter-student abdominal ultrasound examinations, inter-student blood drawing, and the observation of a doctor\u27s physical examination. It was seen that successful experiences such as these among the students aroused interest in related subjects, and an additional effect of this training was to increase the smoothness of the subsequent courses containing blood-drawing practice held in the college. On the other hand, effort to improve the effectiveness of the practical training sessions is still required in order to deal with the students\u27 lack of basic knowledge, and lack of interest in medical issues that were revealed in the current sessions. This investigation confirmed that early practical training sessions in Tokiwa Hospital serve the important functions of both increasing the students\u27 will to study, and educating medical technologists who accomplish increasingly advanced medical examinations. This training should be continued as a opportunity for systematic study in the early stage of the Medical Technology department
