5 research outputs found
Not Available
Not AvailableWhether it is better to use viable or non-viable pro bionts in aquaculture is still a matter of debate. In this
study, the molecular immunomodulation in rainbow
trout Oncorhynchus mykissinduced by viable or killed
forms of the probiont Lactobacillus rhamnosus JCM
1136 was investigated. Three forms of this probiont:
(1) heat-killed (HK), (2) live spray (LI) and (3) freeze dried (FD) were incorporated into a basal (control)
diet for rainbow trout O. mykiss. The LI and FD diets
are referred to as viable diets. A rearing trial, in tripli cate, was conducted for 30 days, with the control and
probiotic diets as treatments. The cytokine genes
such as the tumour necrosis factor (TNF), transform ing growth factor (TGF-b), interferon (IFN) and
immune gene Immunoglobulin (Ig) found in tissues
from the kidney and spleen were assessed for their
expression pattern by real-time polymerase chain re action. The tested immune genes were up-regulated
in the treatment groups, sometimes even in many
folds like in the case of the Ig gene. The TNF gene
was found to be highly (Po0.05) up-regulated
(5000-fold) in groups fed both viable forms (LI, FD).
With regard to the TGF-b gene, the spleen of the HK
and FD groups showed signi¢cant up-regulation of
20- and 30-folds respectively. The IFN gene was up regulated (Po0.05) in all treatments, but more in the
viable diet treatments. Kidney and spleen tissues
showed similar expression patterns, i.e. all of these
genes were up-regulated more with the viable diets
that with the control, and in most cases, the viable
diets induced a higher expression of the immune
genes than the HK diet.Not Availabl