Method to disperse lipids as aggregates in oil for bilayers production

Several techniques to assemble artificial lipid bilayers involve the zipping of monolayers. Theirefficiency is determined by the renewal of the saturated monolayers to be zipped and this proceeds byadsorption of lipids dispersed in oil as aggregates. The size of these lipids aggregates is a key parameterto ensure both the stability of the suspension and a fast release of lipids at the interface. We proposea new method inspired from the solvent-shifting nucleation process allowing to control and tune thelipid aggregates size and that improves the production of artificial membranes. It is simpler and fasterthan current methods starting from a dry lipid film, which are highly sensitive to environmental conditions.This method opens the route to bilayer production processes with new potentialities in membranecomposition