96 research outputs found
AnĂĄlise de Obras CinematogrĂĄficas para Compreender as ConcepçÔes de Professores sobre o Aluno com DeficiĂȘncia
Induction and transfer of resistance to poisoning by Amorimia pubiflora in sheep whith non-toxic dosis of the plant and ruminal content
Aspects of leaf anatomy of kudzu (Pueraria lobata, Leguminosae-Faboideae) related to water and energy balance
An Easy and Efficient Method for Native and Immunoreactive Echinococcus granulosus Antigen 5 Enrichment from Hydatid Cyst Fluid
Background: Currently, the serodiagnosis of cystic echinococcosis relies mostly on crude Echinococcus granulosus hydatid cyst fluid as the antigen. Consequently, available immunodiagnostic tests lack standardization of the target antigen and, in turn, this is reflected on poor sensitivity and specificity of the serological diagnosis.
Methodology/Principal Findings: Here, a chromatographic method enabling the generation of highly enriched Antigen 5 (Ag5) is described. The procedure is very easy, efficient and reproducible, since different hydatid cyst fluid (HCF) sources produced very similar chromatograms, notwithstanding the clearly evident and extreme heterogeneity of the starting material. In addition, the performance of the antigen preparation in immunological assays was preliminarily assessed by western immunoblotting and ELISA on a limited panel of cystic echinococcosis patients and healthy controls. Following western immunoblotting and ELISA experiments, a high reactivity of patient sera was seen, with unambiguous and highly specific results.
Conclusions/Significance: The methods and results reported open interesting perspectives for the development of sensitive diagnostic tools to enable the timely and unambiguous detection of cystic echinococcosis antibodies in patient sera.This work was supported by Regione Autonoma della Sardegna (http://www.regione.sardegna.it/)Pubblicat
Propriedades Ăłpticas de amostras policristalinas LiGaSiO4 contendo Fe3+ como impureza substitucional
NĂveis de feno de alfafa e forma fĂsica da ração no desempenho de cordeiros em creep feeding
Caracterização fĂsica, quĂmica e bioquĂmica do mesocarpo interno de frutos do pequizeiro colhidos em diferentes estĂĄdios de desenvolvimento
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Track A Basic Science
Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/138319/1/jia218438.pd
Synergistic potential of dillapiole-rich essential oil with synthetic pyrethroid insecticides against fall armyworm
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