Isolation, Characterization and Quantity Determination of Aristolochic Acids, Toxic Compounds in Aristolochia bracteolata L.

Background Aristolochic Acids (AAs) are major components of plants in Aristolochia and have been found to be nephrotoxic, carcinogenic and mutagenic. Herein reported are the isolation, identification and quantity determination methods of Aristolochic Acid-I (AA-I) and Aristolochic Acid-II (AA-II) toxic compounds of Aristolochia bracteolata indigenous to Central Sudan and medicinally used in diverse biological functions including analgesic and diuretic effects, treatment of tumors, malaria and/or fevers. Methods and results AAs mixture was extracted with methanol from the defatted material of Aristolochia bracteolata whole plant at room temperature and was isolated from the aqueous methanol extract by chloroform. Moreover, Silica-gel column chromatography and Preparative Thin Layer Chromatography (PTLC) using chloroform/methanol gradient mixtures were used to isolate AAs mixtures as a yellow crystalline solid. A preliminary detection of AAs was made by Thin Layer Chromatography (silica-gel, chloroform: methanol (6:1)). The Rf value of the acids mixture was 0.43-0.46. The presence of AAs in plant sample was confirmed by High Performance Liquid Chromatography/Ultraviolet (HPLC/UV) analysis using 1% acetic acid and methanol (40:60) as mobile phase and maximum absorption wave length of 250 nm. Quantitative determination of AA-II (49.03 g/kg) and AA-I (12.98 g/kg) was also achieved by HPLC/UV. Recommendation It is recommended that the use of Aristolochia bracteolata as a medicinal plant should be extremely limited or strictly prohibited. The chromatograms obtained in this study can serve as fingerprints to identify AAs in plant samples

HDR reservoir analysis incorporating acoustic emission data

A set of models of HDR systems is presented which attempts to explain the formation and operation of HDR systems using only the in-situ properties of the fractured rock mass, the earth stress field, the engineering intervention applied by way of stimulation and the relative positions and pressures of the well(s). A statistical and rock mechanics description of fractures in low permeability rocks provides the basis for modeling of stimulation, circulation and water loss in HDR systems. The model uses a large number of parameters, chiefly simple directly measurable quantities, describing the rock mass and fracture system. The effect of stimulation (raised fluid pressure allowing slip) on fracture apertures is calculated, and the volume of rock affected per volume of fluid pumped estimated. The total rock volume affected by stimulation is equated with the rock volume containing the associated AE (microseismicity). The aperture and compliance properties of the stimulated fractures are used to estimate impedance and flow within the reservoir. Fluid loss from the boundary of the stimulated volume is treated using radial leak-off with pressure-dependent permeability