43 research outputs found

    ATLAS detector and physics performance: Technical Design Report, 1

    Get PDF

    Voltammetric Detection of Damage to DNA by Arsenic Compounds at a DNA Biosensor

    Get PDF
    DNA biosensor can serve as a powerfull tool for simple in vitro tests of chemicaltoxicity. In this paper, damage to DNA attached to the surface of screen-printed carbonelectrode by arsenic compounds in solution is described. Using the Co(III) complex with1,10-phenanthroline, [Co(phen)3]3+ , as an electrochemical DNA marker and the Ru(II)complex with bipyridyne, [Ru(bipy)3]2+ , as a DNA oxidation catalyst, the portion of originaldsDNA which survives an incubation of the biosensor in the cleavage medium was evaluated.The model cleavage mixture was composed of an arsenic compound at 10-3 mol/Lconcentration corresponding to real contaminated water, 2x10-4 mol/L Fe(II) or Cu(II) ions asthe redox catalyst, and 1.5x10-2 mol/L hydrogen peroxide. DNA damage by arsenite,dimethylarsinic acid as the metabolic product of inorganic arsenic and widely used herbicide,as well as phenylarsonic acid and p-arsanilic acid as the representatives of feed additives wasfound in difference to arsenate

    Voltammetric detection of damage to DNA by arsenic compounds at a DNA biosensor

    No full text
    Abstract: DNA biosensor can serve as a powerfull tool for simple in vitro tests of chemical toxicity. In this paper, damage to DNA attached to the surface of screen-printed carbon electrode by arsenic compounds in solution is described. Using the Co(III) complex with 1,10-phenanthroline, [Co(phen)3] 3+, as an electrochemical DNA marker and the Ru(II) complex with bipyridyne, [Ru(bipy)3] 2+, as a DNA oxidation catalyst, the portion of original dsDNA which survives an incubation of the biosensor in the cleavage medium was evaluated. The model cleavage mixture was composed of an arsenic compound at 10-3 mol/L concentration corresponding to real contaminated water, 2x10-4 mol/L Fe(II) or Cu(II) ions as the redox catalyst, and 1.5x10-2 mol/L hydrogen peroxide. DNA damage by arsenite, dimethylarsinic acid as the metabolic product of inorganic arsenic and widely used herbicide, as well as phenylarsonic acid and p-arsanilic acid as the representatives of feed additives was found in difference to arsenate
    corecore