Analysis of Pollen Collected By Andrena Flavipes Panzer (Hymenoptera: Andrenidae) in Sweet Cherry Orchards, Afyonkarahisar Province of Turkey

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Effect of some Turkish propolis on the product quantity of Agaricus bisporus (Lange.) Sing

WOS: 000186105000020In this study, the effect of some Turkish propolis on the product quantity of cultivated mushroom Agaricus bisporus (Lange.) Sing. was determined. The samples of propolis were obtained from (Bursa and Erzurum) regions of Turkey. Propolis extracts were prepared as 0.5 EEP and 2.0 EEP and sprayed on compost, compost + casing soil and compost + casing soil + 1. flush of Agaricus bisporus at different times of growth period under controlled laboratory conditions Propolis showed stimulatory effects on the developmental stages and some parameters of the yield. An early yield of mush-rooms, rapid growing and increase of total weight of harvested basidiocarps were observed as compared to control without propolis in which primordium and basidiocarp formations showed great reduction. Chemical analysis of all the harvested mushroom that were cultivated on the product conditions with propolis were made by gas chromotography (GC)-mass spectrometry (MS)

Airborne Pollen Concentration in Ankara, Turkey 1990-1993

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Efficacy of propolis as an intracanal medicament against Enterococcus faecalis

PubMed ID: 17004565This study sought to compare the antibacterial efficacy of three commonly used intracanal medicaments with propolis against Enterococcus faecalis. This study utilized 180 freshly extracted single-rooted intact human permanent teeth with a single root canal. After root canal preparations and sterilization, canals were contaminated with E faecalis and incubated at 37°C (±1.0°C) for seven days. The teeth were divided randomly into six groups. To determine bacterial growth on blood agar, microbiological samples were carried out with sterile paper points to evaluate results at 48 hours and at ten days. All data were analyzed statistically with t-test, Mann Whitney, Kruskal Wallis, and one-way ANOVA tests. This study revealed that propolis had good in vitro antibacterial activity against E faecalis in the root canals, suggesting that it could be used as an alternative intracanal medicament

Propolis effect on sclerotial formations of Morchella conica Pers.

WOS: 000235315600030In this study, the effects of propolis at different concentrations on sclerotial formations of Morchella conica Pers., were examined. The propolis used was obtained from three different regions of Turkey. The propolis extracts were prepared at 0.5, 1.0, 1.5 and 2.0 EEP concentrations and they were added to malt extract agar. During the incubation period of 10 days, the mycelium of control group developed rhizomorphic and parallel oil the surface of agar medium and there was no pigmentation. On the other hand, the mycelium did not develop normally oil the agar medium in the presence of propolis but the sclerotial formations were observed. The sclerotial cells were spherical in form, with thick walls and they were characterised with different pigmentations

and histopathological approach

Background: Organophosphate (OP) insecticides are the most widely used in both agricultural and landscape pest control. The mortality and morbidity rate of OP poisoning is high. The aim of the present study is to investigate the effect of acute organophosphate exposure on hepatocyte and to examine caspase 1 and caspase-3 gene expression, and cell apoptosis related genes as p53, Tumor Necrosis Factor-alpha, Hypoxia Inducible Factor 1-alpha expression changes in rat hepatocyte.Material and Methods: 10 adult WistarAlbino female rats weighing 250-300 g were divided into control (n=5) and experiment (n=5) groups. In experimental group, rats were treated 25 mg/kg of dichlorvos (Bayer DDVP EC 550, Bayer) in corn oil by 16 gauge oral gavage tube. In control group, rats were treated only 2.5 ml corn oil by oral gavage. After seven days, all of the rats were sacrificed by cervical dislocation under anesthesia. The liver was removed and divided into fragments. Hepatocyte density and histopathological examination were performed in fixed liver tissues. For this purpose, sections were taken and stained with hematoxylin-eosin. A part of the liver was used for gene expression analysis. Total RNA was extracted from the liver tissue using an RNA isolation reagent via manufacturer's instruction. Changes in mRNA levels, detected using semi-quantitative reverse transcription polymerase chain reaction, were calculated as the proportion of the target gene amplification products to the amplification products of the housekeeping gene GAPDH.Results: Hepatocyte density were decreased in experimental group compared to control group (p<0.05). The histopathological changes, such as portal inflammation and picnosis were observed in liver sections of experimental group. According to molecular genetics analysis, Caspase 1, Caspase 3, and p53 gene expression were increased in liver tissue after dichlorvos treated rats compared with the control group. There were no expression changes for TNF-Alpha and Hifl-Alpha gene expression level among groups.Conclusion: Acute organophosphate exposure leads to loss in hepatocyte. Correlation with histopathological results, OP compound-induced cytotoxicity may be modulated through multiple sites including caspase1-3 pathway and also changes in the quantitative criteria of molecular markers of apoptosis in the rat hepatocytes on formation of behavioral skills were characterized by increased in caspase expressions in the hepatocyte

Effects of Manisa propolis on telomerase activity in leukemia cells obtained from the bone marrow of leukemia patients

WOS: 000272476400006PubMed ID: 19817639Propolis is a resinous material collected by honeybees and obtained from beehives that has anticancer effects by inducing apoptosis. The aim of this study is to investigate the effect of propolis on human telomerase reverse transcriptase (hTERT) in the leukemia cells obtained from leukemia patients. Four different bone marrow cell cultures from each of four leukemia cases were prepared. The 60 ng/ml, 30 ng/ml and 15 ng/ml working concentrations of propolis were administered to three cultures of each patient, while one culture contained only culture medium. hTERT mRNA expression levels of cells were detected at 24 h, 48 h and 72 h using the LightCycler 2.0 instrument. A significant decrease in hTERT expression levels was observed in the 60 ng/ml concentration of propolis. In conclusion, Manisa propolis may also have a potential effect on the expression of hTERT in leukemia-particularly owing to its constituent chrysin.Ege University Science and Technology CenterEge UniversityThe authors would like to thank Ege University Science and Technology Center for supporting this study

Risk of human exposure to arsenic and other toxic elements from geophagy : trace element analysis of baked clay using inductively coupled plasma spectrometry

Geophagy or earth-eating is common amongst some Bangladeshi women, especially those who are pregnant, both in Bangladesh and in the United Kingdom. A large proportion of the population in Bangladesh is already exposed to high concentrations of arsenic (As) and other toxic elements from drinking contaminated groundwater. Additional exposure to As and other toxic elements from non-food sources has not been adequately addressed and here we present the first study to monitor As levels in baked clay (known as sikor). Methods: Sikor samples originating from Bangladesh were digested using a microwave digester and analysed for their As, Pb, Cd, Mn, Fe and Zn levels using ICP-MS. Detailed As speciation analysis was performed using HPLC-ICPMS. Results: Of particular concern were the levels of As (3.8-13.1 mg kg-1), Cd (0.09-0.4 mg kg-1) and Pb (21-26.7 mg kg-1) present in the sikor samples and their possible impact on human health. Speciation analysis revealed that sikor samples contained mainly inorganic As. Modest consumption of 50 g of sikor is equivalent to ingesting 370 μg of As and 1235 μg of Pb per day, based on median concentration values. This level of sikor consumption exceeds the permitted maximum tolerable daily intake (PMTDI) of inorganic As by almost 2-fold. Conclusion: We conclude that sikor can be a significant source of As, Cd and Pb exposure for the Bangladeshi population consuming large quantities of this material. Of particular concern in this regard is geophagy practiced by pregnant women concurrently exposed to As contaminated drinking water. Future studies needs to evaluate the bioavailability of As and other elements from sikor and their impact on human health