Application of bioencapsulated proteinases and peptides for wound gealing

peer reviewe

Biodegradable microparticles loaded with thrombin receptor agonist peptide for gastric ulcer treatment in rats

peer reviewedThe aim of the current paper was to elaborate an immobilization method of thrombin receptor agonist peptide (TRAP-6) in biodegradable biocompatible poly(d,l)-lactide-co-glycolide (PLGA) inicroparticles and to demonstrate the effect of the entrapped peptide for tissue repair, namely,for a gastric ulcer treatment in rats. TRAP-6 was entrapped in polymer using w/o/w double emulsion-evaporation technique. The morphology of empty and TRAP-6 loaded microparticles was evaluated by light and scanning electron microscopy (SEM). In vitro release kinetics profile of TRAP-6 from microparticles was studied by HPLC. To investigate gastric mucosal protection effect in vivo, TRAP-6-loaded microparticles were administered in a rat stomach after a previous mucosal injury (a gastric ulcer). Microparticles with entrapped TRAP-6 were found to reduce both an inflammation and proliferation phases of wound healing, and thus accelerated tissue repair in rats.Programme de travail Région Wallonne/Fédération de Russie 2001- 2002, Art. 3. , Projet de coopération n°3, en collaboration avec : Shemyakin Institute Bioorganic Chemistry, Moscow, Professor Vitali P. Zubov et du Dr. Elena Markvichev

Thrombin receptor agonist peptide immobilized in microspheres stimulates reparative processes in rats with gastric ulcer

The effect of synthetic thrombin receptor (PAR1) agonist peptide encapsulated in microspheres made of lactic and glycolic acid copolymer on tissue reparation was studied in rats with acetate-induced ulcer. PAR1 agonist peptide was immobilized in biodegraded lactic and glycolic acid microspheres by double emulgation, the kinetics of peptide release was analyzed, and the dynamics of ulcer healing was studied in experimental (administration of microspheres with the peptide into the stomach) and two control groups (administration of saline or spheres without peptide). Thrombin receptor agonist peptide gradually released from lactic and glycolic acid microspheres into the stomach shortened the inflammation phase and shifted the proliferation phase to the earlier period, thus accelerating healing of experimental ulcers in rats