9 research outputs found
Characterization of LhSorP5CS, a gene catalyzing proline synthesis in Oriental hybrid lily Sorbonne: molecular modelling and expression analysis
Get PDFCollective cargo hauling by a bundle of parallel microtubules: bi-directional motion caused by load-dependent polymerization and depolymerization
No full textGenetic transformation of a hepatoprotective plant, Phyllanthus amarus
No full textPhyllanthus amarus Schum & Thonn. is a source
of various pharmacologically active compounds such as
phyllanthin, hypophyllanthin, gallic acid, catechin, and
nirurin, a flavone glycoside. A genetic transformation
method using Agrobacterium tumefaciens was developed
for this plant species for the first time. Shoot tips of full
grown plants were used as explants for Agrobacteriummediated
transformation. Transgenic plants were obtained
by co-cultivation of shoot tips explants and A. tumefaciens
strain LBA4404 containing the pCAMBIA 2301 plasmid
harboring neomycin phosphotransferase II (NPT II) and β-
glucuronidase encoding (GUS) genes in the T-DNA region
in the presence of 200 μM acetosyringone. Integration of
the NPT II gene into the genome of transgenic plants was
verified by PCR and Southern blot analyses. Expression of
the NPT II gene was confirmed by RT-PCR analysis. An
average of 25 explants was used, out of which an average
of 19 explants produced kanamycin-resistant shoots, which
rooted to produce 13 complete transgenic plants
High frequency in vitro regeneration system for conservation of Coleus forskohlii: a threatened medicinal herb
No full textExpression of the Vigna aconitifolia P5CSF129A gene in transgenic pigeonpea enhances proline accumulation and salt tolerance
No full textA viable alternative in vitro system and comparative metabolite profiling of different tissues for the conservation of Ceropegia karulensis
No full text
