Concerted action of human chorionic gonadotropin and norepinephrine on intracellular-free calcium in human granulosa-lutein cells

Luteal cells are known to possess receptors for LH/hCG and receptors of the beta-adrenergic type. Interactions of specific agonists with either receptor lead to the activation of adenylate cyclase and subsequently to an increase of cAMP. Since in the human there is also evidence for the presence of alpha-adrenergic receptors, we have investigated whether activation of these receptors is linked to calcium as a second messenger and performed measurement of intracellular free calcium (Ca2+) with Fura-2 in single human granulosa-lutein cells. Addition of either hCG (100, 1,000, 25,000 IU/L) or norepinephrine (NE; known to interact with both alpha- and beta-adrenergic receptors), beta- adrenergic receptor agonist isoproterenol (ISO), or alpha-adrenergic receptor agonist phenylephrine (PHE; all at 10 and 100 mumol/L) did not increase free intracellular Ca2+. However, the addition of combinations of NE/hCG, PHE/hCG, but not the combination ISO/hCG, induced a transient increase in cytosolic free Ca2+. The NE/hCG-evoked calcium signal was not abolished in the presence of the beta-adrenergic receptor antagonist propranolol and was not affected by removal of extracellular Ca2+. Furthermore, we tested whether catecholamines affected the release of progesterone in the presence or absence of hCG. As expected, hCG (10,000 IU/L) stimulated progesterone release by cultured granulosa-lutein cells. When these cells were incubated with NE, PHE, or ISO (at 10 mumol/L), production of progesterone by these cells was not affected. However, the combinations of NE and PHE with hCG abolished the hCG-induced progesterone accumulation, but ISO coincubated with hCG did not. Taken together, our results indicate: 1) the presence of functional alpha-adrenergic receptors on human granulosa-lutein cells; 2) simultaneous activation of two different receptors (for hCG and alpha-agonists) are able to evoke intracellular Ca2+ elevation, implicating postreceptor interactions in human granulosa lutein cells; 3) this process occurs even in the absence of extracellular Ca2+, indicating the involvement of intracellular Ca2+ stores, most likely due to activation of phosphoinositide pathway; 4) catecholamines most likely acting via alpha-adrenergic receptors, inhibit the LH/hCG-induced release of progesterone

AC Stark shift of the Cs microwave atomic clock transitions

We analyze the AC Stark shift of the Cs microwave atomic clock transition theoretically and experimentally. Theoretical and experimental data are in a good agreement with each other. Results indicate the absence of a magic wavelength at which there would be no differential shift of the clock states having zero projections of the total angular momentum

Progress in Atomic Fountains at LNE-SYRTE

We give an overview of the work done with the Laboratoire National de M\'etrologie et d'Essais-Syst\`emes de R\'ef\'erence Temps-Espace (LNE-SYRTE) fountain ensemble during the last five years. After a description of the clock ensemble, comprising three fountains, FO1, FO2, and FOM, and the newest developments, we review recent studies of several systematic frequency shifts. This includes the distributed cavity phase shift, which we evaluate for the FO1 and FOM fountains, applying the techniques of our recent work on FO2. We also report calculations of the microwave lensing frequency shift for the three fountains, review the status of the blackbody radiation shift, and summarize recent experimental work to control microwave leakage and spurious phase perturbations. We give current accuracy budgets. We also describe several applications in time and frequency metrology: fountain comparisons, calibrations of the international atomic time, secondary representation of the SI second based on the 87Rb hyperfine frequency, absolute measurements of optical frequencies, tests of the T2L2 satellite laser link, and review fundamental physics applications of the LNE-SYRTE fountain ensemble. Finally, we give a summary of the tests of the PHARAO cold atom space clock performed using the FOM transportable fountain.Comment: 19 pages, 12 figures, 5 tables, 126 reference

Q-Value and Half-Lives for the Double-Beta-Decay Nuclide 110Pd

The 110Pd double-beta decay Q-value was measured with the Penning-trap mass spectrometer ISOLTRAP to be Q = 2017.85(64) keV. This value shifted by 14 keV compared to the literature value and is 17 times more precise, resulting in new phase-space factors for the two-neutrino and neutrinoless decay modes. In addition a new set of the relevant matrix elements has been calculated. The expected half-life of the two-neutrino mode was reevaluated as 1.5(6) E20 yr. With its high natural abundance, the new results reveal 110Pd to be an excellent candidate for double-beta decay studies

Precision Mass Measurements of 129-131Cd and Their Impact on Stellar Nucleosynthesis via the Rapid Neutron Capture Process

Masses adjacent to the classical waiting-point nuclide 130Cd have been measured by using the Penning- trap spectrometer ISOLTRAP at ISOLDE/CERN. We find a significant deviation of over 400 keV from earlier values evaluated by using nuclear beta-decay data. The new measurements show the reduction of the N = 82 shell gap below the doubly magic 132Sn. The nucleosynthesis associated with the ejected wind from type-II supernovae as well as from compact object binary mergers is studied, by using state-of-the-art hydrodynamic simulations. We find a consistent and direct impact of the newly measured masses on the calculated abundances in the A = 128 - 132 region and a reduction of the uncertainties from the precision mass input data

Probing the N = 32 shell closure below the magic proton number Z = 20: Mass measurements of the exotic isotopes 52,53K

The recently confirmed neutron-shell closure at N = 32 has been investigated for the first time below the magic proton number Z = 20 with mass measurements of the exotic isotopes 52,53K, the latter being the shortest-lived nuclide investigated at the online mass spectrometer ISOLTRAP. The resulting two-neutron separation energies reveal a 3 MeV shell gap at N = 32, slightly lower than for 52Ca, highlighting the doubly-magic nature of this nuclide. Skyrme-Hartree-Fock-Boguliubov and ab initio Gorkov-Green function calculations are challenged by the new measurements but reproduce qualitatively the observed shell effect.Comment: 5 pages, 5 figure

Atomic masses of intermediate-mass neutron-deficient nuclei with relative uncertainty down to 35-ppb via multireflection time-of-flight mass spectrograph

High-precision mass measurements of $^{63}$Cu, $^{64-66}$Zn, $^{65}$Ga, $^{65-67}$Ge, $^{67}$As, $^{78,81}$Br, $^{80}$Rb, and $^{79}$Sr were performed utilizing a multireflection time-of-flight mass spectrograph combined with the gas-filled recoil ion separator GARIS-II. In the case of $^{65}$Ga, a mass uncertainty of 2.1 keV, corresponding to a relative precision of $\delta m / m = 3.5\times10^{-8}$, was obtained and the mass value is in excellent agreement with the 2016 Atomic Mass Evaluation. For $^{67}$Ge and $^{81}$Br, where masses were previously deduced through indirect measurements, discrepancies with literature values were found. The feasibility of using this device for mass measurements of nuclides more neutron-deficient side, which have significant impact on the $rp$-process pathway, is discussed.Comment: 15 pages, 6 figures, 1 tabl

Rapidly Rotating Fermions in an Anisotropic Trap

We consider a cold gas of non-interacting fermions in a two dimensional harmonic trap with two different trapping frequencies $\omega_x \leq \omega_y$, and discuss the effect of rotation on the density profile. Depending on the rotation frequency $\Omega$ and the trap anisotropy $\omega_y/\omega_x$, the density profile assumes two qualitatively different shapes. For small anisotropy ($\omega_y/\omega_x \ll \sqrt{1+4 \Omega^2/\omega_x^2}$), the density consists of elliptical plateaus of constant density, corresponding to Landau levels and is well described by a two dimensional local density approximation. For large anisotropy ($\omega_y/\omega_x \gg \sqrt{1+4 \Omega^2/\omega_x^2}$), the density profile is Gaussian in the strong confining direction and semicircular with prominent Friedel oscillations in the weak direction. In this regime, a one dimensional local density approximation is well suited to describe the system. The crossover between the two regimes is smooth where the step structure between the Landau level edges turn into Friedel oscillations. Increasing the temperature causes the step structure or the Friedel oscillations to wash out leaving a Boltzmann gas density profile.Comment: 14 pages, 7 figure

Splitting of a doubly quantized vortex through intertwining in Bose-Einstein condensates

The stability of doubly quantized vortices in dilute Bose-Einstein condensates of 23Na is examined at zero temperature. The eigenmode spectrum of the Bogoliubov equations for a harmonically trapped cigar-shaped condensate is computed and it is found that the doubly quantized vortex is spectrally unstable towards dissection into two singly quantized vortices. By numerically solving the full three-dimensional time-dependent Gross-Pitaevskii equation, it is found that the two singly quantized vortices intertwine before decaying. This work provides an interpretation of recent experiments [A. E. Leanhardt et al. Phys. Rev. Lett. 89, 190403 (2002)].Comment: 4 pages, 3 figures (to be published in PRA

Vortex oscillations in confined Bose-Einstein condensate interacting with 1D optical lattice

We study Bose-Einstein condensate of atomic Boson gases trapped in a composite potential of a harmonic potential and an optical lattice potential. We found a series of collective excitations that induces localized vortex oscillations with a characteristic wavelength. The oscillations might be observed experimentally when radial confinement is tight. We present the excitation spectra of the vortex oscillation modes and propose a way to experimentally excite the modes.Comment: 5 pages, 7 figures. Title, abstract and references are update