Cross-protection against European swine influenza viruses in the context of infection immunity against the 2009 pandemic H1N1 virus : studies in the pig model of influenza

Pigs are natural hosts for the same influenza virus subtypes as humans and are a valuable model for cross-protection studies with influenza. In this study, we have used the pig model to examine the extent of virological protection between a) the 2009 pandemic H1N1 (pH1N1) virus and three different European H1 swine influenza virus (SIV) lineages, and b) these H1 viruses and a European H3N2 SIV. Pigs were inoculated intranasally with representative strains of each virus lineage with 6- and 17-week intervals between H1 inoculations and between H1 and H3 inoculations, respectively. Virus titers in nasal swabs and/or tissues of the respiratory tract were determined after each inoculation. There was substantial though differing cross-protection between pH1N1 and other H1 viruses, which was directly correlated with the relatedness in the viral hemagglutinin (HA) and neuraminidase (NA) proteins. Cross-protection against H3N2 was almost complete in pigs with immunity against H1N2, but was weak in H1N1/pH1N1-immune pigs. In conclusion, infection with a live, wild type influenza virus may offer substantial cross-lineage protection against viruses of the same HA and/or NA subtype. True heterosubtypic protection, in contrast, appears to be minimal in natural influenza virus hosts. We discuss our findings in the light of the zoonotic and pandemic risks of SIVs

HD 41641: A classical δ\delta Sct-type pulsator with chemical signatures of an Ap star

Among the known groups of pulsating stars, $\delta$ Sct stars are one of the least understood. Theoretical models do not predict the oscillation frequencies that observations reveal. Complete asteroseismic studies are necessary to improve these models and better understand the internal structure of these targets. We study the $\delta$ Sct star HD 41641 with the ultimate goal of understanding its oscillation pattern. The target was simultaneously observed by the CoRoT space telescope and the HARPS high-resolution spectrograph. The photometric data set was analyzed with the software package PERIOD04, while FAMIAS was used to analyze the line profile variations. The method of spectrum synthesis was used for spectroscopically determining the fundamental atmospheric parameters and individual chemical abundances. A total of 90 different frequencies was identified and analyzed. An unambiguous identification of the azimuthal order of the surface geometry could only be provided for the dominant p-mode, which was found to be a nonradial prograde mode with m = +1. Using $T_\mathrm{eff}$ and $\log g$, we estimated the mass, radius, and evolutionary stage of HD 41641. We find HD 41641 to be a moderately rotating, slightly evolved $\delta$ Sct star with subsolar overall atmospheric metal content and unexpected chemical peculiarities. HD 41641 is a pure $\delta$ Sct pulsator with p-mode frequencies in the range from 10 d$^{-1}$ to 20 d$^{-1}$. This pulsating star presents chemical signatures of an Ap star and rotational modulation due to surface inhomogeneities, which we consider indirect evidence of the presence of a magnetic field.Comment: 11 pages, 11 figures, accepted for publication in A&

Gravity-mode period spacings as seismic diagnostic for a sample of gamma Doradus stars from Kepler space photometry and high-resolution ground-based spectroscopy

Gamma Doradus stars (hereafter gamma Dor stars) are gravity-mode pulsators of spectral type A or F. Such modes probe the deep stellar interior, offering a detailed fingerprint of their structure. Four-year high-precision space-based Kepler photometry of gamma Dor stars has become available, allowing us to study these stars with unprecedented detail. We selected, analysed, and characterized a sample of 67 gamma Dor stars for which we have Kepler observations available. For all the targets in the sample we assembled high-resolution spectroscopy to confirm their F-type nature. We found fourteen binaries, among which four single-lined binaries, five double-lined binaries, two triple systems and three binaries with no detected radial velocity variations. We estimated the orbital parameters whenever possible. For the single stars and the single-lined binaries, fundamental parameter values were determined from spectroscopy. We searched for period spacing patterns in the photometric data and identified this diagnostic for 50 of the stars in the sample, 46 of which are single stars or single-lined binaries. We found a strong correlation between the spectroscopic vsini and the period spacing values, confirming the influence of rotation on gamma Dor-type pulsations as predicted by theory. We also found relations between the dominant g-mode frequency, the longest pulsation period detected in series of prograde modes, vsini, and log Teff.Comment: 61 pages, 61 figures, 6 tables, accepted for publication in ApJ

Infection of swine ex vivo tissues with avian viruses including H7N9 and correlation with glycomic analysis

OBJECTIVES: Swine have been regarded as intermediate hosts in the spread of influenza from birds to humans but studies of the sialylated glycans that comprise their respiratory tract have not been extensively studied in the past. This study analyzed the sialylated N-glycan and O-glycan profile of swine trachea and lung and correlated this with ex-vivo infection of swine explants with avian influenza viruses. SAMPLE: Lungs and tracheal samples were obtained from normal farm and laboratory raised swine and used for ex vivo infection as well as mass spectrometric analysis. Infection of the ex vivo tissues used high pathogenic and low pathogenic avian viruses including the novel H7N9 virus that emerged in China in early 2013. MAIN OUTCOME MEASURES: Assessment of successful replication was determined by TCID50 as well as virus immunohistochemistry. The N-glycan and O-glycan profiles were measured by MALDI-TOF and sialylated linkages were determined by sialidase treatment. Lectin binding histochemistry was also performed on formalin fixed tissue samples with positive binding detected by chromogen staining. RESULTS: The swine respiratory tract glycans differed from the human respiratory tact glycans in two main areas. There was a greater abundance of Gal-α-Gal linkages resulting in a relative decrease in sialylated glycans. The swine respiratory tract also had a greater proportion of glycans containing Neu5Gc and Siaα2-6 glycans than the human respiratory tract. Infection with avian viruses was confined primarily to lung bronchioles rather than trachea and parenchyma. CONCLUSIONS: In contrast to previous studies we found that there was not as much expression of Siaα2-3 glycans on the surface of the trachea. Infection of Siaα2-3 binding avian viruses was restricted to the lower respiratory tract bronchioles. This finding may diminish the ability of the swine to act as an intermediary in the transmission of avian viruses to humans.published_or_final_versio

Detecting non-uniform period spacings in the Kepler photometry of gamma Doradus stars: methodology and case studies

Context. The analysis of stellar oscillations is one of the most reliable ways to probe stellar interiors. Recent space missions such as Kepler have provided us with an opportunity to study these oscillations with unprecedented detail. For many multi-periodic pulsators such as {\gamma} Doradus stars, this led to the detection of dozens to hundreds of oscillation frequencies that could not be found from ground-based observations. Aims. We aim to detect non-uniform period spacings in the Fourier spectra of a sample of {\gamma} Doradus stars observed by Kepler. Such detection is complicated by both the large number of significant frequencies in the space photometry and by overlapping non-equidistant rotationally split multiplets. Methods. Guided by theoretical properties of gravity-mode oscillation of {\gamma} Doradus stars, we developed a period-spacing detection method and applied it to Kepler observations of a few stars, after having tested the performance from simulations. Results. The application of the technique resulted in the clear detection of non-uniform period spacing series for three out of the five treated Kepler targets. Disadvantages of the technique are also discussed, and include the disability to distinguish between different values of the spherical degree and azimuthal order of the oscillation modes without additional theoretical modelling. Conclusions. Despite the shortcomings, the method is shown to allow solid detections of period spacings for {\gamma} Doradus stars, which will allow future asteroseismic analyses of these stars.Comment: 10 pages, 13 figures, 2 tables. Accepted for publication in Astronomy & Astrophysic