RhoA function in lamellae formation and migration is regulated by the alpha6beta4 integrin and cAMP metabolism

Clone A colon carcinoma cells develop fan-shaped lamellae and exhibit random migration when plated on laminin, processes that depend on the ligation of the alpha6beta4 integrin. Here, we report that expression of a dominant negative RhoA (N19RhoA) in clone A cells inhibited alpha6beta4-dependent membrane ruffling, lamellae formation, and migration. In contrast, expression of a dominant negative Rac (N17Rac1) had no effect on these processes. Using the Rhotekin binding assay to assess RhoA activation, we observed that engagement of alpha6beta4 by either antibody-mediated clustering or laminin attachment resulted in a two- to threefold increase in RhoA activation, compared with cells maintained in suspension or plated on collagen. Antibody-mediated clustering of beta1 integrins, however, actually suppressed Rho A activation. The alpha6beta4-mediated interaction of clone A cells with laminin promoted the translocation of RhoA from the cytosol to membrane ruffles at the edges of lamellae and promoted its colocalization with beta1 integrins, as assessed by immunofluorescence microscopy. In addition, RhoA translocation was blocked by inhibiting phosphodiesterase activity and enhanced by inhibiting the activity of cAMP-dependent protein kinase. Together, these results establish a specific integrin-mediated pathway of RhoA activation that is regulated by cAMP and that functions in lamellae formation and migration

Shapley Supercluster Survey: Construction of the photometric catalogues and i-band data release

The Shapley Supercluster Survey is a multi-wavelength survey covering an area of ∼23 deg² (∼260 Mpc² at z = 0.048) around the supercluster core, including nine Abell and two poor clusters, having redshifts in the range 0.045–0.050. The survey aims to investigate the role of the cluster-scale mass assembly on the evolution of galaxies, mapping the effects of the environment from the cores of the clusters to their outskirts and along the filaments. The optical (ugri) imaging acquired with OmegaCAM on the VLT Survey Telescope is essential to achieve the project goals providing accurate multi-band photometry for the galaxy population down to m∗ + 6. We describe the methodology adopted to construct the optical catalogues and to separate extended and point-like sources. The catalogues reach average 5σ limiting magnitudes within a 3 arcsec diameter aperture of ugri = [24.4,24.6,24.1,23.3] and are 93 per cent complete down to ugri = [23.8,23.8,23.5,22.0] mag, corresponding to ∼m∗ r + 8.5. The data are highly uniform in terms of observing conditions and all acquired with seeing less than 1.1 arcsec full width at half-maximum. The median seeing in r band is 0.6 arcsec, corresponding to 0.56 kpc h⁻¹ 70 at z = 0.048. While the observations in the u, g and r bands are still ongoing, the i-band observations have been completed, and we present the i-band catalogue over the whole survey area. The latter is released and it will be regularly updated, through the use of the Virtual Observatory tools. This includes 734 319 sources down to i = 22.0 mag and it is the first optical homogeneous catalogue at such a depth, covering the central region of the Shapley supercluster

Discovery of a faint, star-forming, multiply lensed, Lyman-alpha blob

We report the discovery of a multiply lensed Lyman-$\alpha$ blob (LAB) behind the galaxy cluster AS1063 using the Multi Unit Spectroscopic Explorer (MUSE) on the Very Large Telescope (VLT). The background source is at $z=$ 3.117 and is intrinsically faint compared to almost all previously reported LABs. We used our highly precise strong lensing model to reconstruct the source properties, and we find an intrinsic luminosity of $L_{\rm Ly\alpha}$=$1.9\times10^{42}$ erg s$^{-1}$, extending to 33 kpc. We find that the LAB is associated with a group of galaxies, and possibly a protocluster, in agreement with previous studies that find LABs in overdensities. In addition to Lyman-$\alpha$ (Ly$\alpha$) emission, we find \ion{C}{IV}, \ion{He}{II}, and \ion{O}{III}] ultraviolet (UV) emission lines arising from the centre of the nebula. We used the compactness of these lines in combination with the line ratios to conclude that the \Lya nebula is likely powered by embedded star formation. Resonant scattering of the \Lya photons then produces the extended shape of the emission. Thanks to the combined power of MUSE and strong gravitational lensing, we are now able to probe the circumgalatic medium of sub-$L_{*}$ galaxies at $z\approx 3$.Comment: 7 pages, 7 figures; moderate changes to match the accepted A&A versoi

Ionising the Intergalactic Medium by Star Clusters: The first empirical evidence

We present a VLT/X-Shooter spectroscopy of the Lyman continuum (LyC) emitting galaxy 'Ion2' at z=3.2121 and compare it to that of the recently discovered strongly lensed LyC-emitter at z=2.37, known as the 'Sunburst' arc. Three main results emerge from the X-Shooter spectrum: (a) the Lya has three distinct peaks with the central one at the systemic redshift, indicating a ionised tunnel through which both Lya and LyC radiation escape; (b) the large O32 oxygen index ([OIII]4959-5007 / [OII]3727-3729) of 9.18(-1.32/+1.82) is compatible to those measured in local (z~0.4) LyC leakers; (c) there are narrow nebular high-ionisation metal lines with \sigma_v < 20 km/s, which confirms the presence of young hot, massive stars. The HeII1640 appears broad, consistent with a young stellar component including Wolf-Rayet stars. Similarly, the Sunburst LyC-emitter shows a triple-peaked Lya profile and from VLT/MUSE spectroscopy the presence of spectral features arising from young hot and massive stars. The strong lensing magnification, (\mu > 20), suggests that this exceptional object is a gravitationally-bound star cluster observed at a cosmological distance, with a stellar mass M <~ 10^7 Msun and an effective radius smaller than 20 pc. Intriguingly, sources like Sunburst but without lensing magnification might appear as Ion2-like galaxies, in which unresolved massive star clusters dominate the ultraviolet emission. This work supports the idea that dense young star clusters can contribute to the ionisation of the IGM through holes created by stellar feedback.Comment: 13 pages, 9 figures and 1 table, MNRAS accepted. Some typos fixe

CLASH-VLT: Testing the Nature of Gravity with Galaxy Cluster Mass Profiles

We use high-precision kinematic and lensing measurements of the total mass profile of the dynamically relaxed galaxy cluster MACS J1206.2-0847 at $z=0.44$ to estimate the value of the ratio $\eta=\Psi/\Phi$ between the two scalar potentials in the linear perturbed Friedmann-Lemaitre-Robertson-Walker metric.[...] Complementary kinematic and lensing mass profiles were derived from exhaustive analyses using the data from the Cluster Lensing And Supernova survey with Hubble (CLASH) and the spectroscopic follow-up with the Very Large Telescope (CLASH-VLT). Whereas the kinematic mass profile tracks only the time-time part of the perturbed metric (i.e. only $\Phi$), the lensing mass profile reflects the contribution of both time-time and space-space components (i.e. the sum $\Phi+\Psi$). We thus express $\eta$ as a function of the mass profiles and perform our analysis over the radial range $0.5\,Mpc\le r\le r_{200}=1.96\,Mpc$. Using a spherical Navarro-Frenk-White mass profile, which well fits the data, we obtain \eta(r_{200})=1.01\,_{-0.28}^{+0.31} at the 68\% C.L. We discuss the effect of assuming different functional forms for mass profiles and of the orbit anisotropy in the kinematic reconstruction. Interpreting this result within the well-studied $f(R)$ modified gravity model, the constraint on $\eta$ translates into an upper bound to the interaction length (inverse of the scalaron mass) smaller than 2 Mpc. This tight constraint on the $f(R)$ interaction range is however substantially relaxed when systematic uncertainties in the analysis are considered. Our analysis highlights the potential of this method to detect deviations from general relativity, while calling for the need of further high-quality data on the total mass distribution of clusters and improved control on systematic effects.Comment: 18 pages, 3 figures, submitted to JCA

Three-body decay of 6^{6}Be

Three-body correlations for the ground-state decay of the lightest two-proton emitter $^{6}$Be are studied both theoretically and experimentally. Theoretical studies are performed in a three-body hyperspherical-harmonics cluster model. In the experimental studies, the ground state of $^{6}$Be was formed following the $\alpha$ decay of a $^{10}$C beam inelastically excited through interactions with Be and C targets. Excellent agreement between theory and experiment is obtained demonstrating the existence of complicated correlation patterns which can elucidate the structure of $^{6}$Be and, possibly, of the A=6 isobar.Comment: 17 pages, 21 figures, 5 table

Seeded x-ray free-electron laser generating radiation with laser statistical properties

The invention of optical lasers led to a revolution in the field of optics and even to the creation of completely new fields of research such as quantum optics. The reason was their unique statistical and coherence properties. The newly emerging, short-wavelength free-electron lasers (FELs) are sources of very bright coherent extreme-ultraviolet (XUV) and x-ray radiation with pulse durations on the order of femtoseconds, and are presently considered to be laser sources at these energies. Most existing FELs are highly spatially coherent but in spite of their name, they behave statistically as chaotic sources. Here, we demonstrate experimentally, by combining Hanbury Brown and Twiss (HBT) interferometry with spectral measurements that the seeded XUV FERMI FEL-2 source does indeed behave statistically as a laser. The first steps have been taken towards exploiting the first-order coherence of FELs, and the present work opens the way to quantum optics experiments that strongly rely on high-order statistical properties of the radiation.Comment: 24 pages, 10 figures, 37 reference

Shapley Supercluster Survey (ShaSS): Galaxy Evolution from Filaments to Cluster Cores

We present an overview of a multi-wavelength survey of the Shapley supercluster (SSC; z~0.05) covering a contiguous area of 260 h^-2_70 Mpc^2 including the supercluster core. The project main aim is to quantify the influence of cluster-scale mass assembly on galaxy evolution in one of the most massive structures in the local Universe. The Shapley supercluster survey (ShaSS) includes nine Abell clusters (A3552, A3554, A3556, A3558, A3559, A3560, A3562, AS0724, AS0726) and two poor clusters (SC1327- 312, SC1329-313) showing evidence of cluster-cluster interactions. Optical (ugri) and near-infrared (K) imaging acquired with VST and VISTA allow us to study the galaxy population down to m*+6 at the supercluster redshift. A dedicated spectroscopic survey with AAOmega on the Anglo-Australian Telescope provides a magnitude-limited sample of supercluster members with 80% completeness at ~m*+3. We derive the galaxy density across the whole area, demonstrating that all structures within this area are embedded in a single network of clusters, groups and filaments. The stellar mass density in the core of the SSC is always higher than 9E09 M_sun Mpc^-3, which is ~40x the cosmic stellar mass density for galaxies in the local Universe. We find a new filamentary structure (~7 Mpc long in projection) connecting the SSC core to the cluster A3559, as well as previously unidentified density peaks. We perform a weak-lensing analysis of the central 1 sqdeg field of the survey obtaining for the central cluster A3558 a mass of M_500=7.63E14 M_sun, in agreement with X-ray based estimates.Comment: 22 pages, 11 figures. Accepted for publication on MNRA

Direct Lyman continuum and Lyman-alpha escape observed at redshift 4

We report on the serendipitous discovery of a z=4.0, M1500=-22.20 star-forming galaxy (Ion3) showing copious Lyman continuum (LyC) leakage (~60% escaping), a remarkable multiple peaked Lya emission, and significant Lya radiation directly emerging at the resonance frequency. This is the highest redshift confirmed LyC emitter in which the ionising and Lya radiation possibly share a common ionised cavity (with N_HI<10^17.2 cm^-2). Ion3 is spatially resolved, it shows clear stellar winds signatures like the P-Cygni NV1240 profile, and has blue ultraviolet continuum (\beta = -2.5 +/- 0.25, F_\lambda~ \lambda^\beta) with weak low-ionisation interstellar metal lines. Deep VLT/HAWKI Ks and Spitzer/IRAC 3.6um and 4.5um imaging show a clear photometric signature of the Halpha line with equivalent width of 1000A rest-frame emerging over a flat continuum (Ks-4.5um ~ 0). From the SED fitting we derive a stellar mass of 1.5x10^9 Msun, SFR of 140 Msun/yr and age of ~10 Myr, with a low dust extinction, E(B-V)< 0.1, placing the source in the starburst region of the SFR-M^* plane. Ion3 shows similar properties of another LyC emitter previously discovered (z=3.21, Ion2, Vanzella et al. 2016). Ion3 (and Ion2) represents ideal high-redshift reference cases to guide the search for reionising sources at z>6.5 with JWST.Comment: Accepted for publication in MNRAS. 5 pages, 4 figures, 1 tabl

The story of supernova 'Refsdal' told by MUSE

We present MUSE observations in the core of the HFF galaxy cluster MACS J1149.5+2223, where the first magnified and spatially-resolved multiple images of SN 'Refsdal' at redshift 1.489 were detected. Thanks to a DDT program with the VLT and the extraordinary efficiency of MUSE, we measure 117 secure redshifts with just 4.8 hours of total integration time on a single target pointing. We spectroscopically confirm 68 galaxy cluster members, with redshift values ranging from 0.5272 to 0.5660, and 18 multiple images belonging to 7 background, lensed sources distributed in redshifts between 1.240 and 3.703. Starting from the combination of our catalog with those obtained from extensive spectroscopic and photometric campaigns using the HST, we select a sample of 300 (164 spectroscopic and 136 photometric) cluster members, within approximately 500 kpc from the BCG, and a set of 88 reliable multiple images associated to 10 different background source galaxies and 18 distinct knots in the spiral galaxy hosting SN 'Refsdal'. We exploit this valuable information to build 6 detailed strong lensing models, the best of which reproduces the observed positions of the multiple images with a rms offset of only 0.26". We use these models to quantify the statistical and systematic errors on the predicted values of magnification and time delay of the next emerging image of SN 'Refsdal'. We find that its peak luminosity should should occur between March and June 2016, and should be approximately 20% fainter than the dimmest (S4) of the previously detected images but above the detection limit of the planned HST/WFC3 follow-up. We present our two-dimensional reconstruction of the cluster mass density distribution and of the SN 'Refsdal' host galaxy surface brightness distribution. We outline the roadmap towards even better strong lensing models with a synergetic MUSE and HST effort.Comment: 21 pages, 9 figures, 6 tables; accepted for publication in the Astrophysical Journal - extra information on data analysis added, all model predictions and results unchange