Doubling grain Fe and Zn concentration in sorghum to combat the micronutrient malnutrition in sorghum eating populations

Dietary induced micronutrient malnutrition (MNM) is one of the greatest global challenges of our times and India has largest number of malnourished people globally. Sorghum is among the major staples and a cheapest sources of micronutrients therefore, biofortification of sorghum is of high priority. From screening of more than 4000 accessions and breeding lines we identified promising donors for Fe and Zn and established the genetic control. Fe and Zn are quantitatively inherited. While grain Zn in predominantly under additive gene control, non-additive gene actions also has role in controlling grain Fe. To develop hybrids with high Fe and Zn both parents should have high Fe and Zn. We demonstrated the prediction of F1 hybrid performance based on mid-parental value for Fe and Zn. Both Fe and Zn are positively correlated (r=0.6 to 0.8) and simultaneous improvement for Fe and Zn is feasible. Using RIL population sorghum genetic map was constructed with 2,088 markers (1148 DArTs, 927 DArTSeqs and 13 SSRs) covering 1355.52 cM with an average marker interval of 0.6cM. Forty-seven QTLs (individual) and 7 QTLs (across) environments with small maineffect and 21 co-localized QTLs for Fe and Zn were identified

Genetic Enhancement Perspectives and Prospects for Grain Nutrients Density

Diet-induced micronutrient malnutrition continues to be a major challenge globally, especially in the developing world. With the ever-increasing population, it becomes a daunting task to feed millions of mouths with nutritious food. It is time to reorient agricultural systems to produce quality food to supply the calorie and nutrient requirements needed by the human body. Biofortification is the process of improving micronutrients density by genetic means. It is cheaper and sustainable and complements well with the nutrient supplementation and fortification— the short-term strategies that are currently deployed to address the micronutrient malnutrition. Sorghum is one of the important food crops globally, adapted to semi-arid tropics, and there is increased awareness on its nutritional importance. Further, there is great opportunity to improve sorghum for nutritional quality. This chapter deals about the genetic enhancement perspectives and prospects for improving the nutritional quality with main emphasis on grain micronutrient density in sorghum

Genetic and genomic resources, and breeding for accelerating improvement of small millets: current status and future interventions

Current agricultural and food systems encourage research and development on major crops, neglecting regionally important minor crops. Small millets include a group of small- seeded cereal crops of the grass family Poaceae. This includes finger millet, foxtail millet, proso millet, barnyard millet, kodo millet, little millet, teff, fonio, job’s tears, guinea millet, and browntop millet. Small millets are an excellent choice to supplement major staple foods for crop and dietary diversity because of their diverse adaptation on marginal lands, less water requirement, lesser susceptibility to stresses, and nutritional superiority compared to major cereal staples. Growing interest among consumers about healthy diets together with climate-resilient features of small millets underline the necessity of directing more research and development towards these crops. Except for finger millet and foxtail millet, and to some extent proso millet and teff, other small millets have received minimal research attention in terms of development of genetic and genomic resources and breeding for yield enhancement. Considerable breeding efforts were made in finger millet and foxtail millet in India and China, respectively, proso millet in the United States of America, and teff in Ethiopia. So far, five genomes, namely foxtail millet, finger millet, proso millet, teff, and Japanese barnyard millet, have been sequenced, and genome of foxtail millet is the smallest (423-510 Mb) while the largest one is finger millet (1.5 Gb). Recent advances in phenotyping and genomics technologies, together with available germplasm diversity, could be utilized in small millets improvement. This review provides a comprehensive insight into the importance of small millets, the global status of their germplasm, diversity, promising germplasm resources, and breeding approaches (conventional and genomic approaches) to accelerate climate-resilient and nutrient-dense small millets for sustainable agriculture, environment, and healthy food systems

Identification of QTLs and candidate genes for high grain Fe and Zn concentration in sorghum [Sorghum bicolor (L.)Moench]

Sorghum is a major food crop in the semi-arid tropics of Africa and Asia. Enhancing the grain iron (Fe) and zinc (Zn) concentration in sorghum using genetic approaches would help alleviate micronutrient malnutrition in millions of poor people consuming sorghum as a staple food. To localize genomic regions associated with grain Fe and Zn, a sorghum F6 recombinant inbred line (RIL) population (342 lines derived from cross 296B PVK 801) was phenotyped in six environments, and genotyped with simple sequence repeat (SSR), DArT (Diversity Array Technology) and DArTSeq (Diversity Array Technology) markers. Highly significant genotype environment interactions were observed for both micronutrients. Grain Fe showed greater variation than Zn. A sorghum genetic map was constructed with 2088 markers (1148 DArTs, 927 DArTSeqs and 13 SSRs) covering 1355.52 cM with an average marker interval of 0.6 cM. Eleven QTLs (individual) and 3 QTLs (across) environments for Fe and Zn were identified. We identified putative candidate genes from the QTL interval of qfe7.1, qzn7.1, and qzn7.2 (across environments) located on SBI-07 involved in Fe and Zn metabolism. These were CYP71B34, and ZFP 8 (ZINC FINGER PROTEIN 8). After validation, the linked markers identified in this study can help in developing high grain Fe and Zn sorghum cultivars in sorghum improvement programs globally

Identification of QTLs and Underlying Candidate Genes Controlling Grain Fe and Zn Concentration in Sorghum [Sorghum bicolor (L).Moench]

Biofortification is one of sustainable options for combating micronutrient-malnutrition. For identifying genomic regions associated with grain Fe and Zn in sorghum, RIL population (342 individuals) from cross 296B × PVK 801 was phenotyped for two years at three locations and genotyped with SSRs and DArTs. Highly significant genotype×environment interactions were observed for both micronutrients; grain Fe showed greater variation than Zn. Sorghum genetic map was constructed with 2088 markers (1148 DArTs, 927 DArT Seqs and 13 SSRs) covering 1355.52 cM with an average marker interval of 0.6cM. A total of 18 QTLs controlling Fe and Zn were found stable across environments. Three QTLs for Fe and 15 for Zn were identified with phenotypic variance explained (PVE) values ranging from 3.94 to 5.09% and 3.17 to 9.42%, respectively. Of these 18 stable QTLs, 11 were located on chromosome SBI-07. Favorable alleles for 11 QTLs (co-located) for Fe and Zn on chromosome SBI-07 were contributed by parent PVK801-P23. QTLs were analyzed in-silico to identify underlying candidate genes, 62 candidate genes involved in Fe/Zn metabolism were identified within QTL interval; twenty-three were found in QTL with highest phenotypic effect (PVE 9.42%). Sorghum genes underlying Fe/Zn QTLs were used to analyze gene synteny with rice and maize. Synteny sequence level between sorghum-rice ranged from 44% to 97%, while sorghum-maize ranged from 49% to 99%. QTLs/candidate/novel genes along with the marker/genetic resources identified through this study can help in developing high Fe and Zn lines in cost-effective and efficient manner

Genetic Variability, Genotype × Environment Interaction, Correlation, and GGE Biplot Analysis for Grain Iron and Zinc Concentration and Other Agronomic Traits in RIL Population of Sorghum (Sorghum bicolor L. Moench)

The low grain iron and zinc densities are well documented problems in food crops, affecting crop nutritional quality especially in cereals. Sorghum is a major source of energy and micronutrients for majority of population in Africa and central India. Understanding genetic variation, genotype × environment interaction and association between these traits is critical for development of improved cultivars with high iron and zinc. A total of 336 sorghum RILs (Recombinant Inbred Lines) were evaluated for grain iron and zinc concentration along with other agronomic traits for 2 years at three locations. The results showed that large variability exists in RIL population for both micronutrients (Iron = 10.8 to 76.4 mg kg−1 and Zinc = 10.2 to 58.7 mg kg−1, across environments) and agronomic traits. Genotype × environment interaction for both micronutrients (iron and zinc) was highly significant. GGE biplots comparison for grain iron and zinc showed greater variation across environments. The results also showed that G × E was substantial for grain iron and zinc, hence wider testing needed for taking care of G × E interaction to breed micronutrient rich sorghum lines. Iron and zinc concentration showed high significant positive correlation (across environment = 0.79; p 0.60, in individual environments) for Fe and Zn and other traits studied indicating its suitability to map QTL for iron and zinc

Variability and trait‐specific accessions for grain yield and nutritional traits in germplasm of little millet ( Panicum sumatrense Roth. Ex. Roem. & Schult.)

Little millet (Panicum sumatrense Roth. Ex. Roem. & Schult.), a member of the grass family Poaceae, is native to India. It is nutritionally superior to major cereals, grows well on marginal lands, and can withstand drought and waterlogging conditions. Two-hundred diverse little millet landraces were characterized to assess variability for agronomic and nutritional traits and identify promising accessions. Highly significant variabilitywas found for all the agronomic and grain nutrient traits. Accessions of robusta were high yielding whereas those of nana were rich in grain nutrients. About 80% of the accessions showed consistent protein and zinc (Zn) contents whereas iron (Fe) and calcium (Ca) contents were less consistent (29.5 and 63.5%, respectively) over 2 yr. Promising trait-specific accessions were identified for greater seed weight (10 accessions), high grain yield (15), high biomass yield (15), and consistently high grain nutrients (30) over 2 yr (R2 = .69–.74, P ≤ .0001). A few accessions showed consistently high for two or more nutrients (IPmr 449 for Fe, Zn, Ca, and protein; IPmr 981 for Zn and protein). Five accessions (IPmr 855, 974, 877, 897, 767) were high yielding and also rich in Ca. Consumption of 100 g of little millet grains can potentially contribute to the recommended dietary allowance of up to 28% Fe, 37% Zn, and 27% protein. Multilocation evaluation of the promising accessions across different soil types, fertility levels, and climatic conditions would help to identify valuable accessions for direct release as a cultivar or use in little millet improvement

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Not AvailableFoxtail millet, nutritional importance, genetics, breeding methods, varieties released in India and future prospects are covered.Not Availabl

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Not AvailableKodo millet, nutritional importance, breeding methods, varieties released in India, kodo poisoning and future prospects are covered.Not Availabl

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Not AvailableBreeding of short duration groundnut (Arachis hypogaea L .) varieties with enhanced per day productivity is a priority area in the semi-arid tropical regions to evade the end-season moisture deficit stress and for summer cultivation where the harvesting of groundnut coincides with the on-set of monsoon. Eighteen advanced breeding lines were evaluated during rainy and summer seasons for productive capacity and earliness in maturity by estimating the relative reduction in yield and associated traits due to advanced harvesting by 10 or 20 days over normal harvest. During rainy season the test genotypes were also compared with two popular varieties JL 24 and HNG 10 as checks. Harvesting the genotypes 20 days ahead of normal harvest lead to considerable loss in pod and kernel yield, the reduction being larger in summer. When harvesting was advanced by l0 days the relative yield reduction was considerably lower in some of the genotypes (1-4% pod and 3-4% kernel yield reduction during rainy season) and six genotypes recorded superior yield coupled with earliness in comparison to the best check. The genotypes PBS 11029, PBS 21031, PBS 30076, PBS 11066, PBS 15004 and PBS 28008 were found early in maturity during rainy season and PBS 21031 during summer as they registered negligible loss in yield when harvesting was advanced. The proposed concept of least reduction in yield and its component traits when harvested early coupled with high productivity may be used as a field technique for screening large number of advanced breeding lines to identify early and high productive genotypes.Not Availabl