Cointegration analysis with mixed-frequency data

We develop a method for directly modeling cointegrated multivariate time series that are observed in mixed frequencies. We regard lower-frequency data as regularly (or irregularly) missing and treat them with higher-frequency data by adopting a state-space model. This utilizes the structure of multivariate data as well as the available sample information more fully than the methods of transformation to a single frequency, and enables us to estimate parameters including cointegrating vectors and the missing observations of low-frequency data and to construct forecasts for future values. For the maximum likelihood estimation of the parameters in the model, we use an expectation maximization algorithm based on the state-space representation of the error correction model. The statistical efficiency of the developed method is investigated through a Monte Carlo study. We apply the method to a mixed-frequency data set that consists of the quarterly real gross domestic product and the monthly consumer price index

The EICP27 protein of equine herpesvirus 1 is recruited to viral promoters by its interaction with the immediate-early protein

AbstractThe equine herpesvirus 1 (EHV-1) EICP27 protein cooperates with either the immediate-early (IE) or the EICP0 protein to synergistically trans-activate viral promoters. GST-pulldown and co-immunoprecipitation assays revealed that the EICP27 protein's cooperation with the IE or the EICP0 protein involves its physical interaction with these viral proteins. In the case of the IE–EICP27 protein interaction, IE residues 424 to 826 and EICP27 residues 41 to 206 harbor the interactive domains. Electrophoretic mobility shift assays (EMSA) suggested that the EICP27 protein is not a sequence-specific DNA-binding protein as it fails to directly bind to the IE promoter, the early EICP27, EICP0, and TK promoters, or the late gD and IR5 promoters. However, EMSA studies also showed that the interaction of the IE and EICP27 proteins results in the recruitment of the EICP27 protein to representative early promoters. These results support our hypothesis that the EICP27 protein participates in the trans-activation of EHV-1 promoters, and suggest its presence within RNA polymerase II preinitiation complexes that assemble at viral promoters

Iterative in Situ Click Chemistry Assembles a Branched Capture Agent and Allosteric Inhibitor for Akt1

We describe the use of iterative in situ click chemistry to design an Akt-specific branched peptide triligand that is a drop-in replacement for monoclonal antibodies in multiple biochemical assays. Each peptide module in the branched structure makes unique contributions to affinity and/or specificity resulting in a 200 nM affinity ligand that efficiently immunoprecipitates Akt from cancer cell lysates and labels Akt in fixed cells. Our use of a small molecule to preinhibit Akt prior to screening resulted in low micromolar inhibitory potency and an allosteric mode of inhibition, which is evidenced through a series of competitive enzyme kinetic assays. To demonstrate the efficiency and selectivity of the protein-templated in situ click reaction, we developed a novel QPCR-based methodology that enabled a quantitative assessment of its yield. These results point to the potential for iterative in situ click chemistry to generate potent, synthetically accessible antibody replacements with novel inhibitory properties

Shigella flexneri serotype 1c derived from serotype 1a by acquisition of gtrIC gene cluster via a bacteriophage

BACKGROUND: Shigella spp. are the primary causative agents of bacillary dysentery. Since its emergence in the late 1980s, the S. flexneri serotype 1c remains poorly understood, particularly with regard to its origin and genetic evolution. This article provides a molecular insight into this novel serotype and the gtrIC gene cluster that determines its unique immune recognition. RESULTS: A PCR of the gtrIC cluster showed that serotype 1c isolates from different geographical origins were genetically conserved. An analysis of sequences flanking the gtrIC cluster revealed remnants of a prophage genome, in particular integrase and tRNAPro genes. Meanwhile, Southern blot analyses on serotype 1c, 1a and 1b strains indicated that all the tested serotype 1c strains may have had a common origin that has since remained distinct from the closely related 1a and 1b serotypes. The identification of prophage genes upstream of the gtrIC cluster is consistent with the notion of bacteriophage-mediated integration of the gtrIC cluster into a pre-existing serotype. CONCLUSIONS: This is the first study to show that serotype 1c isolates from different geographical origins share an identical pattern of genetic arrangement, suggesting that serotype 1c strains may have originated from a single parental strain. Analysis of the sequence around the gtrIC cluster revealed a new site for the integration of the serotype converting phages of S. flexneri. Understanding the origin of new pathogenic serotypes and the molecular basis of serotype conversion in S. flexneri would provide information for developing cross-reactive Shigella vaccines

The Dynamical Behaviors in (2+1)-Dimensional Gross-Neveu Model with a Thirring Interaction

We analyze (2+1)-dimensional Gross-Neveu model with a Thirring interaction, where a vector-vector type four-fermi interaction is on equal terms with a scalar-scalar type one. The Dyson-Schwinger equation for fermion self-energy function is constructed up to next-to-leading order in 1/N expansion. We determine the critical surface which is the boundary between a broken phase and an unbroken one in ($\alpha_c,~ \beta_c,~ N_c$) space. It is observed that the critical behavior is mainly controlled by Gross-Neveu coupling $\alpha_c$ and the region of the broken phase is separated into two parts by the line $\alpha_c=\alpha_c^*(=\frac{8}{\pi^2})$. The mass function is strongly dependent upon the flavor number N for $\alpha > \alpha_c^*$, while weakly for $\alpha \alpha_c^*$, the critical flavor number $N_c$ increases as Thirring coupling $\beta$ decreases. By driving the CJT effective potential, we show that the broken phase is energetically preferred to the symmetric one. We discuss the gauge dependence of the mass function and the ultra-violet property of the composite operators.Comment: 19 pages, LaTex, 6 ps figure files(uuencoded in seperate file

Histochemical demonstrations of actinomycininduced changes of certain oxidative and hydrolytic enzymes of rat incisor pulps

Levels of succinic and lactic dehydrogenases, cytochrome oxidase, alkaline and acid phosphatase activities of the rat incisor pulp were examined following a sublethal dose injection of actinomycin D. Control animals injected with physiological saline were pair-fed.Succinic dehydrogenase and cytochrome oxidase activities were low in the pulp and presented insignificant alterations throughout the experiment. Lactic dehydrogenase and alkaline phosphatase activities of the dental pulp dropped rapidly during the first several days of the experiment and returned to the normal level by the end of the third week. Acid phosphatase activity showed rapid recovery after an initial drop, reached a peak on day 7, and returned to normal by the end of the second week.The significant increase in acid phosphatase activity occurred at the time when cytoplasmic degenerations are most pronounced. While succinic dehydrogenase and cytochrome oxidase activities remained constant during theexperiment, the normally higher lactic dehydrogenase activity showed a marked reduction in the experimental animals suggesting the dependence of pulp cells on anaerobic glycolysis, which is suppressed by actinomycin D. Variations in the level of enzyme activities observed in control animals are attributed to pair-feeding. Results of the present work give further supports to data obtained from previous ultrastructural studies.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/33370/1/0000768.pd