DETERMINATION OF FUNDAMENTAL PHYSICAL PARAMETERS OF BLOOD SERUM PROTEINS FOR DEVELOPMENT THE METHODS FOR CANCER DIAGNOSIS

Background: A fundamental problem of medical diagnostics as an assessment of human plasma and serum proteins in various pathologic states. Physical molecular and dynamic characteristics of proteins that are significantly changed during a  disease have been proposed as diagnostic parameters. Aim: To conduct fundamental research on molecular properties of serum proteins, such as their mobility, intermolecular interactions, aggregation/formation of nanoscale protein clusters, abnormalities of the surface charge, etc., for subsequent development of methods for early diagnostics of cancer based on laser light scattering and fluorescence. Materials and methods: The parameters of protein dynamics were assessed by laser light scattering methods. Results: We performed comparative assessments in model systems and native serum samples for cancer diagnostics and analyzed the possibility to use optical parameters of water serum solutions for development of cancer diagnostic methods. Conclusion: Light scattering methods provide sufficient information on changes of the static and dynamic properties of the serum proteins over time during various pathological conditions