7 research outputs found
Not Available
No full textNot AvailableThe present study was undertaken to assess the effect of melatonin (MT) on sperm motility, viability, total sperm abnormality, acrosomal and plasma membrane integrity, DNA abnormality, antioxidant profiles such as superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and total antioxidant capacity (TAC), enzymatic profiles such as aspartate amino transaminase (AST), alanine amino transaminase (ALT), and biochemical profiles such as malonaldehyde (MDA) production and cholesterol efflux. Total numbers of 30 ejaculates were collected twice a week from eight mithun bulls and semen was split into five equal aliquots, diluted with the TEYC extender. Group 1 has semen without additives (control) and group 2 to group 5 have semen that was diluted with 1mM, 2mM, 3mM, and 4mMof melatonin, respectively.These seminal parameters, antioxidant, enzymatic, and biochemical profiles were assessed at 5∘C for 0, 6, 12, 24, and 30 h of incubation. Inclusion of melatonin into diluent resulted in significant ( < 0.05) decrease in percentages of dead spermatozoa, abnormal spermatozoa, and acrosomal abnormalities at different hours of storage periods as compared with control group. Additionally,melatonin at 3mMhas significant improvement in quality ofmithun semen than melatonin at 1mM, 2mMor 4mMstored in in vitro for up to 30 h. It was concluded that the possible protective effects
of melatonin on spermparameters are it preventsMDA production and preserve the antioxidants and intracellular enzymes during preservation.Not Availabl
Not Available
No full textNot AvailableMithun (Bos frontalis) is mostly utilized for
meat purpose by the local tribal people of north eastern hilly
region of India. Meat born diseases are very important issues
for the people of these areas from the public health point of
view. In order to realize the issues, the serological prevalence
of Toxoplasmosis was carried out as per ELISA kit
(CHEKIT* TOXOTEST, IDEXX Laboratory, USA) to
find out Toxoplasma gondii antibody. As a whole, out of
195 animals examined, only 8 (4.10 %) animals were found
to be suspected and one (0.51 %) animal was found to be
positive in this precious animal of the north eastern region.Not Availabl
Not Available
No full textNot AvailableThe present study was undertaken to assess the effect of bovine serum albumin (BSA)
on sperm motility, viability, total spermabnormality, acrosomal and plasmamembrane integrity.
A total of 30 ejaculates were collected from mithun bulls and semen was split into five equal
aliquots, diluted with the TEYC extender. Group 1: semen without additives (control), group 2 to
group 4: semen was diluted with 5 mg/ml, 10 mg/ml and 20 mg/ml of BSA, respectively. These
seminal parameters were assessed at 5oC for 0, 6, 12, 24 and 30 h of incubation. Inclusion of
BSA into diluent resulted in significant (P <0.05) decrease in percentages of dead spermatozoa,
abnormal spermatozoa and acrosomal abnormalities at different hours of storage periods as
compared with control group.Additionally, BSAat 5mg/ml has significant (p< 0.05) improvement
in quality of semen than BSA at 10 mg/ml and 20 mg/ml stored in in-vitro for up to 30 h. It was
concluded that the possible protective effects of BSA on sperm parameters may be due to the
prevention of lipid peroxidation.Not Availabl
