Genetic enhancement of okra [Abelmoschus esculentus (L.) Moench] germplasm through wide hybridization

IntroductionThe introgression of genetic material from one species to another through wide hybridization and repeated back-crossing, plays an important role in genetic modification and enriching the cultivated gene-pool with novel genetic variations. Okra (Abelmoschus esculentus [(L.) Moench)] is a popular vegetable crop with high dietary fibre and protein, rich in essential amino acids, lysine and tryptophan. The wild Abelmoschus genepool has many desirable traits like ornamental value, short internodal length, more number of productive branches, extended bearing, perennation tendency, reduced fruit length (more consumer preferred trait), high mucilage content (medicinal value), abiotic stress tolerances such as drought, high temperature and biotic stress resistances such as okra Yellow Vein Mosaic Virus (YVMV) and Enation Leaf Curl Virus (ELCV) diseases. The repeated use of elite breeding lines led to narrowing of the genetic base of the okra crop, one of the major factors attributed to breakdown of resistance/ tolerance to biotic stresses. YVMV and ELCV are the two major diseases, causing significant yield loss in okra. Hence, wide hybridization was attempted to transfer tolerance genes from wild species to the cultivated genepool to widen the genetic base.Material and methodsThe screening of germplasm of wild Abelmoschus species at hotspots led to the identification of tolerant species (Abelmoschus pungens var. mizoramensis, A. enbeepeegeearensis, A. caillei, A. tetraphyllus and A. angulosus var. grandiflorus), which were further used in a wide-hybridization programme to generate interspecific hybrids with the cultivated okra. Presence of pre- and post-zygotic barriers to interspecific geneflow, differences in ploidy levels and genotype specific variations in chromosome numbers led to varying degrees of sterility in F1 plants of interspecific crosses. This was overcome by doubling the chromosome number of interspecific hybrids by applying Colchicine at the seedling stage. The 113 cross derivatives generated comprising amphidiploids in the F1 generation (30), F3 (14), one each in F2 and F4 generations, back cross generation in BC1F2 (03), BC1F3 (25), and BC2F3 (02), crosses between amphidiploids (27), multi-cross combinations (07) and inter-specific cross (between A. sagittifolius × A. moschatus subsp. moschatus) selfed derivatives at F8 generation (03) were characterized in the present study. Besides they were advanced through selfing and backcrossing.Results and DiscussionThe amphidiploids were found to possess many desirable genes with a considerable magnitude of linkage drag. Majority of the wide cross derivatives had an intermediate fruit morphology and dominance of wild characters viz., hispid fruits, stem, leaves, tough fruit fibre, vigorous perennial growth habit and prolonged flowering and fruiting. The fruit morphology of three BC progenies exhibited a high morphological resemblance to the cultivated okra, confirming successful transfer of useful genes to the cultivated okra genepool. The detailed morphological characteristics of the various combinations of Abelmoschus amphidiploids and the genetic enhancement of the genepool achieved in this process is reported here

Atmospheric boundary layer characteristics during the BOBMEX-pilot experiment

The atmospheric boundary layer characteristics observed during the BOBMEX-Pilot experiment are reported. Surface meteorological data were acquired continuously through an automatic weather monitoring system and manually every three hours. High resolution radiosondes were launched to obtain the vertical thermal structure of the atmosphere. The study area was convectively active, the SSTs were high, surface air was warm and moist, and the surface air moist static energy was among the highest observed over the tropical oceans. The mean sea air temperature difference was about 1.25 degrees C and the sea skin temperature was cooler than bucket SST by 0.5 degrees C. The atmospheric mixed layer was shallow, fluctuated in response to synoptic conditions from 100 m to 900 m with a mean around 500 m

DNA fingerprinting of Musa cultivars with oligodeoxyribonucleotide probes specific for simple repeat motifs

Using synthetic oligodeoxyribonucleotide probes against restriction-digested genomic DNA, we have established DNA fingerprinting of Musa cultivars. Of all the enzymes used, Eco RI and Hin dIII were found to be most informative, giving rise to individual specific band patterns with oligonucleotide probes of 15- to 18-base residues. Of the several probes and enzyme combinations used, the 15mer GACA probe with Eco RI and Hin dIII digests revealed a maximal level of polymorphism, and the probability of obtaining an identical band pattern between any two random genotypes was calculated to be 1.50 × 10-9 and 1.59 × 10-9, respectively. Oligonucleotide probes longer than 22 residues were also used but did not hybridize. The present approach is useful for cultivar identification and for overall genome analysis to establish relatedness among the various accessions of the Musa germ-plasm originating from different geographic locations. The relevance of using synthetic oligonucleotide probes based on simple repeat motifs for achieving DNA fingerprinting pattern is discussed

Inertial-Dissipation flux measurements over south Bay of Bengal during BOBMEX-Pilot experiment

This paper describes measurement of air-sea parameters and estimation of sensible and latent heat fluxes by the "Inertial- Dissipation" technique over south Bay of Bengal. The data were collected on ORV Sagar Kanya during BOBMEX-Pilot cruise during the period 23rd October 1998 to 12th November 1998 over south Bay of Bengal. The fluxes are estimated using the data collected through fast response sensors namely Gill anemometer, Sonic anemometer and IR Hygrometer. In this paper the analyses carried out for two days, one relatively cloud free day on November 3rd and the other cloudy with rain on November Ist, are presented. Sea surface and air temperatures are higher on November 3rd than on November Ist. Sensible heat flux for both the days does not show any significant variation over the period of estimation, whereas latent heat flux is more for November 3rd than November Ist. An attempt is made to explain the variation of latent heat flux with a parameter called thermal stability on the vapor transfer from the water surface, which depends on wind speed and air to sea surface temperature difference