3,357 research outputs found
Ward Identities in Non-equilibrium QED
We verify the QED Ward identity for the two- and three -point functions at
non-equilibrium in the HTL limit. We use the Keldysh formalism of real time
finite temperature field theory. We obtain an identity of the same form as the
Ward identity for a set of one loop self-energy and one loop three-point vertex
diagrams which are constructed from HTL effective propagators and vertices.Comment: 19 pages, RevTex, 4 PostScript figures, revised version to be
published in Phys. Rev.
The tax-inducible actin-bundling protein fascin is crucial for release and cell-to-cell transmission of human T-cell leukemia virus type 1 (HTLV-1)
The delta-retrovirus Human T-cell leukemia virus type 1 (HTLV-1) preferentially infects CD4(+) T-cells via cell-to-cell transmission. Viruses are transmitted by polarized budding and by transfer of viral biofilms at the virological synapse (VS). Formation of the VS requires the viral Tax protein and polarization of the host cytoskeleton, however, molecular mechanisms of HTLV-1 cell-to-cell transmission remain incompletely understood. Recently, we could show Tax-dependent upregulation of the actin-bundling protein Fascin (FSCN-1) in HTLV-1-infected T-cells. Here, we report that Fascin contributes to HTLV-1 transmission. Using single-cycle replication-dependent HTLV-1 reporter vectors, we found that repression of endogenous Fascin by short hairpin RNAs and by Fascin-specific nanobodies impaired gag p19 release and cell-to-cell transmission in 293T cells. In Jurkat T-cells, Tax-induced Fascin expression enhanced virus release and Fascin-dependently augmented cell-to-cell transmission to Raji/CD4(+) B-cells. Repression of Fascin in HTLV-1-infected T-cells diminished virus release and gag p19 transfer to co-cultured T-cells. Spotting the mechanism, flow cytometry and automatic image analysis showed that Tax-induced T-cell conjugate formation occurred Fascin-independently. However, adhesion of HTLV-1-infected MT-2 cells in co-culture with Jurkat T-cells was reduced upon knockdown of Fascin, suggesting that Fascin contributes to dissemination of infected T-cells. Imaging of chronically infected MS9 T-cells in co-culture with Jurkat T-cells revealed that Fascin's localization at tight cell-cell contacts is accompanied by gag polarization suggesting that Fascin directly affects the distribution of gag to budding sites, and therefore, indirectly viral transmission. In detail, we found gag clusters that are interspersed with Fascin clusters, suggesting that Fascin makes room for gag in viral biofilms. Moreover, we observed short, Fascin-containing membrane extensions surrounding gag clusters and clutching uninfected T-cells. Finally, we detected Fascin and gag in long-distance cellular protrusions. Taken together, we show for the first time that HTLV-1 usurps the host cell factor Fascin to foster virus release and cell-to-cell transmission
Autowaves in a dc complex plasma confined behind a de Laval nozzle
Experiments to explore stability conditions and topology of a dense
microparticle cloud supported against gravity by a gas flow were carried out.
By using a nozzle shaped glass insert within the glass tube of a dc discharge
plasma chamber a weakly ionized gas flow through a de Laval nozzle was
produced. The experiments were performed using neon gas at a pressure of 100 Pa
and melamine-formaldehyde particles with a diameter of 3.43 {\mu}m. The
capturing and stable global confining of the particles behind the nozzle in the
plasma were demonstrated. The particles inside the cloud behaved as a single
convection cell inhomogeneously structured along the nozzle axis in a tube-like
manner. The pulsed acceleration localized in the very head of the cloud
mediated by collective plasma-particle interactions and the resulting wave
pattern were studied in detail.Comment: 6 pages, 4 figure
Emittance growth in linear induction accelerators
The Dual-Axis Radiographic Hydrotest (DARHT) facility uses bremsstrahlung
radiation source spots produced by the focused electron beams from two linear
induction accelerators (LIAs) to radiograph large hydrodynamic experiments
driven by high explosives. Radiographic resolution is determined by the size of
the source spot, and beam emittance is the ultimate limitation to spot size. On
the DARHT Axis-II LIA we measure an emittance higher than predicted by
theoretical simulations, and even though this axis produces sub-millimeter
source spots, we are exploring ways to improve the emittance. Some of the
possible causes for the discrepancy have been investigated using
particle-in-cell (PIC) codes, although most of these are discounted based on
beam measurements. The most likely source of emittance growth is a mismatch of
the beam to the magnetic transport, which can cause beam halo.Comment: 20th Int. Conf. on High-Power Particle Beams, Washington, DC, May,
201
Bremsstrahlung from an Equilibrating Quark-Gluon Plasma
The photon production rate from a chemically equilibrating quark-gluon plasma
likely to be produced at RHIC (BNL) and LHC (CERN) energies is estimated taking
into account bremsstrahlung. The plasma is assumed to be in local thermal
equilibrium, but with a phase space distribution that deviates from the Fermi
or Bose distribution by space-time dependent factors (fugacities). The photon
spectrum is obtained by integrating the photon rate over the space-time history
of the plasma, adopting a boost invariant cylindrically symmetric transverse
expansion of the system with different nuclear profile functions. Initial
conditions obtained from a self-screened parton cascade calculation and, for
comparison, from the HIJING model are used. Compared to an equilibrated plasma
at the same initial energy density, taken from the self-screened parton
cascade, a moderate suppression of the photon yield by a factor of one to five
depending on the collision energy and the photon momentum is observed. The
individual contributions to the photon production, however, are completely
different in the both scenarios.Comment: 14 pages, 4 figures, shortened version to be published in Phys. Rev.
Parton Equilibration in Relativistic Heavy Ion Collisions
We investigate the processes leading to phase-space equilibration of parton
distributions in nuclear interactions at collider energies. We derive a set of
rate equations describing the chemical equilibration of gluons and quarks
including medium effects on the relevant QCD transport coefficients, and
discuss their consequences for parton equilibration in heavy ion collisions.Comment: 18 pages, 6 Figures appended as uuencoded PostScript files, (no
changes in the previously submitted manuscript), DUKE-TH-93-4
First Results from Viper: Detection of Small-Scale Anisotropy at 40 GHZ
Results of a search for small-scale anisotropy in the cosmic microwave
background (CMB) are presented. Observations were made at the South Pole using
the Viper telescope, with a .26 degree (FWHM) beam and a passband centered at
40 GHz. Anisotropy band-power measurements in bands centered at l = 108, 173,
237, 263, 422 and 589 are reported. Statistically significant anisotropy is
detected in all bands.Comment: 5 pages, 4 figures, uses emulateapj.sty, submitted to ApJ Letter
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