SCREENING AND BIOSYTNTHESIS OF FIBRINOLYTIC ENZYME FROM ASPERGILLUS JAPONICUM

Fibrin is a protein that forms in the blood clots after trauma or injury. This is essential to stop blood loss. There are more than twenty enzymes in the body that assist in clotting of the blood, while only one that can break the clot down. It is an endogenously produced fibrinolytic enzyme called plasmin. Streptokinase is an extracellular metallo-enzyme produced by beta-haemolytic streptococcus and is used as an effective and cheap clot-dissolving medication in some cases of myocardial infarction (heart attack) and pulmonary embolism. It belongs to a group of medications known as fibrinolytics. Fibrinolytic enzymes can be found in a variety of foods, such as Japanese Natto, Tofuyo, Korean Chungkook-Jang soy sauce, and edible honey mushroom. Fibrinolytic enzymes producing Aspergillus japonicum KSS 05 strain were screened for the production by fibrin plate assay method. The maximum zone of fibrin hydrolysis were found 6 mm diameter. Further the Aspergillus japonicum KSS 05 were employed for the production by submerged fermentation and it showed 235 IU by pH 6, temperature 300C and 1 ml inoculums size. Key words: Fermentation kinetics, effect of pH, fibrinolytic enzymes and fibrinÂ

EFFECT OF FERMENTATION KINETICS FOR THE BIOSYNTHESIS OF PROTEASE FROM ASPERGILLUS AWAMORI

Among the various groups of microorganisms filamentous fungi are most widely exploited because of their ability to grow on complex medium and production of wide range of extracellular enzymes. This study highlights the production of extracellular protease biosynthesis were carried out by using Aspergillus awamori was evaluated under different fermentation kinetics by employing submerged fermentation method. The protease producers detected by the clear zone (casein hydrolysis) around the colony by simple plate assay method. Aspergillus awamori KGSR 12 was the potential strain among the fungal isolates. The Protease biosynthesis was increased their yield after the optimization of fermentation parameters. The optimum pH 6.0 (1.96 IU), temperature 350C (2.12 IU) and inoculum size 0.5 ml showed 2.23 IU. Key words: Fermentation Kinetics, Aspergillus awamori, Protease, Plate assay. Key words: Fermentation Kinetics, Aspergillus tamarii, Protease, Plate assay

MOLECULAR CONFIRMATION, IDENTIFICATION AND INFLUENCE OF CARBON SOURCE FOR THE PRODUCTION OF XYLANASE FROM PENICILLIUM CITRINUM

Recently, xylanases have expanded their use in many processing industries, such as pulp and paper, food and textile to newer needs such as biofuel production. .This study were taken up to the enhance the biosynthesis of xylanase by supplementation of carbon sources were employed in range of 0.25% to 1.0%. The carbon source were supplemented are glucose, sucrose and maltose. The beef extract and ammonium nitrate were yielded higher xylnase production and showed 7.5 IU and 8.4 IU.Key words: Xylanase, submerged fermentation, xylose, fermentation kinetics and inoculums size

EFFECT OF NITROGEN SOURCE FOR THE BIOSYNTHESIS OF XYLANASE FROM ASPERGILLUS TAMARII

The main aim of the study is to enhance the biosynthesis of xylanse by incorporating nitrogen sources (both organic and inorganic). Recently, xylanases have expanded their use in many processing industries, such as pulp and paper, food and textile to newer needs such as biofuel production. .This study were taken up to the enhance the biosynthesis of xylanase by supplementation of organic nitrogen and inorganic nitrogen sources were employed in range of 0.25% to 1.25%. The organic nitrogen source were supplemented are peptone, yeast extract and beef extract and inorganic nitrogen sources are ammonium sulphate, ammonium chloride and ammonium nitrate. The yeast extract and ammonium nitrate were yielded higher xylnase production and showed 5.56 IU and 5.79 IU Key words: Xylanase, submerged fermentation, yeast extract, ammonium nitrate and assay

ROLE OF CARBON SOURCE FOR THE PRODUCTION OF L-GLUTAMINASE FROM ASPERGILLUS ORYZAE

Microbial glutaminases are more stable than plant and animal counterparts. In addition to it, they have also been detected in mammalian tissues where they are the major enzymes responsible for catabolic breakdown of glutamine. This study were taken up to the enhance the biosynthesis of L-glutaminase by supplementation of carbon sources were employed in range of 0.25% to 1.0%. The carbon source were supplemented are glucose, fructose and lactose. In case of monosaccharides (fructose) the maximum L-glutaminase production of 494.1 IU was observed at 1% and where as disaccharides like lactose yielded maximum L-glutaminase of 343.5 IU.Key words: L-glutaminase, submerged fermentation, fructose, Aspergillus oryzaeÂ

ROLE OF NITROGEN SOURCE FOR THE PRODUCTION OF XYLANASE FROM ASPERGILLUS SP

Recently, xylanases have expanded their use in many processing industries, such as pulp and paper, food and textile to newer needs such as biofuel production. .This study were taken up to the enhance the biosynthesis of xylanase by supplementation of organic nitrogen and inorganic nitrogen sources were employed in range of 0.25% to 1.0%. The organic nitrogen source were supplemented are peptone, yeast extract and beef extract and inorganic nitrogen sources are ammonium Sulphate and ammonium chloride. The beef extract and ammonium nitrate were yielded higher xylnase production and showed 7.5 IU and 8.46 IU Key words: Xylanase, submerged fermentation, xylose, fermentation kinetics and inoculums siz

ROLE OF NITROGEN SOURCE FOR THE PRODUCTION OF XYLANASE FROM ASPERGILLUS SP

Recently, xylanases have expanded their use in many processing industries, such as pulp and paper, food and textile to newer needs such as biofuel production. .This study were taken up to the enhance the biosynthesis of xylanase by supplementation of organic nitrogen and inorganic nitrogen sources were employed in range of 0.25% to 1.0%. The organic nitrogen source were supplemented are peptone, yeast extract and beef extract and inorganic nitrogen sources are ammonium Sulphate and ammonium chloride. The beef extract and ammonium nitrate were yielded higher xylnase production and showed 7.5 IU and 8.46 IU Key words: Xylanase, submerged fermentation, xylose, fermentation kinetics and inoculums siz

AN APPROACH ISOLATION,SCREENING AND PRODUCTION OF PROTEASE FROM ASPERGILLUS ORYZAE

This study investigates the production of extracellular protease synthesis were carried out by using Aspergillusoryzae was evaluated under different fermentation parameters by employing submerged fermentation method. The protease producers detected by the clear zone (casein hydrolysis) around the colony by simple plate assay method. AspergillusoryzaeKS 5 is the potential strain among the fungal isolates. The Proteasesynthesis were increased their yield after the optimization of fermentation parameters. The optimum pH 6.0, temperature 350C and inoculum size 1.0 ml and it showed 1.7 IU.Key words: Protease, Plate assay, Optimization and Aspergillusoryza

INVESTIGATION ON THE PRODUCTION OF L-GLUTAMINASE FROM PSEUDOMONAS STUTZERI STRAIN UNDER SOLID STATE FERMENTATION USING VARIOUS AGRO RESIDUES

Solid state fermentation was carried out for the production of L-glutaminase by Pseudomonas stutzeri PIMS6 using different agro residues including green gram husk, Bengal gram husk, cattle feed, wheat bran and groundnut oil cake as solid substrates. L-glutaminase has received significant attention in recent years owing to its potential applications in medicine as an anticancer agent, as an efficient anti-retroviral agent and as a biosensor. In food industries it is used as a flavor and aroma enhancing agent. The maximum yield (55.24 U/gds) of L-glutaminase by Pseudomomonas stutzeri PIMS6 was obtained using cattle feed at 75% initial moisture content, initial pH 8.0, supplemented with glucose (1.0%), ammonium sulphate (1.0%),  inoculated with 5% of inoculum and incubated at 37°C for 96 h. Both physico-chemical and nutritional parameters played a significant role in the production of the enzyme L-glutaminase.Keywords: L-glutaminase, Pseudomonas stutzeri PIMS6, Cattle feed, Solid state fermentation

ISOLATION, SCREENING AND PRODUCTION OF XYLANASE FROM ASPERGILLUS SP

ABSTRACT Recently, xylanases have expanded their use in many processing industries, such as pulp and paper, food and textile to newer needs such as biofuel production.This study investigates the production of extracellular xylanase synthesis were carried out by using Aspergillus sp. were evaluated under different fermentation parameters by employing submerged fermentation method. The xylanase producers detected by the enzyme hydrolytic zone around the colony by simple plate assay method. Aspergillus sp. 05 is the potential strain among the fungal isolates. The xylanase synthesis were increased their yield after the optimization of fermentation parameters. The optimum pH 6.0, temperature 30 º C and inoculum size 0.75 ml and it showed 5.53 IU