Binary Logistic Regression Models Enable Mirna Profiling To Provide Accurate Identification Of Forensically Relevant Body Fluids And Tissues

Numerous studies have demonstrated the ability to identify the body fluid of origin of forensic biological stains using messenger (mRNA) profiling. However, the size of the amplification product used in these assays (100-400 bases) may not be ideal for use with environmentally degraded samples. MiRNA profiling represents a potential alternative to mRNA profiling, since the small size of the miRNAs (~22 bases) might still permit their detection in degraded stains. Previously, we reported the first study involving the forensic use of microRNA (miRNA) profiling, which required screening of 452 candidates. Since our initial screening, hundreds of novel miRNAs have been identified. We have therefore evaluated additional miRNA candidates to further improve the sensitivity and specificity of the body fluid assays. Consequently we have expanded our body fluid identification panel to include 18 miRNAs (comprising 5 original and 13 novel miRNAs). This panel permits the identification of all forensically relevant body fluids and, uniquely, includes miRNAs for the identification of skin. Using normalized miRNA expression data, we constructed body fluid specific binary logistic regression models to permit an accurate identification of the body fluid of interest. Using the developed models, we have obtained 100% accuracy in predicting the body fluid of interest. © 2013 Elsevier Ireland Ltd

The Identification Of Menstrual Blood In Forensic Samples By Logistic Regression Modeling Of Mirna Expression

We report the identification of sensitive and specific miRNA biomarkers for menstrual blood, a tissue that might provide probative information in certain specialized instances. We incorporated these biomarkers into qPCR assays and developed a quantitative statistical model using logistic regression that permits the prediction of menstrual blood in a forensic sample with a high, and measurable, degree of accuracy. Using the developed model, we achieved 100% accuracy in determining the body fluid of interest for a set of test samples (i.e. samples not used in model development). The development, and details, of the logistic regression model are described. Testing and evaluation of the finalized logistic regression modeled assay using a small number of samples was carried out to preliminarily estimate the limit of detection (LOD), specificity in admixed samples and expression of the menstrual blood miRNA biomarkers throughout the menstrual cycle (25-28 days). The LOD was \u3c1 ng of total RNA, the assay performed as expected with admixed samples and menstrual blood was identified only during the menses phase of the female reproductive cycle in two donors

Photodegradation of the radionuclide complexants

SSCI-VIDE+CARE+CGU:FDAInternational audienceThe degradation and mineralization of radionuclide complexants in nuclear liquid waste was studied by using different Advanced Oxidation Processes (AOPs). Three complexants of radionuclide were chosen for this study, tributylphosphate (TBP), dibutylphosphate (DBP) and ethylenediaminetetraacetic acid (EDTA). The efficiency of photocatalytic methods based on TiO2 (Evonik P-25) as means of degrading, mineralizing, TBP, DBP and EDTA were studied under UV-A and UV-C irradiation. In the case of EDTA, we also compared the efficiency of the photocatalytic process TiO2/UV-C and of the photolysis of H2O2 (H2O2 /UV-C) of EDTA alone and complexes with cobalt as radioelement and studied the recovery of the cobalt. In addition to HPLC, GC and TOC analysis allowing to determine the disappearance and the mineralization rates and the intermediate products formed, 31P NMR was used to follow the fate of phosphorus atom. Our results show that DBP is an intermediate product of the degradation of TBP and that the same types of carboxylic acids are formed during the degradation of TBP and DBP compounds. We also show that the kinetics of the mineralization process is described in both cases by the Langmuir-Hinshelwood model, with identical rate constants. The experiments realized with 59Co-EDTA allowed to highlight the efficiency of the UV/TiO2 and UV/H2O2 processes and to optimize them. This degradation is accompanied by an important mineralization of carbon and nitrogen initially present in EDTA and the intermediate formation of smaller molecules such as acetic acid and oxalic acids. Once separated from organic ligands, cobalt can afterwards be trapped in mineral materials in view of its storage. The efficiency of the UV/TiO2 and UV/H2O2 processes was found to be similar, provided H2O2 was added continuously during the UV/H2O2 process

Organometallic compound degradation by Advanced Oxidation Processes

Internationa

degradation of cobalt(ii)-edta complex using aops. application at the nuclear field

SSCI-VIDE+CARE+CGUInternational audienceNon

Photocatalysis and H2O2/UV-C - Application to nuclear wastes

SSCI-VIDE+CARE+CGU:FDA:JMHInternational audienceThe efficiency of photocatalytic methods based on TiO2 (Degussa P-25) and UV/H2O2 as means of degrading, mineralizing Ethylenediaminetetraacetic acid (EDTA) alone or complexes with radioelement and recovering the radioelement (Co) were studied under UV-C irradiation. EDTA was chosen because it is present in high proportions at nuclear facilities, where it is used for cleaning and decontamination purposes because of its chelating properties. In a first step the photonic flux of the UV-C lamps was established using actinometric and Keitzâs methods. Then adsorption capacities of TiO2 to adsorb Co complexes or not with EDTA have been studied. Finally, we compared the disappearance and mineralization rates, the intermediate by-products formed in the degradation of EDTA under UV-C photolysis, photocatalysis and H2O2/UV-C and studiedthe effect of pH. The results show that the efficiency of the UV/TiO2 and UV/H2O2 processes was found to be similar, provided H2O2 was added continuously during the UV/H2O2 process. The photonic efficiency was calculated and found to be equal to 0.9% in the case of photocatalysis and 1.4% in that of UV/H2O

Photodegradation of the radionuclide complexants

SSCI-VIDE+CARE+CGU:FDAInternational audienceThe degradation and mineralization of radionuclide complexants in nuclear liquid waste was studied by using different Advanced Oxidation Processes (AOPs). Three complexants of radionuclide were chosen for this study, tributylphosphate (TBP), dibutylphosphate (DBP) and ethylenediaminetetraacetic acid (EDTA). The efficiency of photocatalytic methods based on TiO2 (Evonik P-25) as means of degrading, mineralizing, TBP, DBP and EDTA were studied under UV-A and UV-C irradiation. In the case of EDTA, we also compared the efficiency of the photocatalytic process TiO2/UV-C and of the photolysis of H2O2 (H2O2 /UV-C) of EDTA alone and complexes with cobalt as radioelement and studied the recovery of the cobalt. In addition to HPLC, GC and TOC analysis allowing to determine the disappearance and the mineralization rates and the intermediate products formed, 31P NMR was used to follow the fate of phosphorus atom. Our results show that DBP is an intermediate product of the degradation of TBP and that the same types of carboxylic acids are formed during the degradation of TBP and DBP compounds. We also show that the kinetics of the mineralization process is described in both cases by the Langmuir-Hinshelwood model, with identical rate constants. The experiments realized with 59Co-EDTA allowed to highlight the efficiency of the UV/TiO2 and UV/H2O2 processes and to optimize them. This degradation is accompanied by an important mineralization of carbon and nitrogen initially present in EDTA and the intermediate formation of smaller molecules such as acetic acid and oxalic acids. Once separated from organic ligands, cobalt can afterwards be trapped in mineral materials in view of its storage. The efficiency of the UV/TiO2 and UV/H2O2 processes was found to be similar, provided H2O2 was added continuously during the UV/H2O2 process