Biochemical and molecular study in diabetic infertile males

As per the statement by World Health Organization, Infertility is presence of a medical condition of the couple with inability in getting conceived for 1 year or more without usage of any contraception. In the present situation, the incidence of Infertility has increased and it is viewed as a major concern with affecting about 15% to 20% of the couples. A detailed systematic examination of history, investigative protocol is necessary for proper management of infertility. The patients were also advised for blood hormonal analysis tests such as Follicle Stimulation Hormone (FSH), Testosterone, Luteinizing Hormone (LH), Prolactin. Methylene tetrahydrofolate reductase (MTHFR) and methionine synthase reductase (MTRR) are the key enzymes in folate metabolism. The enzyme activities of MTHFR and MTRR are influenced by gene polymorphisms. So the polymorphisms of MTHFR and MTRR may be a potential risk factor for male infertility. The present study was done in 100 males, 50 diabetic infertile and 50 non-diabetic fertile men. Semen samples were obtained and analyzed as per the instructions given in the WHO manual for semen analysis [10]. A blood sample was collected and analyzed for the levels of FBS, FSH, LH, Prolactin and Testosterone

Study of biochemical and sperm parameters in infertile males with diabetes mellitus

World Health Organization has stated Infertility as a medical condition in which an individual has the inability to conceive even after one year of regular unprotected active sexual intercourse. Presently, Infertility has been a major condition, which is multifactorial in nature and is supposed to have been affected approximately about 15% to 20% of the couples who are desperate for pregnancy. The contributory factors for male infertility may be of different factors comprising of complex pathogenesis, change in lifestyle, other organic diseases, genetic factors, environmental risk factors and their interactions. The patients with subnormal semen parameters are advised for blood hormonal analysis tests as these hormonal tests are more reliable and valid in the further proper management of the infertility [11]. These hormonal tests include Follicle Stimulation Hormone (FSH), Testosterone, Luteinizing Hormone (LH), Prolactin. This cross section study was done in 100 males, 50 diabetic infertile men and 50 non-diabetic fertile men. Semen samples were obtained by masturbation after 2–7 days of sexual abstinence. The Semen Samples were analyzed as per the instructions given in the WHO manual for semen analysis.&nbsp