Fast Growth Increases the Selective Advantage of a Mutation Arising Recurrently during Evolution under Metal Limitation

Understanding the evolution of biological systems requires untangling the molecular mechanisms that connect genetic and environmental variations to their physiological consequences. Metal limitation across many environments, ranging from pathogens in the human body to phytoplankton in the oceans, imposes strong selection for improved metal acquisition systems. In this study, we uncovered the genetic and physiological basis of adaptation to metal limitation using experimental populations of Methylobacterium extorquens AM1 evolved in metal-deficient growth media. We identified a transposition mutation arising recurrently in 30 of 32 independent populations that utilized methanol as a carbon source, but not in any of the 8 that utilized only succinate. These parallel insertion events increased expression of a novel transporter system that enhanced cobalt uptake. Such ability ensured the production of vitamin B12, a cobalt-containing cofactor, to sustain two vitamin B12–dependent enzymatic reactions essential to methanol, but not succinate, metabolism. Interestingly, this mutation provided higher selective advantages under genetic backgrounds or incubation temperatures that permit faster growth, indicating growth-rate–dependent epistatic and genotype-by-environment interactions. Our results link beneficial mutations emerging in a metal-limiting environment to their physiological basis in carbon metabolism, suggest that certain molecular features may promote the emergence of parallel mutations, and indicate that the selective advantages of some mutations depend generically upon changes in growth rate that can stem from either genetic or environmental influences

Hard X-Ray Pulsations In Gx 1+4

The x-ray pulsar GX 1+4 was observed by us in four balloon- borne experiments carried out from Hyderabad, India during 1991-1995 period with a hard x-ray telescope. The x-ray telescope consists of two collimated large area xenon-filled proportional counters with an effective area of 2400 cm 2 , a field of view of 5 ffi \Theta 5 ffi and sensitive in the energy band of 20 - 100 keV. The pulsar was detected in bright state in two of the four experiments and x-ray pulsations with 120 second period were detected clearly. Pulsation period, rate of change of period with time, pulse fraction, pulse profile and energy spectra of the source were determined from these studies. During March 1995 observation, the x-ray pulse of GX 1+4 was found to be double-peaked compared to a single-peak pulse profile detected in December 1993. Details of these results are presented and their interpretation discussed in terms of the current accretion models of x-ray binaries. I. INTRODUCTION The 121 sec x..

Effects of Mutations in the Pseudomonas putida miaA Gene: Regulation of the trpE and trpGDC Operons in P. putida by Attenuation

Tn5 insertion mutants defective in regulation of the Pseudomonas putida trpE and trpGDC operons by tryptophan were found to contain insertions in the P. putida miaA gene, whose product (in Escherichia coli) modifies tRNA(Trp) and is required for attenuation. Nucleotide sequences upstream of trpE and trpG encode putative leader peptides similar in sequence to leader peptides found in other bacterial species, and the phenotypes of the mutants strongly suggest that transcription of these operons is regulated solely by attenuation