Uterine preservation with Cho suture in localized multifocal atonicity of uterus with failed medical management and uterine tamponade

Postpartum haemorrhage (PPH) is a major cause of maternal morbidity and mortality, and one of the common obstetrical emergencies. Quite commonly, it occurs in patients where PPH is not expected. Management has to be swift and precise according to the steps and the response initiated by the uterine musculature during the management. In our patient, we had a localized multifocal atony of the uterus, at points where the uterine sinuses were bleeding due to atony, and medical management and uterine tamponade failed, with a raised D-dimer level. As the stepwise management failed with uterine devascularisation failing to control PPH, a decision for uterine compression suture (UCS), Cho suture was taken

Rough triggerfish, Canthidermis maculata from Gujarat coast

A specimen of the Rough triggerfish, Canthidermis maculata (Bloch, 1786) was landed by a multiday trawler on 25th November 2015 at Mangrol Fisheries Harbour, Gujarat. The specimen was brownish grey in colour with characteristic white spots all over the body including the head. It measured 309 mm and 256 mm in Total length and Standard length respectively with a weight of 580 g (Fig. 1). The fish is usually found in areas having sandy, muddy or rocky bottoms at a depth range of 50-100 m and is reported from the Western Indian Ocean

Endogenous transforming growth factor β1 suppresses inflammation and promotes survival in adult CNS

Transforming growth factor β1 (TGFβ1) is a pleiotropic cytokine with potent neurotrophic and immunosuppressive properties that is upregulated after injury, but also expressed in the normal nervous system. In the current study, we examined the regulation of TGFβ1 and the effects of TGFβ1 deletion on cellular response in the uninjured adult brain and in the injured and regenerating facial motor nucleus. To avoid lethal autoimmune inflammation within 3 weeks after birth in TGFβ1-deficient mice, this study was performed on a T- and B-cell-deficient RAG2-/- background. Compared with wild-type siblings, homozygous deletion of TGFβ1 resulted in an extensive inflammatory response in otherwise uninjured brain parenchyma. Astrocytes increased in GFAP and CD44 immunoreactivity; microglia showed proliferative activity, expression of phagocytosis-associated markers [αXβ2, B7.2, and MHC1 (major histocompatibility complex type 1)], and reduced branching. Ultrastructural analysis revealed focal blockade of axonal transport, perinodal damming of axonal organelles, focal demyelination, and myelin debris in granule-rich, phagocytic microglia. After facial axotomy, absence of TGFβ1 led to a fourfold increase in neuronal cell death (52 vs 13%), decreased central axonal sprouting, and significant delay in functional recovery. It also interfered with the microglial response, resulting in a diminished expression of early activation markers [ICAM1 (intercellular adhesion molecule 1), α6β1, and αMβ2] and reduced proliferation. In line with axonal and glial findings in the otherwise uninjured CNS, absence of endogenous TGFβ1 also caused an ∼10% reduction in the number of normal motoneurons, pointing to an ongoing and potent trophic role of this anti-inflammatory cytokine in the normal as well as in the injured brain. Copyright © 2007 Society for Neuroscience

Development and Validation of Analytical methods for estimation of Simvastatin and Fenofibrate

The present work describes Stability indicating RP-HPLC and First order derivative UV spectrophotometric method for the quantitative determination of Simvastatin and Fenofibrate. Materials and methods: The parameters Specificity, linearity, accuracy, precision, detection limit, quantitation limit, Robustness and system suitability tests were studied and their results were compiled to ICH guideline Q2 (R2).  Chromatography was carried out by reverse phase technique on an RP-18 with mobile phase composed of Acetonitrile: Water (90:10; %v/v) adjusted to pH 3.3 with 10% orthophosphoric acid) with flow rate 1 ml/min. Both drugs were eluted, isocratically using detection wavelength 230 nm. Methanol was used as a solvent, the spectrum was recorded between 200-400 nm wavelengths, and all the zero-order spectrum (D0) were converted to first-order derivative spectrum (D1) using delta lambda 2.0 and scaling factor 4. 240 nm (zero crossing point of Fenofibrate) and 306 nm (zero crossing point of Simvastatin) were used for determination of Simvastatin and Fenofibrate, respectively. Regression analysis of UV-Spectrophotometric method showed good linearity r2 = 0.9991 at 240 nm of Simvastatin 1-5 µg/ml and r2 = 0.9998 at 306 nm of Fenofibrate 10-50 µg/ml. For proposed methods, the linearity for both methods were obtained in the concentration range of 1-5 μg/ml for Simvastatin and 10-50 μg/ml for Fenofibrate. Statistical analysis by student’s t-test showed no significance difference between the results obtained by these two methods.  Results: The suitability of method for the quantitative determination of Simvastatin and Fenofibrate was proved by validation. Conclusion: The proposed methods and its results had been successfully applied and validated statistically to the simultaneous estimation of Simvastatin and Fenofibrate in their combination for quality analysi

Characterization of Morinda tomentosa genotypes under rainfed conditions of western India

A field trial was conducted to characterize Morinda tomentosa Heyne ex Roth genotypes which were established during 2009 under field condition during the years 2011-13 at Experimental Farm of Central Horticultural Experiment Station (CIAH-ICAR) Vejalpur, Panchmahals (Godhra), Gujarat. The vegetative morphomatrix in terms of plant height, stem girth, plant spread, number of primary branches and number of secondary branches varied between 3.10-4.70 cm, 19.10-36.08 cm, 2.27-3.87 m, 6.23-13.45 and 12.12-26.00, respectively, whereas leaves morphology, viz. leaf length, breadth, petiole length and vein pairs ranged between 15.00-25.00 cm, 8.00-16.50 cm,1.25-2.29 cm and 7.43-11.33 per leaf, respectively. Yield /plant during 3rd year varied between 3.50-9.41 kg being highest in CHESN 1 and the lowest in CHESN 21 in all the evaluated genotypes. The quantitative fruit characters in terms of fruit weight, length, breadth, number of pyrines, number of seed/fruit and fresh weight of seed ranged between 19.20-47.10 g, 2.49-4.80 cm, 2.34-3.98 cm, 10.95-23.95, 25.17-50.65 and 0.06-0.11 g, respectively. Variations in values of TSS, pH, vitamin C and acidity, protein, phenols, tannins, Ca, K, Na, and Zn ranged between 6-120 brix, 3.50-7.00, 21.15-40.15 mg/ 100g, 1.16-1.51 per cent, 0.09-0.29 g, 11.12-20.05 mg, 0.25-0.46 g, 90.10-102.00 mg, 36.12-49.92 mg, 80.16-93.90 mg and 0.10-0.29 mg among the genotypes characterized for their biochemical composition. Based on the overall observations, CHESN 1, CHESN 16 and CHESN 31 were found to better with respect to most of the qualitative and quantitative characters than rest of the genotypes evaluated under rainfed conditions of western India

FORMULATION AND EVALUATION OF BILAYER TABLETS OF BACLOFEN USING SYNTHETIC POLYMERS

Aim of study was to develop bilayer drug delivery for treatment of spasticity by delivering loading and maintenance dose for fast achievement of peak plasma concentration and maintaining the same respectively. The prepared drug loaded bilayer tablets were evaluated for pre and post compression parameters. Stability study of the promising formulation was also performed. The tablets were prepared by direct compression and wet granulation method. The loading dose was delivered in the form of immediate release layer prepared by different super-disintegrations and maintenance dose was delivered through sustained release layer prepared by using polymers like HPMC K4, HPMC K15M, HPMC K100, PEO WSR 303 and Precirol. Both the immediate release layer and sustained release layers were separately optimized and then combined to optimize the bilayertablets. No interactions were found between drug and excipients. Formulation containing Kyron T314 shows immediate drug release. Formulation Containing PEO WSR 303 shows sustained release action and bilayer formulations F6 shows releases up to 24 hours. Bilayer tablets with release characteristics offer critical advantages such as, site specificity with improved absorption and efficacy.Key words: Baclofen, Bilayer tablet, sustain release table

Stock assessment of Bombayduck Harpadon nehereus (Hamilton, 1822) from Gujarat coast using non-equilibrium production model

Bombayduck Harpadon nehereus, harvested mainly by dol nets (stationary bag nets), has been a prolific fishery in the northern region of Arabian Sea and Bay of Bengal. Biomass and maximum sustainable yield (MSY) estimates for the Bombayduck stock in the Saurashtra region were obtained from a non-equilibrium surplus production model approach utilising catch per unit effort (CPUE) time series derived from fish landing data. Fox model was found to be the most appropriate defining model and the results demonstrated that the stock is currently being overexploited

Occurrence of the snapper Paracaesio sordida Abe & Shinohara, 1962 from north-west coast of India

Paracaesio sordida Abe & Shinohara 1962, the dirty ordure snapper (Family: Lutjanidae), has a wide distribution in the tropical waters with its occurrence reported from Western Central Pacific and the Indian Ocean. From Indian waters, this species has been previously reported along the east coast, Lakshadweep and from Mangalore in the west coast