Zastosowanie peptonu Peptobak-Bacutil tu hodowli merystematycznej tkanki Cymbidium Sw. [Application of the peptone Peptobak-Bacutil medium in the culture of meristem-tissue of Cymbidium Sw.]

In was found that peptone of Peptobak-Bacutil brand (produced in Poland fulfills perfectly the requirements in cultures in vitro of the meristem tissues of Cymbidium in the modified Tsuchiya's medium in a concentration of 2 g per 1. A decrease in the concentration to 1 g per 1. affects the growth intensity of the tissue, but an increase of concentration up to 3 g per 1. retards the transformation of protocorms into the plant-stage. Growth of the meristem tissue of Cymbidium in the medium consolidated with agar is weaker and the number of protocorms produced 2,3 times lower than in liquid media

Modifications in regeneration of Saintpaulia ionantha Wendl. leaves, due to morphactin added to in vitro cultures

The paper reports the results of experiments on the course of leaf regeneratian in Saintpaulia ionantha Wendl. in vitro culture, depending on morphactin IT 3456 doses and its relation with kinetin and auxins (IAA and NAA). Morphactin retards regeneration, abolishes its polarity, inhibits root formation and causes developmental anomalies of buds and leaves. Kinetin and IAA neutralize the retardation of regeneration caused by morphactin, auxin and finetin do not prevent the anomalies

Effect of morphactine on differentiation and development of Cymbidium Sw. protocorms cultured in vitro

The morphogenetic effect of morphactine IT 3456 on meristematic tissue of Cymbidium Sw. was investigated within the dose range 0.01 to 10 ppm in in vitro culture on liquid and agar-solidified medium. In the doses applied, morphactine increased the number of protocorms differentiating from isolated meristematic tissues, and, in dependence on the dose, affected their shape and proliferation. Morphactine retards, and in large doses completely inhibits, the development of rhizoids, shoots and roots. Various developmental anomalies were observed under the influence of morphactine: leaf syncotylia, and their deformation, thickening, shortening, flattening and branching of shoots as well as formation of 2-3 shoots by one protocorm and development of secondary protocorms on the leaves

Heterogeneity of the acid phosphatase and ribonuclease from protocorms of the orchids Cymbidium Sw. and changes occurring after treatment with streptomycin

The molecular forms of the acid phosphatase and RNase in protocorms of Cymbidium Sw. were studied by disc electrophoresis. The effect of streptomycin added to the culture medium on both enzymes was investigated. Significant changes in enzyme activity and electrophoretic patterns occured after addition of streptomycin at the beginning of culture growth. This indicates that the enzymes are affected by streptomycin in early stages of development of the protocorms

Heterogeneity of the acid phosphatase and ribonuclease from protocorms of the orchids Cymbidium Sw. and changes occurring after treatment with streptomycin

The molecular forms of the acid phosphatase and RNase in protocorms of Cymbidium Sw. were studied by disc electrophoresis. The effect of streptomycin added to the culture medium on both enzymes was investigated. Significant changes in enzyme activity and electrophoretic patterns occured after addition of streptomycin at the beginning of culture growth. This indicates that the enzymes are affected by streptomycin in early stages of development of the protocorms