56 research outputs found
Problemi metodologici nel monitoraggio dell’inquinamento batterico in ambiente marino costiero
Comparison of putative virulence factors in Aeromonas hydrophila strains isolated from the marine environment and human diarrheal cases in southern Italy
An outbreak of Aeromonas hydrophila infection in turtles (Pseudemis scripta)
An outbreak of Aeromonas hydrophila infection with a high rate of mortality (95%) in turtles (Pseudemis
scripta) in Italy is reported. Pure cultures of the pathogen were isolated from liver, lung, kidney, and heart
specimens of the turtles. The A. hydrophila isolate was resistant to amoxicillin, ampicillin, cephalothin, and
trimethoprim-sulfamethoxazole but was sensitive to a number of other antibiotics tested. The study indicates
that pet turtles can act as reservoirs of this pathogen and may play an important role in the etiology of
Aeromonas-associated human infection
Fatal septicaemia caused by Aeromonas hydrophila in a patient with cirrhosis
In this case report from Italy we describe a fatal infection caused by A. hydrophila in a 39 yr old cirrhotic patient. This pathogen was isolated as a pure single culture from the patient's blood sample. The patient died on the second day of hospitalization from overwhelming sepsis. The A. hydrophila isolate was tested for different potential virulence properties, such as invasiveness, adherence, exotoxins production, presence of fimbriae and for the patterns of resistance to a variety of antimicrobial agents. Although, the Aeromonas species are infrequently reported as a cause of human infections, the present case study confirms the capability of these pathogens to induce serious human infections
Molecular typing and antimicrobial susceptibility of Vibrio parahaemolyticus strains
The aim of the present study was to use three PCR-based techniques for the analysis of genetic variability among Vibrio parahaemolyticus strains isolated from the Philippines. Seventeen strains of V. parahaemolyticus isolated from shrimps and from the environments where these shrimps are being cultivated were analyzed by RAPD, ERIC and REP-PCR. Antimicrobial susceptibility of these strains to selected compounds was investigated using broth microdilution method. Results of this work and analysis of similarity among strains using Dice coefficient and unweighted average pair group method have demonstrated genetic variability within the V. parahaemolyticus strains. The RAPD, ERIC and REP-PCR were found to be suitable typing methods for V. parahaemolyticus. They have good discriminative ability and can be used as rapid means of comparing these strains for epidemiological investigation. However, the REP-PCR analysis yielded a relatively small number of products suggesting that the REP sequences may not be widely distributed in the V. parahaemolyticus genome. Results of antimicrobial susceptibility revealed that resistance among the strains was rare. In conclusion, RAPD, ERIC and REP-PCR techniques are useful methods for molecular typing of V. parahaemolyticus strains. To our knowledge this is the first study of this kind carried out on V. parahaemolyticus strains isolated from the Philippines
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