LEA (Late Embryogenesis Abundant) proteins and their encoding genes in Arabidopsis thaliana

<p>Abstract</p> <p>Background</p> <p>LEA (late embryogenesis abundant) proteins have first been described about 25 years ago as accumulating late in plant seed development. They were later found in vegetative plant tissues following environmental stress and also in desiccation tolerant bacteria and invertebrates. Although they are widely assumed to play crucial roles in cellular dehydration tolerance, their physiological and biochemical functions are largely unknown.</p> <p>Results</p> <p>We present a genome-wide analysis of LEA proteins and their encoding genes in <it>Arabidopsis thaliana</it>. We identified 51 LEA protein encoding genes in the Arabidopsis genome that could be classified into nine distinct groups. Expression studies were performed on all genes at different developmental stages, in different plant organs and under different stress and hormone treatments using quantitative RT-PCR. We found evidence of expression for all 51 genes. There was only little overlap between genes expressed in vegetative tissues and in seeds and expression levels were generally higher in seeds. Most genes encoding LEA proteins had abscisic acid response (ABRE) and/or low temperature response (LTRE) elements in their promoters and many genes containing the respective promoter elements were induced by abscisic acid, cold or drought. We also found that 33% of all Arabidopsis LEA protein encoding genes are arranged in tandem repeats and that 43% are part of homeologous pairs. The majority of LEA proteins were predicted to be highly hydrophilic and natively unstructured, but some were predicted to be folded.</p> <p>Conclusion</p> <p>The analyses indicate a wide range of sequence diversity, intracellular localizations, and expression patterns. The high fraction of retained duplicate genes and the inferred functional diversification indicate that they confer an evolutionary advantage for an organism under varying stressful environmental conditions. This comprehensive analysis will be an important starting point for future efforts to elucidate the functional role of these enigmatic proteins.</p

On the negative spectrum of two-dimensional Schr\"odinger operators with radial potentials

For a two-dimensional Schr\"odinger operator $H_{\alpha V}=-\Delta-\alpha V$ with the radial potential $V(x)=F(|x|), F(r)\ge 0$, we study the behavior of the number $N_-(H_{\alpha V})$ of its negative eigenvalues, as the coupling parameter $\alpha$ tends to infinity. We obtain the necessary and sufficient conditions for the semi-classical growth $N_-(H_{\alpha V})=O(\alpha)$ and for the validity of the Weyl asymptotic law.Comment: 13 page

Localization criteria for Anderson models on locally finite graphs

We prove spectral and dynamical localization for Anderson models on locally finite graphs using the fractional moment method. Our theorems extend earlier results on localization for the Anderson model on \ZZ^d. We establish geometric assumptions for the underlying graph such that localization can be proven in the case of sufficiently large disorder

The role of raffinose in the cold acclimation response of Arabidopsis thaliana

AbstractIn many plants raffinose family oligosaccharides are accumulated during cold acclimation. The contribution of raffinose accumulation to freezing tolerance is not clear. Here, we investigated whether synthesis of raffinose is an essential component for acquiring frost tolerance. We created transgenic lines of Arabidopsis thaliana accessions Columbia-0 and Cape Verde Islands constitutively overexpressing a galactinol synthase (GS) gene from cucumber. GS overexpressing lines contained up to 20 times as much raffinose as the respective wild-type under non-acclimated conditions and up to 2.3 times more after 14 days of cold acclimation at 4 °C. Furthermore, we used a mutant carrying a knockout of the endogenous raffinose synthase (RS) gene. Raffinose was completely absent in this mutant. However, neither the freezing tolerance of non-acclimated leaves, nor their ability to cold acclimate were influenced in the RS mutant or in the GS overexpressing lines. We conclude that raffinose is not essential for basic freezing tolerance or for cold acclimation of A. thaliana

Upper and lower limits on the number of bound states in a central potential

In a recent paper new upper and lower limits were given, in the context of the Schr\"{o}dinger or Klein-Gordon equations, for the number $N_{0}$ of S-wave bound states possessed by a monotonically nondecreasing central potential vanishing at infinity. In this paper these results are extended to the number $N_{\ell}$ of bound states for the $\ell$-th partial wave, and results are also obtained for potentials that are not monotonic and even somewhere positive. New results are also obtained for the case treated previously, including the remarkably neat \textit{lower} limit $N_{\ell}\geq \{\{[\sigma /(2\ell+1)+1]/2\}\}$ with $V(r)| ^{1/2}]$ (valid in the Schr\"{o}dinger case, for a class of potentials that includes the monotonically nondecreasing ones), entailing the following \textit{lower} limit for the total number $N$ of bound states possessed by a monotonically nondecreasing central potential vanishing at infinity: N\geq \{\{(\sigma+1)/2\}\} {(\sigma+3)/2\} \}/2 (here the double braces denote of course the integer part).Comment: 44 pages, 5 figure

Bound States in one and two Spatial Dimensions

In this paper we study the number of bound states for potentials in one and two spatial dimensions. We first show that in addition to the well-known fact that an arbitrarily weak attractive potential has a bound state, it is easy to construct examples where weak potentials have an infinite number of bound states. These examples have potentials which decrease at infinity faster than expected. Using somewhat stronger conditions, we derive explicit bounds on the number of bound states in one dimension, using known results for the three-dimensional zero angular momentum. A change of variables which allows us to go from the one-dimensional case to that of two dimensions results in a bound for the zero angular momentum case. Finally, we obtain a bound on the total number of bound states in two dimensions, first for the radial case and then, under stronger conditions, for the non-central case.Comment: Latex, 27pp no figure

Inferring statistics of planet populations by means of automated microlensing searches

(abridged) The study of other worlds is key to understanding our own, and not only provides clues to the origin of our civilization, but also looks into its future. Rather than in identifying nearby systems and learning about their individual properties, the main value of the technique of gravitational microlensing is in obtaining the statistics of planetary populations within the Milky Way and beyond. Only the complementarity of different techniques currently employed promises to yield a complete picture of planet formation that has sufficient predictive power to let us understand how habitable worlds like ours evolve, and how abundant such systems are in the Universe. A cooperative three-step strategy of survey, follow-up, and anomaly monitoring of microlensing targets, realized by means of an automated expert system and a network of ground-based telescopes is ready right now to be used to obtain a first census of cool planets with masses reaching even below that of Earth orbiting K and M dwarfs in two distinct stellar populations, namely the Galactic bulge and disk. The hunt for extra-solar planets acts as a principal science driver for time-domain astronomy with robotic-telescope networks adopting fully-automated strategies. Several initiatives, both into facilities as well as into advanced software and strategies, are supposed to see the capabilities of gravitational microlensing programmes step-wise increasing over the next 10 years. New opportunities will show up with high-precision astrometry becoming available and studying the abundance of planets around stars in neighbouring galaxies becoming possible. Finally, we should not miss out on sharing the vision with the general public, and make its realization to profit not only the scientists but all the wider society.Comment: 10 pages in PDF format. White paper submitted to ESA's Exo-Planet Roadmap Advisory Team (EPR-AT); typos corrected. The embedded figures are available from the author on request. See also "Towards A Census of Earth-mass Exo-planets with Gravitational Microlensing" by J.P. Beaulieu, E. Kerins, S. Mao et al. (arXiv:0808.0005

Sufficient conditions for two-dimensional localization by arbitrarily weak defects in periodic potentials with band gaps

We prove, via an elementary variational method, 1d and 2d localization within the band gaps of a periodic Schrodinger operator for any mostly negative or mostly positive defect potential, V, whose depth is not too great compared to the size of the gap. In a similar way, we also prove sufficient conditions for 1d and 2d localization below the ground state of such an operator. Furthermore, we extend our results to 1d and 2d localization in d dimensions; for example, a linear or planar defect in a 3d crystal. For the case of D-fold degenerate band edges, we also give sufficient conditions for localization of up to D states.Comment: 9 pages, 3 figure

Variable stars in the bulge globular cluster NGC 6401

We present a study of variable stars in globular cluster NGC 6401. The cluster is only 5.3º away from the Galactic centre and suffers from strong differential reddening. The photometric precision afforded us by difference image analysis resulted in improved sensitivity to variability in formerly inaccessible interior regions of the cluster. We find 23 RRab and 11 RRc stars within one cluster radius (2.4'), for which we provide coordinates, finder-charts and time-series photometry. Through Fourier decomposition of the RR Lyrae star light curves we derive a mean metallicity of [Fe/H]UVES = -1.13 ± 0.06 ([Fe/H]ZW = -1.25 ± 0.06), and a distance of d ≈ 6.35 ± 0.81 kpc. Using the RR Lyrae population, we also determine that NGC 6401 is an Oosterhoff type I cluster.PostprintPeer reviewe