Long, lifetime, triggered, spark-gap switch for repetitive pulsed power applications

In this article a critical component for pulsed power applications is described: the heavy-duty switch. The design of a coaxial, high repetition rate, large average power, and long lifetime spark-gap switch is discussed. The switch is used with a fail-free LCR trigger circuit. Critical issues for switch design are presented together with experimental results. It is observed that the switch has a good stability, and its lifetime is estimated to be in the order of 1010 shots (~106 C) at 10 J/pulse, 60 kV and 100 ns pulses. Measurements were performed with 20 and 34 kV average switching voltage (100 ns pulses, energy per pulse 0.4 and 0.75 J, respectively). For up to 450 pulses/s (pps), pre-firing can be prevented by increasing the gap pressure (up to 2.5 and 7 bars, respectively), no gas flush is required. Above 450 pps, up to 820 pps, a forced gas flow of maximal 35 Nm3/h, is required for stable operation. Measurements on the time delay and jitter of the switch demonstrate that these values are influenced by pressure, flow, and pulse repetition rate. For 34 kV average switching voltage the time delay and time jitter vary between 35 and 250 and 10 and 80 µs, respectively. For 20 kV average switching voltage these values are: 30–160 and 4–50 µs. During a test run of 2.5 h (at 100 Hz, 0.75 J/pulse) the feasibility of the switch was proved, and the switching voltage jitter was less than 0.7%. ©2005 American Institute of Physic

Factors influencing noncompliance with bicycle passing distance laws

Many jurisdictions around the world have implemented laws to require a minimum distance when motor vehicles pass cyclists, but research into the factors influencing passing distances has produced inconsistent results, indicating the need for future research. This study examined the factors influencing motorists’compliance with a legislated bicycle passing distance rule in Queensland, Australia. Unlike the earlier studies, which used volunteer riders to record passing events, this study used a naturalistic study design to record passing events where none of the motorists or the cyclists were aware of being studied. As a result, this study captured the ‘true’driving and riding behaviours during passing events. The likelihood of non-compliance was greater on higher (70–80 km/h speed limits) and lower (40 km/h) speed roads than 60 km/h roads, at curved road sections, and on roads with narrower traffic lanes. Rider characteristics (age, gender, helmet status, type of clothing, type of bicycle, and individual or group riding) had no statistically significant association with compliance status. The findings indicate that efforts to improve cyclist safety during overtaking events should focus on non-rider related factors, such as roadway infrastructure characteristics

Differential effects of familial Alzheimer’s disease-causing mutations on amyloid precursor protein (APP) trafficking, proteolytic conversion, and synaptogenic activity

The amyloid precursor protein (APP) is a key player in Alzheimer`s disease (AD) and the precursor of the Aβ peptide, which is generated by consecutive cleavages of β- and γ-secretases. Familial Alzheimer’s disease (FAD) describes a hereditary subgroup of AD that represents a low percentage of AD cases with an early onset of the disease. Different APP FAD mutations are thought to have qualitatively different effects on its proteolytic conversion. However, few studies have explored the pathogenic and putative physiological differences in more detail. Here, we compared different FAD mutations, located at the β- (Swedish), α- (Flemish, Arctic, Iowa) or γ-secretase (Iberian) cleavage sites. We examined heterologous expression of APP WT and FAD mutants in non-neuronal cells and their impact on presynaptic differentiation in contacting axons of co-cultured neurons. To decipher the underlying molecular mechanism, we tested the subcellular localization, the endocytosis rate and the proteolytic processing in detail by immunoprecipitation–mass spectrometry. Interestingly, we found that only the Iberian mutation showed altered synaptogenic function. Furthermore, the APP Iowa mutant shows significantly decreased α-secretase processing which is in line with our results that APP carrying the Iowa mutation was significantly increased in early endosomes. However, most interestingly, immunoprecipitation–mass spectrometry analysis revealed that the amino acid substitutions of APP FAD mutants have a decisive impact on their processing reflected in altered Aβ profiles. Importantly, N-terminally truncated Aβ peptides starting at position 5 were detected preferentially for APP Flemish, Arctic, and Iowa mutants containing amino acid substitutions around the α-secretase cleavage site. The strongest change in the ratio of Aβ40/Aβ42 was observed for the Iberian mutation while APP Swedish showed a substantial increase in Aβ1–17 peptides. Together, our data indicate that familial AD mutations located at the α-, β-, and γ-secretase cleavage sites show considerable differences in the underlying pathogenic mechanisms