Micro Electrical Impedance Spectroscopy of Bovine Chromaffin Cells

Single cell characterization using electrical impedance spectroscopy (EIS) has been conducted on bovine chromaffin cells. Measurements were taken using a micro scale electrophysiological analysis system fabricated using micromachining (e.g. MEMS) technologies. A population of chromaffin cells were injected into a microreservoir and subsequently manipulated into a microchannel with a cross-sectional area of 7.0×7.0 μm2. The microchannel was designed with integrated, opposing electroplated palladium electrodes with a cross-sectional area of 7.0×7.0 μm 2. The electrodes are designed to be in contact with the cells during analysis procedures. Impedance measurements were taken over a frequency range of 40 Hz to 3.0 MHz. Magnitude and phase were studied for a statistically relevant population of chromaffin cells. The impedance spectrums revealed three distinct populations of chromaffin cells. The data obtained for the chromaffin cells was compared to data obtained for red blood cells, air, and phosphate buffered solution

A Micro System Using Dielectrophoresis and Electrical Impedance Spectroscopy for Cell Manipulation and Analysis

Single cell manipulation and analysis using dielectrophoresis (DEP) and micro electrical impedance spectroscopy (µ-EIS) has been demonstrated on bovine chromaffin cells and red blood cells. Cells were manipulated and analyzed on a micro scale electrophysiological analysis system fabricated using micromachining (e.g. MEMS) technologies. Cells were injected into a microreservoir and collected for µ-EIS analysis using vacuum and DEP. Impedance measurements were taken over a frequency range of 40 Hz to 3.0 MHz. The data obtained for the cells was compared to data obtained for air, and phosphate buffered solution. Data for chromaffin cells was analyzed further, assessing cell size and calcium ion channel activity in relation to impedance spectroscopy

A Micro System Using Dielectrophoresis and Electrical Impedance Spectroscopy for Cell Manipulation and Analysis

Single cell manipulation and analysis using dielectrophoresis (DEP) and micro electrical impedance spectroscopy (µ-EIS) has been demonstrated on bovine chromaffin cells and red blood cells. Cells were manipulated and analyzed on a micro scale electrophysiological analysis system fabricated using micromachining (e.g. MEMS) technologies. Cells were injected into a microreservoir and collected for µ-EIS analysis using vacuum and DEP. Impedance measurements were taken over a frequency range of 40 Hz to 3.0 MHz. The data obtained for the cells was compared to data obtained for air, and phosphate buffered solution. Data for chromaffin cells was analyzed further, assessing cell size and calcium ion channel activity in relation to impedance spectroscopy

Micro Electrical Impedance Spectroscopy of Bovine Chromaffin Cells

Single cell characterization using electrical impedance spectroscopy (EIS) has been conducted on bovine chromaffin cells. Measurements were taken using a micro scale electrophysiological analysis system fabricated using micromachining (e.g. MEMS) technologies. A population of chromaffin cells were injected into a microreservoir and subsequently manipulated into a microchannel with a cross-sectional area of 7.0×7.0 μm2. The microchannel was designed with integrated, opposing electroplated palladium electrodes with a cross-sectional area of 7.0×7.0 μm 2. The electrodes are designed to be in contact with the cells during analysis procedures. Impedance measurements were taken over a frequency range of 40 Hz to 3.0 MHz. Magnitude and phase were studied for a statistically relevant population of chromaffin cells. The impedance spectrums revealed three distinct populations of chromaffin cells. The data obtained for the chromaffin cells was compared to data obtained for red blood cells, air, and phosphate buffered solution