Relations of immunohistochemical expression of ICAM-1, VCAM-1, Cyclin D1 and Cathepsin D with axillary lymph node metastases and prognostic factors in invasive ductal breast carcinomas

Bu çalışma 153 adet invaziv duktal meme karsinomu olgusunda yaş, sağ veya sol memede yerleşim,tümör çapı, Paget hastalığı, metastatik aksiller lenf nodu sayısı, metastatik lenf nodlarında kapsül invazyonu, histolojik grade, odak sayısı, DKİS, kadran yerleşimi paramatrelerin tespiti sonrasında İHK yöntem ile belirlenen İCAM-1, VCAM-1, SD1, KD ve daha önceden İHK olarak boyanan ER, PR, cerb-B2 ekspresyonlarının ilişkilerini ve varsa birbirlerine olan üstünlüklerini değerlendirmeyi amaçlamıştır.Aksiller lenf nodu metastazı ve kapsül invazyonu olanlar (grup 1), aksiller lenf nodu metastazı olup kapsül invazyonu olmayanlar (grup 2) ve aksiller lenf nodu metastazı olmayanlar (grup 3) olmak üzere 3 grup oluşturulmuştur.İCAM-1 boyanması negatif vakalarda cerb-B2 negatifliği dikkati çekmiş olup, cerb-B2 ile VCAM-1, SD1 ve KD arasında ilişki tespit edilmedi.ER pozitifliğinin kuvvetli olduğu vakalarda VCAM-1 pozitifliğinin belirgin olduğu dikkati çekmiş olup, ER ile İCAM-1, SD1 ve KD arasında ilişki tespit edilmedi.PR pozitifliğinin kuvvetli olduğu vakalarda VCAM-1 pozitifliğinin belirgin olduğu dikkati çekmiş olup, PR ile İCAM-1, SD1 ve KD arasında ilişki tespit edilmedi.Metastatik aksiller lenf nodlarında kapsül invazyonu olan vakalarda KD pozitifliğinin belirgin olduğu izlenmiş olup, metastatik lenf nodlarında kapsül invazyonu ile İCAM-1, VCAM-1 ve SD1 arasında ilişki tespit edilmedi.Grup 1'de metastatik aksiller lenf nodu sayısının daha fazla ve KD pozitifliğinin belirgin olduğu dikkati çekmiştir.Yaş, sağ veya sol memede yerleşim, tümör çapı, Paget hastalığı, metastatik aksiller lenf nodu sayısı, histolojik grade, odak sayısı, DKİS, kadran yerleşimi paramatreleri ile İCAM-1, VCAM-1, SD1 ve KD negatif ve pozitif boyanan gruplar arasında istatiksel olarak ilişki tespit edilmedi.Anahtar kelimeler: İnvaziv duktal meme karsinomu, prognostik faktörler, İCAM-1, VCAM-1, SD1, KDIt was aimed to compare ICAM-1(intercellular adhesion molecule-1), VCAM-1(vascular cell adhesion molecule-1), SD1(cyclin D1), KD(cathepsin D) with expression degrees and ER (estrogen receptor), PR(progesterone receptor), cerb-B2 (HER-2/neu) expression which had been evaluated before, and to determine relationship between these İHK (immunhistochemistry) findings after establishing age, right or left side localization, tumor size, Paget disease, number of metastatic axillary lymph nodes, invasion of capsule in metastatic axillary lymph nodes, histological grade, number of focus, presence of DCIS (ductal carcinoma in situ), quadrant localization in 153 cases with invasive ductal breast carcinoma in this study.Three group was formed; the cases with invasion of capsule in metastatic axillary lymph nodes were called as group 1, the cases with was axillary nodal metastasis, without capsule invasion, were cases called as group 2, and with no axillary nodal metastasis, were called as group 3.We established that the cases which were negative for ICAM-1, were negativite for cerb-B2. However, there was no association between cerb-B2 and VCAM-1, SD1, KD.In cases which were strongly positive for PR, were positivite for VCAM-1. There was no association between PR and ICAM-1, SD1, KD.In the cases with capsule invasion in axillary lymph nodes; strongly positive with KD was determined. There was no association between capsule invasion in metastatic axillary lymph nodes and ICAM-1, SD1, KD expression.In group 1, a higher frequency of axillary nodal metastasis and strongly positivite with KD was significant.There was no statistically significant difference between negative and positive groups with ICAM-1, VCAM-1, SD1 and KD. Also, there was no statistically significant difference between parameters such as age, right or left side localization, tumor size, Paget disease, number of metastatic lymph nodes, histological grade, number of focus, presence of DCIS, quadrant localization.Key Words: Invasive ductal breast carcinoma, prognostic factors, ICAM-1, VCAM-1, SD1, KD

Association of ICAM-1, VCAM-1, CYCLIN D1 and Cathepsin D with Clinicopathological Parameters in Breast Carcinoma; an Immunohistochemical Study

WOS:000395502400002PubMed ID: 28331761Objective: Breast carcinoma is the most common malignant tumor detected in women. fte hypothesis that increased levels of adhesion molecules and Cathepsin D affect cancerous cells moving away the primary tumor and contributes to migration of the cancerous cell and may cause remote organ metastases is defended. fte aim of the present study was to search the association of intracellular adhesion molecule-1 (ICAM-1), vascular adhesion molecule-1 (VCAM-1), Cyclin D1, cathepsin D immunohistochemically with clinicopathological parameters in the patients diagnosed with invasive ductal breast carcinoma.Materials and Methods: fte pathological slides of 153 patients diagnosed with invasive ductal carcinoma were evaluated retrospectively. ftree groups were created. Group 1 consisted of patients with positive lymph node metastasis and extranodal tumor invasion; Group 2 consisted of patients with positive axillary lymph node metastasis and negative extranodal tumor invasion and Group 3 consisted of the patients with negative axillary lymph node metastasis. In all groups, 20 paraffin blocks belonging to the primary tumor in the breast were stained by ICAM-1, VCAM-1, Cyclin D1 and Cathepsin D. Findings were examined by comparing with clinicopathological parameters.Results: fte highest number of metastatic axillary lymph nodes and the highest rate of cathepsin D staining were statistically found in the cases with positive axillary lymph node metastasis and extranodal tumor invasion. CerbB2 was negative in the cases with negative ICAM-1 whereas estro- gen receptor and progesterone receptor were positive in the cases with positive VCAM-1.Conclusion: fte present study reveals significant results for the patients diagnosed with invasive ductal carcinoma through breast biopsy espe- cially before mastectomy in terms of increased number of metastatic axillary lymph nodes and extranodal tumor invasion by immunohistochemical Cathepsin D stain without any additional invasive intervention. Results of the present study may contribute to monitoring and treatment of the patients in the future

Adrenal myelolipoma - evaluating of four cases

Adrenal myelolipom, genellikle tek taraflı, düzgün sınırlı, değişken boyutlarda olan, adrenal bezin nadir, benign ve hormonal olarak inaktif bir tümörüdür. Neoplazm yağ doku ve kemik iliğine ait myelopoetik hücrelerden olu- şur. Pekçok tümör asemptomatiktir ve çoğunlukla otopsi çalışmalarında tesadüfen tespit edilir. Dört tane adrenal myelolipom vakamızı, makroskopik ve mikroskopik özelliklerini vurgulayarak sunduk.Adrenal myelolipoma is a rare, benign and hormonally inactive tumor of the adrenal gland, usually unilateral, well limited, variable in size. The neoplasm consists of adipose tissue and myelopoietic cells of the bone marrow Most tumors are asymptomatic and usually are discovered incidentally at autopsy studies. We have reported four cases of adrenal myelolipoma, emphasizing their macroscopic and microscopic features

Adrenal myelolipoma - evaluating of four cases

Adrenal myelolipom, genellikle tek taraflı, düzgün sınırlı, değişken boyutlarda olan, adrenal bezin nadir, benign ve hormonal olarak inaktif bir tümörüdür. Neoplazm yağ doku ve kemik iliğine ait myelopoetik hücrelerden olu- şur. Pekçok tümör asemptomatiktir ve çoğunlukla otopsi çalışmalarında tesadüfen tespit edilir. Dört tane adrenal myelolipom vakamızı, makroskopik ve mikroskopik özelliklerini vurgulayarak sunduk.Adrenal myelolipoma is a rare, benign and hormonally inactive tumor of the adrenal gland, usually unilateral, well limited, variable in size. The neoplasm consists of adipose tissue and myelopoietic cells of the bone marrow Most tumors are asymptomatic and usually are discovered incidentally at autopsy studies. We have reported four cases of adrenal myelolipoma, emphasizing their macroscopic and microscopic features