Synergistic effects of Cinnamomum cassia L. essential oil in combination with polymyxin B against carbapenemase-producing Klebsiella pneumoniae and Serratia marcescens.

Multidrug resistance prompts the search for new sources of antibiotics with new targets at bacteria cell. To investigate the antibacterial activity of Cinnamomum cassia L. essential oil (CCeo) alone and in combination with antibiotics against carbapenemase-producing Klebsiella pneumoniae and Serratia marcescens. The antimicrobial susceptibility of the strains was determined by Vitek® 2 and confirmed by MALDI-TOF/TOF. The antibacterial activity of CCeo and its synergism with antibiotics was determined using agar disk diffusion, broth microdilution, time-kill, and checkboard methods. The integrity of the bacterial cell membrane in S. marcescens was monitored by protein leakage assay. CCeo exhibited inhibitory effects with MIC = 281.25 μg.mL-1. The association between CCeo and polymyxin B showed a decrease in terms of viable cell counts on survival curves over time after a 4 hour-treatment with a FIC index value of 0.006. Protein leakage was observed with increasing concentrations for CCeo and CCeo + polymyxin B treatments. CCeo showed antibacterial activity against the studied strains. When associated with polymyxin B, a synergistic effect was able to inhibit bacterial growth rapidly and consistently, making it a potential candidate for the development of an alternative treatment and drug delivery system for carbapenemase-producing strains

Advancing co-production for transformative change by synthesizing guidance from case studies on the sustainable management and governance of natural resources

Co-production has become paramount for scientists, practitioners and social groups of Indigenous peoples and local communities of rural and urban areas to deliver transformative changes that enhance sustainability. Co-production should result in knowledge that is credible, legitimate and usable to enable sustainable outcomes effectively. However, this is not always the case due to challenges related to differences between scientific and Indigenous and local knowledge, as well as inherent power imbalances. The literature emphasises that these challenges are often triggered by rigid scientific theories and postures, dominant practices, and time-money limitations that co-production projects involve. This happens despite the adoption of guidelines recommended in the literature. We investigate the role of these challenges and guidelines in the generation of credible, legitimate, usable, and effective knowledge. We analyse this role in 13 co-production cases focused on sustainable transformative changes linked with the management and governance of natural resources across the globe. Despite challenges varying between groups and contexts, credibility, usability, and effectiveness are promoted simultaneously, especially when co-production empowers social actors via legitimate processes. Scientists and practitioners do so, through creative and flexible reshaping of existing knowledge and worldviews with a focus on common goals that link sustainability and livelihoods. They conceptualise a mutual understanding of knowledge and that is deemed trustworthy feasible to use in their socioecological context. Our findings complement existing scholarship on co-production, exploring the credibility of situated knowledge and its practical effectiveness together with its commonly addressed legitimacy and usability. A focus on the practices of different actors, including dynamics that are external to co-production, and changes in the scientific and social status quo, are needed to advance co-production effectiveness